FCS, fetal calf serum
Effects of the epigenetic drug MS-275 on the release and function of exosome-related immune molecules in hepatocellular carcinoma cells
... FBS: Fetal bovine serum; FCS: Fetal calf serum; HDACi: histone deacetylase inhibitor; HepG2: Hepatoma G2; HSP: heat shock protein; IL: Interleukin; MHC: Major histocompatibility ...
7
Safety and tolerance of lymph node biopsies from chronic HIV 1 volunteers in rural Tanzania
... sulfoxide; FCS: fetal calf serum; GC: germinal center; HIV‑1: human immunodeficiency virus type 1; KIULARCO: Kilombero and Ulanga Antiretroviral Cohort; LN: lymph node; LNMC: lymph node ...
5
Intracellular free radical production by peripheral blood T lymphocytes from patients with systemic sclerosis: role of NADPH oxidase and ERK1/2
... iodonium; FCS: fetal calf serum; FITC: fluorescein isothiocyanate; ERK: extracellular signal-regulated kinase; IFN α : interferon alpha; IL: interleukin; NAC: N-acetylcysteine; NOX2: NADPH ...
12
High carriage of adherent invasive E. coli in wildlife and healthy individuals
... AIEC: adherent invasive Escherichia coli; BME: Basal medium eagle; CD: Crohn’s disease; ECOR: Escherichia coli Reference Collection; FCS: Fetal calf serum; I-407: Intestine-407 cell li[r] ...
11
Regulation of the fibrinolytic system of cultured human vascular endothelium by interleukin 1
... Abbreviations used in this paper: CM, conditioned medium; ECGF, endothelial cell growth factor; FCS, fetal calf serum; hmIL-l, human monocyte-derived IL-l; HSaVEC, human saphenous vein e[r] ...
6
Ilowski, Maren (2013): Der hepatotrophe Wachstumsfaktor Augmenter of liver regeneration (ALR) wirkt leberspezifisch protektiv gegenüber metabolischen Noxen. Dissertation, LMU München: Medizinische Fakultät
... Abbreviations: rhALR, recombinant human augmenter of liver regeneration; EGF, epidermal growth factor; ERK-1/2, extracellular signal–regulated kinase 1/2; FCS, fetal calf serum; HGF, hep[r] ...
40
Human recombinant interleukin 1 stimulates collagenase and prostaglandin E2 production by human synovial cells
... Abbreviations used in this paper: DME, Dulbecco's modified Eagle's medium; EP, endogenous pyrogen; FCS, fetal calf serum; hrIL- 1, human recombinant interleukin 1; IL-1, interleukin 1; L[r] ...
5
Flow cytometry-based FRET identifies binding intensities in PPARγ1 protein-protein interactions in living cells
... acid; FCS: fetal calf serum; FLIM: fluorescence lifetime imaging microscopy; FRET: Förster´s resonance energy transfer; FSC: forward scatter; GFP: green fluorescent proteins; GPS2: G-protein ...
20
In vitro antigen induced antibody responses to hepatitis B surface antigen in man Kinetic and cellular requirements
... Abbreviations used in this paper: anti-HBs IgG, antibody to hepatitis B surface antigen; ELISA, enzyme-linked immunosorbent assay; FCS, fetal calf serum; HBsAg, hepatitis B surface antig[r] ...
11
Research on advanced intervention using novel bone marrOW stem cell (RAINBOW): a study protocol for a phase I, open-label, uncontrolled, dose-response trial of autologous bone marrow stromal cell transplantation in patients with acute ischemic stroke
... The first clinical trial using autologous BMSC therapy in patients with stroke was reported in 2005. Bang et al. showed the feasibility and safety of the therapy [7]. They adopted cell culture methods with fetal ...
8
Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection
... 20% fetal calf serum (FCS; Biological Industries, Kibbutz Beit Haemek, Israel) in a 1:20 ...20% FCS and cultured immediately in a 25 cm 2 tissue culture flask with the same media in ...
17
The effects of hyperoxia on microvascular endothelial cell proliferation and production of vaso-active substances
... The study was executed in accordance with the Declaration of Helsinki and was ap- proved by the University Human Subjects Committee of the VU University Medical Center. Written informed consent was obtained from all ...
17
Imaging the Antistaphylococcal Activity of CATH-2: Mechanism of Attack and Regulation of Inflammatory Response
... 10% fetal calf serum (FCS), the antimicrobial activity of CATH-2 was assessed under the same conditions, which showed a dose-dependent killing pattern with an MBC of ...
14
Level of muscle regeneration in limb-girdle muscular dystrophy type 2I relates to genotype and clinical severity
... For immunohistochemistry, all sections were fixed in a 1:1 mixture of acetone and methanol or in 4% parafor- maldehyde (for MyoD), and were subsequently blocked using 3% FCS in PBS, then stained. Primary ...
10
ROCK activity and the Gβγ complex mediate chemotactic migration of mouse bone marrow-derived stromal cells
... % fetal bovine serum (HyClone, part of GE Healthcare, Little Chalfont, UK), 10 % equine serum (HyClone), 1 % L-glutamine, and 1 % ...% fetal bovine serum (Gibco) and 1 % antibiotics ...
15
NF κB inhibitor dehydroxymethylepoxyquinomicin suppresses osteoclastogenesis and expression of NFATc1 in mouse arthritis without affecting expression of RANKL, osteoprotegerin or macrophage colony stimulating factor
... We previously demonstrated that 10 μg/ml DHMEQ sup- presses expression of IL-1β and IL-6, as well as CC chemokines CCL2 and CCL5, in culture of TNFα-stimulated RA-FLS [16]. We therefore speculated that expression of M- ...
10
A perfused human blood–brain barrier on-a-chip for high-throughput assessment of barrier function and antibody transport
... Cultures in the OrganoPlate were fixed with 100% meth- anol (− 20 °C, 494437, Sigma) and permeabilized with 0.3% Triton X-100 (T8787, Sigma). Cells were incu- bated with blocking solution (2% FCS, 2% bovine ...
12
Adrenomedullin increases fibroblast like synoviocyte adhesion to extracellular matrix proteins by upregulating integrin activation
... At confluence, FLSs were harvested with trypsin-EDTA, pelleted, and resuspended in HAM-F12 without FCS. FLSs were incubated with adrenomedullin for 1 hour, and 10 5 cells were seeded in triplicate for 2 hours. ...
11
Anti carbamylated protein antibodies precede disease onset in monkeys with collagen induced arthritis
... in serum in up to 46% of patients with RA in several cohorts around the world and are shown to be associated with the development of joint damage ...human serum albumin, fibrinogen and alpha-1 antitrypsin ...
7
Comparison of methods to examine the endogenous peptides of fetal calf serum
... Simple methods that have high yields and good resolving power are required to discover low abundance endogenous peptides. C18 chro- matography worked well alone, or for collecting peptides previously fractionated by ...
23