[FeFe]-hydrogenase
![New Approaches to an Old Problem: Original Techniques in [FeFe]-hydrogenase Research](data:image/gif;base64,R0lGODlhAQABAIAAAP///wAAACH5BAEAAAAALAAAAAABAAEAAAICRAEAOw==)
New Approaches to an Old Problem: Original Techniques in [FeFe]-hydrogenase Research
... of hydrogenase biodiversity. Moving forward, investigating novel [FeFe]-hydrogenases will help understanding the molecular proceedings of hydrogen ...
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Investigation of the ultrafast dynamics occurring during unsensitized photocatalytic H2 evolution by an [FeFe]-hydrogenase subsite analogue
... of hydrogenase-based biomimetic compounds are limited and tend toward excitation at wave- lengths shorter than 400 nm but, in all of these cases, car- bonyl ligand photolysis has been a predominant outcome and it ...
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Elimination of the flavodiiron electron sink facilitates long-term H2 photoproduction in green algae
... became clear that unlike in S-deprivation, under magne- sium deprivation conditions both PSII and FDPs remain present for a longer period [41]. In line with this observa- tion, it was proposed that FDPs are presumably ...
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Encapsulating Subsite analogues of the [FeFe]-hydrogenases in micelles enables direct water interactions
... Encapsulation of sub-site analogues of the [FeFe]-hydrogenase enzymes in supramolecular structures has been shown to dramatically increase their catalytic ability, but the molecular basis for this ...
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Detection of transient intermediates generated from subsite analogues of [FeFe]-hydrogenases
... This article reviews the application of transient techniques in the elucidation of electron, proton and photon chemistry related to the catalytic subsite of [FeFe]-hydrogenase from the perspective of ...
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The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance
... Hydrogen gas production occurs in certain microor- ganisms that catalyze its production through two enzyme families: nitrogenases that fix atmospheric nitrogen into ammonia while emitting gaseous hydrogen as a byprod- ...
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Advances in Utilizing Cyanobacteria for Hydrogen Production
... than energetically more favorable Fd as the preferred electron donor (Figure 1). Therefore, heterologous ex- pression of highly active enzymes from other organisms is explored. Recent studies show that expression of ...
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Hydrogenases and Hydrogen Sensors in the Symbiotic Microbial Communities of Wood Feeding Termites
... Influence of host feeding habits. Unifrac principle component and jackknife clustering analyses of a maximum likelihood tree of all higher termite sequences, see Figures 4-6, revealed a close clustering of the Amitermes ...
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Sawyeria marylandensis (Heterolobosea) Has a Hydrogenosome with Novel Metabolic Properties
... three hydrogenase naturase proteins, and a single gene for pyruvate: ferredoxin oxidoreductase (PFO) that, where known, possess an N-terminal mitochondrial/hydrogenosomal targeting pep- tide (HydE is a partial ...
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On the pathways feeding the H2 production process in nutrient-replete, hypoxic conditions. Commentary on the article “Low oxygen levels contribute to improve photohydrogen production in mixotrophic non-stressed Chlamydomonas cultures”, by Jurado-Oller et al., Biotechnology for Biofuels, published September 7, 2015; 8:149
... (reviewed in [1]). Under anaerobic conditions, chloro- plast-located [FeFe]-hydrogenase enzymes (HYDA1 and HYDA2) function as acceptors for electrons derived mostly from the photosynthetic electron ...
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Extracellular electron transfer from cathode to microbes: application for biofuel production
... The study of enzyme purification and protein iden- tification using mass spectroscopy in an acetotrophic methanogen, Methanosarcina acetivorans, showed that ferredoxin reduced membrane-associated multi-heme cytochrome c ...
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An analysis of the changes in soluble hydrogenase and global gene expression in Cupriavidus necator (Ralstonia eutropha) H16 grown in heterotrophic diauxic batch culture
... of hydrogenase gene expression upon fructose depletion was confirmed in replicate ...with hydrogenase expression, significant changes were observed in genes associated with energy transduction, amino acid ...
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Improved production of biohydrogen in light-powered Escherichia coli by co-expression of proteorhodopsin and heterologous hydrogenase
... E. coli is a chemotroph that cannot utilize light energy. Bacteriorhodopsins, including proteorhodopsin, are the simplest molecules that enable microorganisms to uti- lize solar energy to create a proton gradient and ...
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Cloning, Disruption and Chromosomal Mapping of Yeast LEU3, a Putative Regulatory Gene
... Finally, double mutants containing leucine-re- sistant a-IPM synthase and lacking isomerase or de- hydrogenase activity produce the remaining activity at levels that [r] ...
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In vitro Anti-adenovirus activity of pomegranate (Punica granatum L.) peel extract
... supplements with antioxidant activity. 1,5,9,10 Basically, natural supplements have less harms than artificial supplements & against artificial supplements. Aloe Vera is rich in vitamin C and E with antioxidant ...
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Conserved Histidine Adjacent to the Proximal Cluster Tunes the Anaerobic Reductive Activation of Escherichia coli Membrane-Bound [NiFe] Hydrogenase-1
... The procedure for the successful generation of variants in which His-229 was replaced with Ala in E. coli Hyd-1 and His- 214 was replaced with Ala in E. coli Hyd-2 is described in SI. The purification of these variants ...
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Distal [FeS]-Cluster Coordination in [NiFe]-Hydrogenase Facilitates Intermolecular Electron Transfer
... coordination. Clearly, it is not the reorganization energy that is causing the difference in ET rates. The different electronic coupling between the amino acid histidine with its π-electrons and the aromatic amino acid ...
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A Study of Potential Toxic Effects After Repeated 10-Week Administration of a New Iron Chelator – Salicylaldehyde Isonicotinoyl Hydrazone (SIH) to Rabbits
... hydrogenase (LD), and glutathione peroxidase (GSH-px) at the end of the experiment in comparison with the initial values (Table 1). However, the initial value of calcium con- centration was significantly higher in ...
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Recovery of platinum(0) through the reduction of platinum ions by hydrogenase-displaying yeast
... of hydrogenase on yeast cell membrane, immunofluorescent labeling of cells with or without cell wall digestion was performed using the anti- body against the FLAG epitope tag as described previ- ously (Nishitani ...
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Pyridoxine-dependent Epilepsy With Elevated Urinary α-Amino Adipic Semialdehyde in Molybdenum Cofactor Deficiency
... The assessment of urinary a -amino adipic semialdehyde ( a -AASA) has be- come the gold standard to evaluate the biochemical diagnosis of a -AASA de- hydrogenase de fi ciency (MIM 107323)[r] ...
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