Fetal bovine serum
Rotavirus specific antibodies in fetal bovine serum and commercial preparations of serum albumin
... Rotavirus-specific antibodies were detected in fetal bovine serum, bovine serum albumin, and human serum albumin by radioimmunoprecipitation with the NCDV strain of bovine rotavirus as t[r] ...
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Fetal bovine serum: geographic origin and regulatory relevance of viral contamination
... of fetal bovine serum (FBS), who assume that cer- tain countries, like Australia and New Zealand, have fewer cattle disease viruses and pose less risk for the presence of viruses, than do the other ...
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Genetic and epigenetic instability of human bone marrow mesenchymal stem cells expanded in autologous serum or fetal bovine serum
... Fetal bovine serum (FBS) remains to date the primary source of growth supplements and low molecular weight bioactive compounds for long-term in vitro expansion of MSCs (Kume et ...AB serum ...
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Activity in fetal bovine serum that stimulates erythroid colony formation in fetal mouse livers is insulinlike growth factor I
... of fetal serum was characterized. Using fetal bovine serum (FBS) as a source of the erythropoietic activity and serum-free cultures of fetal mouse livers (FMLC assay) as a ...
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Modification of the 5′ Terminus of Sindbis Virus Genomic RNA Allows nsP4 RNA Polymerases with Nonaromatic Amino Acids at the N Terminus To Function in RNA Replication
... Secondary chicken embryo fibroblast monolayer cells cultured in Eagle’s minimum essential medium supplemented with 3% fetal bovine serum 2301 Downloaded from http://jvi.asm.org/ on Novem[r] ...
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The effect of autologous adipose derived mesenchymal stem cell therapy in the treatment of a large osteochondral defect of the knee following unsuccessful surgical intervention of osteochondritis dissecans – a case study
... Abbreviations BSC: Biosafety cabinet; FACS: Florescent Activated Cell Sorting; FBS: Fetal Bovine Serum; ICRS: International Cartilage Repair Society; KOOS: Knee Injury and Osteoarthritis[r] ...
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Proteasome inhibitors act as bifunctional antagonists of human immunodeficiency virus type 1 latency and replication
... ELISA: Enzyme-linked immunosorbent assay; Env: Envelope; FBS: Fetal bovine serum; FDA: Food and drug administration; FITC: Fluorescein isothiocyanate; GAPDH: Glyceraldehyde 3-phosphate d[r] ...
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Human umbilical cord Wharton jelly cells promote extra-pancreatic insulin formation and repair of renal damage in STZ-induced diabetic mice
... Isolation, culture and characterization of hUCWJCs The protocols for sampling human umbilical cord tissue were approved by the Institutional Review Board of Shantou University Medical College. All participants pro- vided ...
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Targeting intracellular MMPs efficiently inhibits tumor metastasis and angiogenesis
... PTX with more than 98% purity was purchased from Yew Biotechnology Co. Ltd. (Jiangsu, China). Taxol (marked product of PTX) was purchased from Bristol-Myers Squibb (China) Investment Co. Ltd. (Shanghai, China). β-CN (No. ...
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Neuroprotective effects of vitamin D on high fat diet- and palmitic acid-induced enteric neuronal loss in mice
... C57Bl/6J mice (n=46, 20-23g, Janvier Labs, FR) on standard chow diet (R36 Lactamin, SE) were used for in vitro experimentations, animals were housed in groups. Primary cultures of myenteric neurons were prepared from the ...
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Type I collagen facilitates safe and reliable expansion of human dental pulp stem cells in xenogeneic serum-free culture
... xenogeneic serum, i.e., fetal bovine serum (FBS), is necessary for MSC cul- ture to isolate and expand donor-derived stem/stromal cells, including DPSCs [23, ...However, serum from an ...
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The role of microglia and the TLR4 pathway in neuronal apoptosis and vasospasm after subarachnoid hemorrhage
... FBS: Fetal bovine serum; ICV: Intracerebroventricular; IRAK4: IL-1 receptor associated kinase 4; LPS: Lipopolysaccharide; MCA: Middle cerebral artery; MCSF: Macrophage colony-stimulating factor; ...
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A Chinese herbal formula "Gan Lu Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase 2/9 through the PI3K/AKT and ERK signaling pathways
... Dulbecco’s Modified Eagle’s Medium (DMEM), 3-(4, 5- dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT), RNase A, and other chemicals were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Trypsin - EDTA, ...
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An investigation into the effects of magnetic fields on biological cell processes
... field Electron paramagnetic resonance Extracellular-signal-regulated kinase Electron transport chain Fetal bovine serum Finite element method Glutathione Glutathione peroxidase High cont[r] ...
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The effect of chlormadinone acetate on odontogenic differentiation of human dental pulp cells: in vitro study
... FBS: Fetal bovine serum; GM: Growth media; hBMSC: Human bone marrow-derived mesenchymal stem cells; hDPC: Human dental pulp cell; HRP: Horseradish peroxidase; IRB: Institutional review board; JNK: ...
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Nanogel-crosslinked nanoparticles increase the inhibitory effects of W9 synthetic peptide on bone loss in a murine bone resorption model
... 10% serum was enough to induce W9 peptide release from the CHP nanogels, but not from the NanoClik nanoparticles, indicating that the NanoClik nanoparticles were essential for the long-term controlled release of ...
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The omega-3 polyunsaturated fatty acid docosahexaenoic acid inhibits proliferation and progression of non-small cell lung cancer cells through the reactive oxygen species-mediated inactivation of the PI3K /Akt pathway
... FBS: Fetal bovine serum; FITC: Fluorescein isothiocyanate; FO: Dietary fish oil; HRP: Horseradish peroxidase; MMP9: Matrix metallopeptidase; MTT: 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl ...
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The Apoptosis Effect on Liver Cancer Cells of Gold Nanoparticles Modified with Lithocholic Acid
... Sigma-Aldrich (St. Louis, MO) and used without fur- ther purification. RPMI-1640 media and fetal bovine serum (FBS) were from Invitrogen Corporation. HepG2 (human hepatocellular liver carcinoma ...
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Peripheral Blood Lymphocyte Culture of Rhinella arenarum
... penicillin-streptomycin, fetal bovine serum and ...1640; fetal bovine serum: he worked with a volume of 500 μl in the case of RPMI 1640 and F10, for MEM 1000 μl was used; ...
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Serum decreases the size of Metafectene-and Genejammer-DNA complexes but does not affect significantly their transfection activity in SCCVII murine squamous cell carcinoma cells
... in serum-free ...mouse serum (up to ...mouse serum more accurately mimics human serum conditions compared to FBS [38, ...mouse serum to DOTAP:Chol/DNA or DOTAP:DOPE/DNA lipoplexes, at a ...
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