Fiber-modified adenovirus vectors
Selective targeting of human cells by a chimeric adenovirus vector containing a modified fiber protein.
... The adenovirus fiber protein is responsible for attachment of the virion to unidentified cell surface ...distinct adenovirus fiber receptors which interact with the group B (Ad3) and group C ...
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Generation of recombinant adenovirus vectors with modified fibers for altering viral tropism.
... the adenovirus to allow interaction with cellular receptors in a specific ...the fiber protein (29, 36, 54) which protrude from each of the 12 vertices of the icosahedral ...the adenovirus ...
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Fiber Swap between Adenovirus Subgroups B and C Alters Intracellular Trafficking of Adenovirus Gene Transfer Vectors
... Ad7 vectors with respect to transgene expression as assessed by CAT assay 12 to 72 h after infection (1, ...Ad7 fiber protein, therefore, do not affect the ultimate efficacy of the vector over ...of ...
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Original Article A fiber-modified adenovirus co-expressing HSV-TK and Coli.NTR enhances antitumor activities in breast cancer cells
... Due to both high levels of CAR and large endo- thelial pores to allow Ad vectors easy access to the liver, unwanted foreign gene transfer into this organ is a concern [10]. We therefore per- formed ...
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Increased in vitro and in vivo gene transfer by adenovirus vectors containing chimeric fiber proteins.
... two modified vectors were not dramatically af- fected by the addition of ligands onto the end of the fiber ...the modified vectors were compared on different cell types ...the ...
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Contrasting Effects of Human, Canine, and Hybrid Adenovirus Vectors on the Phenotypical and Functional Maturation of Human Dendritic Cells: Implications for Clinical Efficacy
... viral vectors are delivered in substantial doses and are incapable of expressing gene products that reduce the host’s ability to detect transduced ...also modified by the in vivo transduction of dendritic ...
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Tropism Modification of Adenovirus Vectors by Peptide Ligand Insertion into Various Positions of the Adenovirus Serotype 41 Short-Fiber Knob Domain
... recombinant fiber molecules containing the Ad41s fiber knob with the RGD peptide into virus ...recombinant fiber molecules into virus particles and to investigate viral transduction mediated by the ...
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RGD Inclusion in the Hexon Monomer Provides Adenovirus Type 5-Based Vectors with a Fiber Knob-Independent Pathway for Infection
... the fiber knob (11–13, 23, ...the fiber C terminus increases transduction both in vitro and in vivo, most probably because virus inter- nalization occurred after virus attachment to heparan sulfate ...
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Evaluation of a Fiber-Modified Adenovirus Vector Vaccine against Foot-and-Mouth Disease in Cattle
... rAd vectors improve the transduction efficiency of integrin-rich cell types such as endothelial cells, smooth muscle cells, and DCs (25, 28, ...RGD-modified fiber of the ...(RGD- ...
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Adenovirus Vector Pseudotyping in Fiber-Expressing Cell Lines: Improved Transduction of Epstein-Barr Virus-Transformed B Cells
... While adenovirus (Ad) gene delivery vectors are useful in many gene therapy applications, their broad tropism means that they cannot be directed to a specific target ...Ad vectors, such as ...
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Hexon Hypervariable Region-Modified Adenovirus Type 5 (Ad5) Vectors Display Reduced Hepatotoxicity but Induce T Lymphocyte Phenotypes Similar to Ad5 Vectors
... Ad vectors can differ markedly in their innate and adaptive immunological properties and that both the Ad fiber and capsid proteins influence these properties (4, 8, 21, 27, ...Ad vectors. Our data ...
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Frequency and Stability of Chromosomal Integration of Adenovirus Vectors
... DE1 vectors, the level of b-Gal activity decreased from high to low over time (clones 2 and 4) ...type adenovirus, which is often shut off by methylation ...
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Improved Adenovirus Vectors for Infection of Cardiovascular Tissues
... improved adenovirus vectors for cardiovascular gene therapy, a library of adenovirus vectors based on adenovirus serotype 5 (Ad5) but carrying fiber molecules of other human ...
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Tumor-Specific, Replication-Competent Adenovirus Vectors Overexpressing the Adenovirus Death Protein
... the vectors will destroy noncancerous proliferating cells in the ...The vectors rep- licated in HEL-299 and WI-38 cells growing in 10% serum, but it is unlikely that these cells are truly ...the ...
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Transduction of the Mammary Epithelium with Adenovirus Vectors In Vivo
... Extent of adenovirus transduction. We viewed transduced mammary glands at low magnification to obtain an initial as- sessment of the amount of Ad5GFP transduction (Fig. 2B, upper panel, and 2C). Although ...
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Association of the Human Papillomavirus Type 11 E1 Protein with Histone H1
... the fiber protein ...The adenovirus fiber protein was expressed in HeLa cells from vaccinia ...the fiber-expressing cells were formed by using an antifiber or anti-H1 antibody, and the ...
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Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy.
... enumerating adenovirus vector virions by bi- ological assays can be divided roughly into intrinsic properties of the virion and events which occur during the evaluation process itself as described in equations 8 ...
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Construction of adenovirus vectors through Cre-lox recombination.
... Gutless virus construction. Since the selection against C5 functions in cis, it should be a good helper virus. Therefore, we decided to use C5 to complement growth of an adenovirus vector which was missing all ...
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Detection of Antibodies against Adenovirus Protein IX, Fiber, and Hexon in Human Sera by Immunoblot Assay
... of adenovirus antibodies with an immunoblot assay using recombinant pIX and fiber proteins had not been described until ...detect adenovirus antibodies in a species-specific manner, we analyzed at ...
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Characterization of human adenovirus 35 and derivation of complex vectors
... rAd35 vectors a triple- deleted vector was ...rAd35 vectors with this combination of deletions yielded 36,000 pu/cell, which is comparable to the E1-deleted rAd35 (Table ...competent adenovirus (RCA) ...
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