Fibroblast cultures
Demonstration of elastin gene expression in human skin fibroblast cultures and reduced tropoelastin production by cells from a patient with atrophoderma
... biosynthesis in most tissues is under pretranslational control, molecular hybridization to a nick-translated genomic elastin probe was performed; however, elastin messenger RNA levels were equivalent in both cell ...
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Enhanced biosynthesis of human skin collagenase in fibroblast cultures from recessive dystrophic epidermolysis bullosa
... in fibroblast cultures from patients with recessive dystrophic epidermolysis ...control cultures. Recessive dystrophic epidermolysis bullosa cultures accumulated increased amounts of ...mutant ...
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Effect of Interferon on Deoxyribonuclease Induction in Chick Fibroblast Cultures Infected with Cowpox Virus
... 466-471 Copyright @ 1967 American Society for Microbiology Effect of Interferon on Deoxyribonuclease Induction in Chick Fibroblast Cultures Infected with Cowpox Virus Received for public[r] ...
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Proliferation and the action of an anti-proliferative agent in Dupuytren's fibroblast cultures
... A concentration of 2 ng/mL TGFpi for 5 days was seen to significantly increase the myofibroblast differentiation in non-diseased fascia and carpal ligament cultures for two days only, but caused a sustained ...
190
Altered intercellular communication in lung fibroblast cultures from patients with idiopathic pulmonary fibrosis
... lung fibroblast cultures (N1-5) and 3 fibrotic fibroblast cul- tures ...normal fibroblast lines and 3 fibrotic fibrob- last ...lung fibroblast, FF fibrotic lung fibrob- ...
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Correlation between the radiation responses of fibroblasts cultured from individual patients and the risk of late reaction after breast radiotherapy.
... irradiation of fibroblasts established from unirradiated skin was significantly increased over the background. The median for all patients was 2.07 [quartiles: 1.73; 2.46] for 4 Gy versus 0.64 [quartiles: 0.51; 0.83] for ...
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Collagen gene expression by cultured human skin fibroblasts Abundant steady state levels of type VI procollagen messenger RNAs
... demonstrated the presence of specific mRNA transcripts for collagen types I, III, IV, V, and VI, but not for type II collagen. Quantitation of the abundance of these mRNAs by slot blot hybridizations revealed that type ...
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n Butyrate inhibition of hyaluronate synthesis in cultured human fibroblasts
... Normal fibroblast cultures were grown to confluence, shifted to a medium without or with n-butyrate for 24 h, labeled with either [3H]acetate or [3H]glucosamine and analyzed for [3H]GAG ...
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Persistence of a reduced collagen producing phenotype in cultured scleroderma fibroblasts after short term exposure to interferons
... inflammation-associated, fibroblast-stimulatory factors appears to initiate fibrosis by inducing the persistently activated phenotypes displayed by fibroblast cultures derived from scleroderma skin ...
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Biotin response Organicaciduria: MULTIPLE CARBOXYLASE DEFECTS AND COMPLEMENTATION STUDIES WITH PROPIONICACIDEMIA IN CULTURED FIBROBLASTS
... Fibroblast cultures from two individuals with biotin-responsive organicacidemia were found to have a pleiotropic deficiency of propionyl-CoA carboxylase, b-methylcrotonyl-CoA carboxylase[r] ...
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Increased glycosaminoglycan accumulation as a genetic characteristic in cell cultures of one variety of dominant dystrophic epidermolysis bullosa
... Fibroblast cultures from patients with dominant dystrophic epidermolysis bullosa of the albopapuloid variety display deranged glycosaminoglycan metabolism. These cells accumulate increased amounts of ...
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A human neuronal model of Niemann Pick C disease developed from stem cells isolated from patient’s skin
... Additional file 3: Figure S2. Hepatic differentiation of hSKIN-MASC obtained from already established skin fibroblast cultures. (A-B) Phase contrast images of healthy donor- (A) and NPC patient- (B) derived ...
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HPV-negative, but not HPV-positive, oropharyngeal carcinomas induce fibroblasts to support tumour invasion through micro-environmental release of HGF and IL-6.
... Fibroblast cultures stimulated by HPV-negative OPC cell lines produced conditioned media that was supportive of cancer cell migration when re-exposed to the respective stimulating carcinoma cell ...
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Demonstration of the Heterozygous State in Hunter's Syndrome
... sulfate uptakes in fibroblast cultures from normal. individuals when medium pH was maintained[r] ...
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BDNF/TrkB axis activation promotes epithelial–mesenchymal transition in idiopathic pulmonary fibrosis
... IPF fibroblast cell line were seeded in 60 mm Petri dishes at density of 2 × 105 cells in RPMI medium sup- plemented with 5% ...IPF fibroblast cell line were evaluated after 24, 48 and 120 ...
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Isolation of Coxiella burnetii from heart valves of patients treated for Q fever endocarditis
... Acute infection with C. burnetii may proceed to a chronic disease, mainly chronic endocarditis or granulomatous hepati- tis (19). However, the frequency of this event is not known. Since the first descriptions of Q fever ...
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Role of the Nuclease Activities Encoded by Herpes Simplex Virus 1 UL12 in Viral Replication and Neurovirulence
... cell cultures, based on reports showing that (i) UL12 null mutant virus exhib- ited a 100- to 1,000-fold reduction in progeny virus titer in cell cultures (4, 5) and (ii) wild-type UL12 efficiently ...
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Ecotropic murine leukemia virus-induced fusion of murine cells.
... Cell lines used in this study Cell line B559 Mouse fibroblast Mouse, transformed fibroblast Mouse fibroblast Mouse fibroblast Mouse fibroblast Mouse fibroblast Mouse fibroblast Mouse fib[r] ...
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Genomics and proteomics approaches to the study of cancer-stroma interactions
... Fibroblasts, as other cells in tumor microenvironments, need to maintain close communication with cancer cells, promoting proliferation, recruitment of inflamma- tory cells and acquisition of invasive characteristics. ...
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Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae): a novel model to assay Leishmania spp and vector interaction
... in cultures of human macrophage cells [5,6]; J774 (murine) macrophages [7,8]; fibroblast cell lines [9,10], epithelial cells [11], dendritic cells [12,13], neutrophil granulocytes [14]; Aedes albopictus ...
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