fibronectin-binding
Surface displaced alfa-enolase of Lactobacillus plantarum is a fibronectin binding protein
... detect fibronectin binding, overlay assays were performed as described [28] with some modifica- tions: the PVDF membranes were incubated with blocking buffers I and II, washed with NN' buffer and overlaid ...
10
Staphylococcus aureus Fibronectin Binding Protein A Mediates Cell Cell Adhesion through Low Affinity Homophilic Bonds
... surface-located fibronectin-binding protein A (FnBPA) plays an important role in the accumulation phase of biofilm formation by methicillin-resistant ...Low-affinity binding by means of FnBPA may be ...
10
Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin binding adhesins fibronectin binding protein A and fibronectin binding protein B from Staphylococcus aureus
... weakly. Fibronectin-binding protein B has been postulated to harbor 10 rather than 11 repeats, but it contains the same number of high- affinity fibronectin-binding sites as fibronectin-binding ...
16
Sequence diversity in the A domain of Staphylococcus aureus fibronectin binding protein A
... Schematic representation of FnBPA from S. aureus 8325-4. (a) The N-terminus of FnBPA contains a signal sequence (S) followed by a 475 amino acid A domain, part of which is the binding site for fibrinogen and ...
14
Fibronectin Binding Protein Gene Recombination and Horizontal Transfer between Group A and G Streptococci
... Partial sequence from the variable 5 ⬘ end of gfbA (Fig. 2A) and the published gfbA sequence (accession number U31115) (17) were compared with previously reported sfbI sequences from GAS. Overall, the architectural ...
5
The Borrelial Fibronectin-Binding Protein RevA Is an Early Antigen of Human Lyme Disease
... Previous studies using small numbers of serum samples from human patients and experimentally infected animals identified the frequent presence of antibodies recognizing RevA, a borrelial fibronectin- ...
7
Fibronectin binding protein B variation in Staphylococcus aureus
... Ligand binding by recombinant FnBPB N23 isotypes was compared by ELISA-based solid phase assays and surface plasmon ...and fibronectin dose-dependently and saturably with similar ...affinities. ...
37
A novel Staphylococcus aureus biofilm phenotype mediated by the fibronectin binding proteins, FnBPA and FnBPB
... The fibronectin binding proteins A and B (FnBPA and FnBPB) are multifunctional LPXTG-anchored surface pro- teins that comprise two distinct ligand binding domains (63) ...of fibronectin ...
17
Specific Identification of Listeria welshimeri and Listeria monocytogenes by PCR Assays Targeting a Gene Encoding a Fibronectin Binding Protein
... human fibronectin, as previously described ...human fibronectin (lanes 2 to 4), whereas the 26-kDa GST produced by ...bind fibronectin (arrow, lane 1). This indicates that the binding of ...
6
Subdomains N2N3 of Fibronectin Binding Protein A Mediate Staphylococcus aureus Biofilm Formation and Adherence to Fibrinogen Using Distinct Mechanisms
... surface-located fibronectin binding proteins A and B (FnBPA, FnBPB). Here we provide new insights into the requirements for FnBP-dependent biofilm formation by MRSA. We show that expression of FnBPs is ...
9
Threonine-Rich Repeats Increase Fibronectin Binding in the Candida albicans Adhesin Als5p
... in binding of yeast cells to a variety of substrates, such as extracellular matrix-coated beads, human buccal epithelial cells, and various peptide sequences (9, 10, 22, 28, 32, ...initial binding of ...
10
The role of fibronectin binding in the rat model of experimental endocarditis caused by Streptococcus sanguis
... of fibronectin (Fn) binding by insertional mutagenesis of Streptococcus sanguis with Tn916 reduces virulence of this bacterium in the rat model of infective endocarditis ...
8
Human Immunoglobulin G Cannot Inhibit Fibrinogen Binding by the Genetically Diverse A Domain of Staphylococcus aureus Fibronectin-Binding Protein A
... ABSTRACT The fibronectin-binding protein A (FnBPA) is a cell surface-associated protein of Staphylococcus aureus which mediates adherence to the host extracellular matrix and is important for bacterial virulence. ...
11
Fibrinogen activates the capture of human plasminogen by staphylococcal fibronectin binding proteins
... analyze binding of Fg to immobilized Plg, microtiter plate wells were coated with 200 ng Plg, blocked with BSA, and incubated with increasing amounts of human ...N2N3 binding, microtiter plates were coated ...
12
Adaptation in a Fibronectin Binding Autolysin of Staphylococcus saprophyticus
... Invasion of the human urinary tract may provide a fitness advantage by allowing relative enrichment of S. saprophyticus in a site with little competition from other bacterial species and by providing a mechanism of ...
17
Fibrinogen Activates the Capture of Human Plasminogen by Staphylococcal Fibronectin Binding Proteins
... analyze binding of Fg to immobilized Plg, microtiter plate wells were coated with 200 ng Plg, blocked with BSA, and incubated with increasing amounts of human ...N2N3 binding, microtiter plates were coated ...
12
Fibrinogen and Fibronectin Binding Activity and Immunogenic Nature of Choline Binding Protein M
... The results of DNA sequencing showed that the cbpA coding locus exists in all tested virulent strains of S. pneumoniae. However, the locus en- codes CBPs with allelic variation in the anchor region, which results in ...
8
Fibronectin binding by Salmonella strains: evaluation of a particle agglutination assay
... Thirty-five Salmonella strains isolated from human cases of salmonellosis were tested and compared for their fibronectin fn binding capacities by using two fn-particle agglutination assa[r] ...
7
A short sequence within subdomain N1 of region A of the Staphylococcus aureus MSCRAMM clumping factor A is required for export and surface display
... export or cell wall localization of FnBPB lacking the flexible fibronectin binding repeat... Clumping factor A is the archetypal member of the MSCRAMM family of wall-.[r] ...
41
Reduced surface expression and binding of fibronectin by thrombin stimulated thrombasthenic platelets
... stimulated fibronectin binding to coincubated normal cells, suggesting that their defect was not due to the presence of a soluble inhibitor of fibronectin ...of fibronectin to normal cells, ...
7