FISH and 16S rRNA gene-based analyses
Phylogenetic analyses of Chlamydia psittaci strains from birds based on 16S rRNA gene sequence
... in rRNA sequences showed less than 50% relatedness in DNA reassociation (1, ...homologous 16S rDNAs do not always reflect genealogically close ...tree based on the 16S rDNA sequence were more ...
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reaction and 16S-rRNA gene sequences
... areas based on repetitive sequence-based polymerase chain reaction and 16S- rRNA gene ...with 16S-rRNA gene sequences followed by cluster analysis using the results ...
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Identification of Bacterial Fish Pathogens in Brazil by Direct Colony PCR and 16S rRNA Gene Sequencing
... Intensive fish farming systems in Brazil have increased the disease incidence, mainly of bacterial origin, due to higher stocking density, high organic matter levels and poor quality of the aquatic environment ...
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Deciphering chicken gut microbial dynamics based on high-throughput 16S rRNA metagenomics analyses
... Mohd Asrore Mohd Shaufi 1 , Chin Chin Sieo 1,2* , Chun Wie Chong 3 , Han Ming Gan 4 and Yin Wan Ho 1 Abstract Background: Chicken gut microbiota has paramount roles in host performance, health and immunity. Understanding ...
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Deciphering chicken gut microbial dynamics based on high-throughput 16S rRNA metagenomics analyses
... Mohd Asrore Mohd Shaufi 1 , Chin Chin Sieo 1,2* , Chun Wie Chong 3 , Han Ming Gan 4 and Yin Wan Ho 1 Abstract Background: Chicken gut microbiota has paramount roles in host performance, health and immunity. Understanding ...
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Detection of Mycoplasma genitalium by PCR Amplification of the 16S rRNA Gene
... genitalium 16S rRNA gene PCR showed discrepant results in the MgPa- 476–MgPa-903 assay indicates that the decreased clinical sen- sitivity could be due to a strain-dependent relative lack of ...
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Species Specific Identification of Leptospiraceae by 16S rRNA Gene Sequencing
... genomospecies based upon DNA-DNA reassociation ...leptospires. 16S rRNA gene sequences are potentially useful for species identification of Leptospira, but there are a large number of ...
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Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians
... phenetic analyses (Bayesian and Neigh- bor-joining) of these and many additional sequences (to be published elsewhere) mostly grouped sequences of those specimens from Ranomafana and Andasibe that a priori had ...
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Identification of Pathogenic Nocardia Species by Reverse Line Blot Hybridization Targeting the 16S rRNA and 16S 23S rRNA Gene Spacer Regions
... 16S rRNA gene-based probes correctly identified 124 of 135 isolates (sensitivity, 92%) but were unable to identify Nocardia paucivorans strains (n ⴝ 10 strains) and a Nocardia asteroides ...
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Identification by 16S rRNA Gene Analyses of a Potential Novel Mycobacterial Species as an Etiological Agent of Canine Leproid Granuloma Syndrome
... the 16S rRNA gene were performed on 46 specimens obtained from 43 dogs with canine leproid granuloma syndrome to help determine its ...Molecular analyses of the paraffin-embedded tissues and ...
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C16S — A Hidden Markov Model based algorithm for taxonomic classification of 16S rRNA gene sequences
... BLAST based approach, several metagenomic studies use the RDP classi fi er for taxonomically characterizing 16S rDNA frag- ments ...BLAST based approach, the RDP classi fi er fi rst identi fi es a ...
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Determination of the etiology of presumptive feline leprosy by 16S rRNA gene analysis
... by 16S rRNA gene ...the 16S rRNA primers used are not strictly universal and would not amplify all bacterial ...biology-based analyses; this probably reflects the ...
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Detection of Kanamycin ResistantMycobacterium tuberculosis by Identifying Mutations in the 16S rRNA Gene
... the 16S rRNA gene and 38 bp of the intervening sequence between the 16S and the 23S rRNA genes were made by PCR and were used for PCR-based direct ...
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Catheter Related Microbacterium Bacteremia Identified by 16S rRNA Gene Sequencing
... First, the descriptions of many Microbacterium species are based on a single strain or very few strains. Therefore, pheno- typic species identification is almost impossible, and it is un- likely that there will be ...
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Species Specific Identification of Campylobacters by Partial 16S rRNA Gene Sequencing
... strains. 16S rRNA gene (16S rDNA)-based identification of bacteria offers a possible alternative when phenotypic tests ...of 16S rDNA sequencing for the species-specific ...
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Controlling for Contaminants in Low-Biomass 16S rRNA Gene Sequencing Experiments
... as 16S rRNA gene ...polymicrobial 16S rRNA gene sequencing experiments, we created a mock microbial community dilution ...sequences based on relative abundance, (iii) ...
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A comprehensive evaluation of the sl1p pipeline for 16S rRNA gene sequencing analysis
... chosen based on the analyses presented within this ...database based on its superior performance in the Bray-Curtis distance comparisons of the HMP-mock communities and as one of the best choices for ...
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DETECTION OF BACTERIA BY AMPLIFYING THE 16S rRNA GENE WITH UNIVERSAL PRIMERS AND RFLP
... The 16S rRNA genes of almost all common bacterial pathogens found in body fluids have been sequenced,2,8,16 With one set of designed universal primers that can amplify this gene in all bacte ria ...
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Tax4Fun2: prediction of habitat-specific functional profiles and functional redundancy based on 16S rRNA gene sequences
... might change between different environments. None- theless, a contemporary concern for the conservation of biodiversity and the development of management strat- egies is that decision makers require quantitative mea- ...
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Denitrification potential of the eastern oyster microbiome using a 16S rRNA gene based metabolic inference approach
... other gene-based metabolic inference analyses, limitations exist regarding the quality and scope of the reference database being used as well as the understanding of the gene and metabolic ...
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