FL, FITC
Mixed Micellar Electrokinetic Chromatographic Analysis of Colistin, Polypeptide Antibiotic, Using Laser Induced Fluorescence Detection
... between FITC and colistin was investigated by comparison of the peak areas of colistin-FITC derivatives at various time ...The FITC-colistin derivative was formed within 10 min, but maximum yield was ...
9
<p>Cationic/Anionic Polyelectrolyte (PLL/PGA) Coated Vesicular Phospholipid Gels (VPGs) Loaded with Cytarabine for Sustained Release and Anti-glioma Effects</p>
... Cytarabine (purity 99.6%, MW 279.68) was purchased from Peking University Pharmaceutical Co., Ltd. (Beijing, China). Egg lecithin (E80) was obtained from Lipoid GmbH (Ludwigshafen, Germany). Sodium Cholesteryl sulfate ...
12
Investigation of folate-conjugated fluorescent silica nanoparticles for targeting delivery to folate receptor-positive tumors and their internalization mechanism
... The quantitative analysis and evaluation of NPs cellular internalization were performed by flow cytometry, the results of which are shown in Figure 9. Histograms showing the cellular internalization of A549 and KB cells ...
10
Blood–brain barrier transport studies, aggregation, and molecular dynamics simulation of multiwalled carbon nanotube functionalized with fluorescein isothiocyanate
... FITC fluorescence was measured after 2, 24, and 48 hours by the Tecan GENios Microplate Reader (MTX Lab Systems, Inc., Vienna, VA, USA). Subsequently, the cEND cells were fixed with 2% of paraformaldehyde for ...
11
Hepatocellular carcinoma-targeted effect of configurations and groups of glycyrrhetinic acid by evaluation of its derivative-modified liposomes
... with FITC-GA samples and the only FITC-GA samples normalized with the content of protein in the cells was calculated as the specific binding (B) and maximum binding (B 0 ), ...
12
An Indirect Immunoassay for Detecting Antigen Based on Fluorescence Resonance Energy Transfer
... with FITC (1 mg/mL) in ...anti-BSA– FITC were measured. The stoichiometry for the binding of FITC to anti-BSA antibody was obtained by the UV-Vis absorption peak of various concentration of an- ...
7
Extracellular cyclophilin A possesses chemotaxic activity in cattle
... primers (sense, 5′-TAGCTCAAGCTTTGATGGTCAACC CCACCGTGTTCTTC-3′; antisense, 5′-CGCTCGCTCGA GTTAGATTTGTCCACAGTCAGCAAT-3′; HindIII and XhoI restriction enzyme sites are shown by underlining). PCR fragment and the expression ...
10
Single step assembly of polymer lipid hybrid nanoparticles for mitomycin C delivery
... It should be noted that, the fluorescence signals of H22 cells incubated with the PLA NPs/FITC-MMC-SPC were significantly increased compared with those incubated with the PLA NPs/FITC-MM[r] ...
14
Niosomes and polymeric chitosan based vesicles bearing transferrin and glucose ligands for drug targeting
... Cellular FITC-dextran uptake was examined on a FACStar flow cy- tometer (Becton-Dickinson Instruments, ...sample. FITC−dextran fluorescence was detected with logarithmic settings (FL1, em ⳱ 515–545 ...
9
Alig, Annabel H. S. (2018): Untersuchungen zum Freisetzungsmechanismus neuartiger, auf Phosphatidyldiglycerin-basierender sowie pegylierter, Lysolipid-haltiger thermosensitiver Liposomen und deren Schicksal in Zellkultur. Dissertation, LMU München: Medizinische Fakultät
... Freisetzung kleiner Moleküle wie CF. Für das größere Molekül FITC-Dextran bildeten sie jedoch eine Barriere. Eine mögliche Erklärung dieses Freisetzungsmechanismus wären „grain boundaries“, die bei einer ...
109
Adrenomedullin increases fibroblast like synoviocyte adhesion to extracellular matrix proteins by upregulating integrin activation
... FLSs were incubated for 1 hour with 10 -7 M adrenome- dullin and washed. Then, 10 5 cells were plated on eight- well LabTek uncoated coverslips (Nunc A/S) for 2 hours at 37°C. After incubation, nonadherent cells were ...
11
N-Succinyl-chitosan nanoparticles coupled with low-density lipoprotein for targeted osthole-loaded delivery to low-density lipoprotein receptor-rich tumors
... CS (deacetylation degree, 93.1%; molecular weight, 8–10 kDa) was provided by Xingcheng Biochemical Co, Ltd (Nantong, People’s Republic of China). Succinic anhydride was purchased from Aladdin Reagents (Shanghai, People’s ...
14
Isolation and characterization of a glycosaminoglycan with anticancer activity
... Annexin V, an anticoagulant with other biological effects, is a member of a family of proteins which exhibits Ca 2+ -dependent binding to negatively charged phospholipids (Allen et al 1997). Annexin V binds strongly and ...
324
The cathepsin S cysteine proteinase of the burrowing nematode Radopholus similis is essential for the reproduction and invasion
... with FITC and octopamine The effects of FITC and octopamine on nematode activity and the effect of octopamine on dsRNA solution uptake by ...using FITC as a visual ...with FITC or octopamine ...
15
The SH3 and cysteine-rich domain 3 (Stac3) gene is important to growth, fiber composition, and calcium release from the sarcoplasmic reticulum in postnatal skeletal muscle
... 9 Muscle strength analysis of Stac3 fl/fl , Stac3 +/fl Tg Cre , and Stac3 fl/fl Tg Cre mice before and after tamoxifen injection. Muscle strength was assessed by grip strength and grip t[r] ...
17
GATA4 and GATA6 regulate pancreatic endoderm identity through inhibition of hedgehog signaling
... Gata6 fl/fl ; Pdx1:Cre ( pDKO) embryos is accompanied by the transition of ventral and dorsal pancreatic fates into intestinal or stomach lineages, ...
7
Effects of CALL on Self-directed FL Vocabulary Learning
... This paper focuses on whether computer technologies could help to create a better learning environment for FL vocabulary learners in China, activating their utilization of various voca[r] ...
26
Sendai Virus Envelopes Can Mediate Epstein-Barr Virus Binding to and Penetration into Epstein-Barr Virus Receptor-Negative Cells
... Adsorption of FITC-EBV and unlabeled EBV with SV-treated cells EBNA induction" in BJA-B cells by residual EBV' % EBNA positive Residual FITC-EBVa binding EBV adsorption with: to Raji cel[r] ...
8
NMDA receptors mediate synaptic depression, but not spine loss in the dentate gyrus of adult amyloid Beta (Aβ) overexpressing mice
... CT100(I716F) overexpression and NMDAR subunit knockout did not affect total length and arborisation of granule cell dendrites except for subtle changes in den- dritic complexity in CT100(I716F) overexpressing cells ...
20
Innate Signals in the Macrophage Determine the Severity of Toxoplasma Gondii infection
... Socs3 fl/fl mice allowed these mice to control the acute challenge with ...Socs3 fl/fl mice treated with a lethal dose of LPS had limited TNF-α production and survived the sepsis caused by ...
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