fluorescence correlation spectroscopy
Direct Vpr Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging
... Hugues de Rocquigny* - [email protected] * Corresponding author Abstract Background: The human immunodeficiency virus type 1 (HIV-1) encodes several regulatory proteins, notably Vpr which influences the ...
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Precise quantification of cellular uptake of cell-penetrating peptides using fluorescence-activated cell sorting and fluorescence correlation spectroscopy
... 2.3.3. Fluorescence correlation spectroscopy (FCS) FCS was used to measure the exact amount of CPPs taken up by the cells in the lysate. FCS is a quantitative technique with single molecule ...
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Characterization of diblock copolymer order-order transitions in semidilute aqueous solution using fluorescence correlation spectroscopy
... Preparation of binary mixtures of rhodamine-labelled and unlabelled HOOC-PGMA 43 - P(HPMA 119 -co-GlyMA 1 ) worm gels The 10% w/w rhodamine B piperazine-labelled HOOC-PGMA 43 —block-P(HPMA 119 -co- GlyMA 1 ) worm gel ...
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Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins.
... The cells were finally suspended in 100 μL of DPBS and transferred to microcentrifuge tubes prior to obtaining flow cytometry measurements. Fluorescence Correlation Spectroscopy. FCS measure- ments ...
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Advancements in methodologies and theories regarding model membrane environments by total internal reflection with fluorescence correlation spectroscopy
... 2.1 Abstract The diffusion coefficients of nine fluorescently labeled antibodies, antibody fragments and antibody complexes have been measured in solution very close to supported planar membranes by using total internal ...
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The use of fluorescence correlation spectroscopy to characterize the molecular mobility of fluorescently labelled G protein coupled receptors
... complexes. Fluorescence correlation spectroscopy (FCS) is a technique with single cell sensitivity that sheds light on the molecular dynamics of fluorescently labelled receptors, ligands or signalling ...
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Perspective: Chain dynamics of unfolded and intrinsically disordered proteins from nanosecond fluorescence correlation spectroscopy combined with single-molecule FRET
... with fluorescence correlation spectroscopy has opened a new window for probing global chain dynamics, but a comprehen- sive picture will require additional information from comple- mentary ...
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Direct Observation of T4 Lysozyme Hinge-Bending Motion by Fluorescence Correlation Spectroscopy
... used fluorescence fluctuation spectroscopy to directly detect T4L equilibrium conformational fluctuations in ...conformations. Correlation analysis of Tetramethylrhodamine intensity fluctuations ...
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Observation of Single-Molecule Rotational Diffusion at Microsecond Timescale by Polarized Fluorescence Correlation Spectroscopy
... fluctuation spectroscopy are triplet-state ‘blinking’ of fluorescent dyes, 302, 303 and quantum yield fluctuations of semiconductor nanocrystals, 304–308 both of which are photosensitive processes strongly ...
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Fluorescence correlation spectroscopy on electron transfer reactions : probing inter- and intramolecular redox processes
... oxidized, fluorescence from the label is reduced by FRET to the Cu site, whereas for reduced azurin the fluorescence of the label is not affected by ...
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Characterization of diblock copolymer order-order transitions in semidilute aqueous solution using fluorescence correlation spectroscopy
... Each measurement was made for 6 s and repeated 150 times. Measurements with average count rates less than 1 kHz were discarded. To confi rm conjugation of the RhB dye label to the PGMA- PHPMA copolymer chains, preliminary ...
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March 2010 No. 36. Fluorescence Correlation Spectroscopy : The Femtoliter Test Tube System Calibration and In Vitro Applications
... FLCS Fluorescence lifetime correlation spectroscopy (FLCS) uses time-resolved fl uorescence measu- rements in more than one ...by correlation analysis (FCS) as well as in terms of fl uorescence ...
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Fluorescence correlation spectroscopy, combined with bimolecular fluorescence complementation, reveals the effects of β arrestin complexes and endocytic targeting on the membrane mobility of neuropeptide Y receptors
... 3.3. Generation of novel sfGFP BiFC partners for detection of receptor – arrestin association Complementary FCS and FRAP approaches both demonstrated a clear correlation between agonist effects on Y1 and Y2 ...
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The Measurement of Sensitivity in Fluorescence Spectroscopy
... large fluorescence signal In certain experiments, the researcher is looking in a time-based mode for a small change in the level of a large ...on fluorescence, and in ratio fluorescence using probes ...
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Simulation of Fluorescence Spectroscopy Experiments
... 66 6. FLUORESCENCE ANISOTROPY OF A FREE DYE ratios as in the experiments. All systems were energy minimized to obtain the starting configuration for the simulations. The solvent and the dye were jointly coupled to ...
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Time-Integrated Fluorescence Cumulant Analysis in Fluorescence Fluctuation Spectroscopy
... This requirement is problematic since FFS data are cor- related. For all experimental conditions we tested, we found that the moments-of-moments variance of Var M ½ˆk ½r is sufficiently accurate for r . 2. For r ¼ 1, ...
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Tunable supercontinuum source for fluorescence spectroscopy
... cyclohexane fluorescence decay ...Fluorescein fluorescence excitation spectra in ...fluorescein fluorescence decays with varying excitation wavelength (430-515 nm), emission=520 ...
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Cumulant Analysis in Fluorescence Fluctuation Spectroscopy
... in Fluorescence Fluctuation Spectroscopy Joachim ...introduced. Fluorescence cumulant analysis (FCA) exploits the factorial cumulants of the photon counts and resolves heterogeneous samples based on ...
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Fluorescence spectroscopy for wastewater monitoring: A review
... C fluorescence compared to natural waters 837 Peak T reduced after biological treatment and peak C after chlorination and RO 838 Fluorometers measuring peaks T and C may be used for online wastewater monitoring ...
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Characterization of Vegetable Oils by Fluorescence Spectroscopy
... of correlation, the variable AV_l2 and AV_l3 show strong correlation and are best represented than the ...third fluorescence peaks corre- sponding to oxidation products to discriminate between ...
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