fluorescence recovery
Using Fluorescence Recovery After Photobleaching (FRAP) to study dynamics of the Structural Maintenance of Chromosome (SMC) complex in vivo
... Above: Representative time-lapse of a cell with MukB-YFP foci during a FRAP experiment is shown. The region of interest (ROI) that is pulse-bleached is highlighted with a circle, pulse- bleaching is indicated with * and ...
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Glucocorticoid Receptor Mutants Demonstrate Increased Motility Inside the Nucleus of Living Cells: Time of Fluorescence Recovery After Photobleaching (FRAP) Is an Integrated Measure of Receptor Function
... Using fluorescence recovery after photobleaching (FRAP) analysis, all GR pathologic mutant receptors examined, as well as 2 synthetic GR mutants lacking the activation function (AF)-1 or the lig- ...
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Controllable Autocatalytic Cleavage-Mediated Fluorescence Recovery for Homogeneous Sensing of Alkyladenine DNA Glycosylase from Human Cancer Cells
... FAM-BHQ1 fluorescence resonance energy transfer (FRET) pair, leading to the amplified fluorescence ...of fluorescence strategy, the proposed method can provide a facile and robust biosensing platform ...
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Assessing cellular efficacy of bromodomain inhibitors using fluorescence recovery after photobleaching
... no recovery after photobleaching (data not shown), indicating that BRD1 is almost entirely immobilised in these speckles within the time frame of the FRAP ...FRAP recovery time of wild-type cells, but not ...
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Investigating the effect of proteoglycan 4 on hyaluronan solution properties using confocal fluorescence recovery after photobleaching
... The objectives of this study were to 1 use confocal FRAP to examine the specific and dose-dependent effect of PRG4 on HA solution networks by analyzing the diffusion of a fluorescein iso[r] ...
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Frequent exchange of the DNA polymerase during bacterial chromosome replication
... focus fluorescence recovered in a few seconds for Pol III and clamp loader components (Figure ...1B). Fluorescence recovery is not explained by the photophysical properties of YPet, like ...
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Diffusion of protease inhibitors in the muscle cell
... and fluorescence recovery after photobleaching (FRAP) by laser scanning confocal microscopy (LSCM) were used to measure the translational diffusion of fluorescein isothiocyanate (FITC)- labeled protease ...
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Protein diffusion in Escherichia coli
... This dissertation describes the creation of a system to provide insight about the affects of protein expression on intracellular diffusion. Fluorescence recovery after photobleaching (FRAP) is used to ...
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Using bacterial inclusion bodies to screen for amyloid aggregation inhibitors
... the fluorescence of native untagged GFP (Figure ...GFP fluorescence recovery, its effect was clearly lower than the one exerted on the refolding of Aβ42wt-GFP IBs (Figure 2B), indicating that in both ...
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Analysis of Borna Disease Virus Trafficking in Live Infected Cells by Using a Virus Encoding a Tetracysteine-Tagged P Protein
... used fluorescence recovery after photobleaching (FRAP) to photobleach a circular defined region of the nucleus or the cytoplasm, and the time taken to refill the photobleached area was ...incomplete ...
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Aptavalve-gated Mesoporous Carbon Nanospheres image Cellular Mucin and provide On-demand Targeted Drug Delivery
... and fluorescence recovery. Interestingly, the recovered fluorescence intensity was linear to the MUC1 concentration with a linear range of ...for fluorescence measurement, the detection limit ...
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Polycomb group protein complexes exchange rapidly in living Drosophila
... (fluorescence recovery after photobleaching, FRAP) on GFP fusion proteins of Polycomb (Pc) and Polyhomeotic (Ph), two essential members of the PCC in whole living Drosophila embryos and larval tissues to ...
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Distribution and Dynamics of Transcription-Associated Proteins during Parvovirus Infection
... Our previous studies indicated that in CPV infection, part of the enhanced cyan fluorescent protein (ECFP)-PML foci were sit- uated in close proximity to or colocalized with the CPV NS1 pro- tein in infected cells. Here, ...
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Identification of biomolecule mass transport and binding rate parameters in living cells by inverse modeling
... Transport of biomolecules in small systems such as living cells is a function of diffusion, reactions, catalytic activi- ties, and advection. Innovative experimental protocols and mathematical modeling of the dynamics of ...
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Studying lateral mobility of surface molecules on the plasma membrane using biophysical approaches
... Fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS) and Single particle tracking (SPT) are common biophysical methods to measure lateral mobi[r] ...
102
5828.pdf
... as fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) mask the underlying mechanism of the anomalous ...
254
Photobleaching of red fluorescence in oral biofilms
... Local macromolecule diffusion coefficients in structurally nonuniform bacterial biofilms using fluorescence recovery after photobleaching FRAP.. Induction of lethal photosensitization in[r] ...
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Negative regulation of EB1 turnover at microtubule plus ends by interaction with microtubule-associated protein ATIP3
... By fluorescence recovery of EB1- GFP after photobleaching, we show that ATIP3 silencing accelerates EB1 turnover at microtubule ends with no modification of EB1 diffusion in the ...
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Novel Method for Evaluating In Vitro Activity of Anidulafungin in Combination with Amphotericin B or Azoles
... of fluorescence intensity of ...the fluorescence of untreated cells stained with FUN-1, line c is the fluorescence of cells treated with 70% ethanol and stained with FUN-1 (positive control), and ...
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Photosynthetic Gas Exchange and Chlorophyll Fluorescence as Drought Tolerance Indicators in Citrus Rootstocks under Water Stress and Recovery
... of recovery in stomatal conductance were major obstructions in photosynthesis recovery, ...better recovery ability and Mexican lime plants had profound ability to recover from imposed negative ...
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