fluorometric assay
A Rapid, Automated Enzymatic Fluorometric Assay for Determination of d Arabinitol in Serum
... the fluorometric assay described here, the coupling reagent and dye reagent are eliminated, saving both time and cost in reagent preparation and reducing potential sources of ...one assay, whereas ...
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Fluorometric Assay for Testing Rifampin Susceptibility of Mycobacterium tuberculosis Complex
... In this article, we report the development of a novel PCR-based fluorometric assay for testing of susceptibility to rifampin, called FAST-Rif. This method allowed for the rapid detection of 15 different ...
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A fluorometric assay for trehalose in the picomole range
... is essentially linear with respect to the concentration of trehalose. To calibrate the assay, it is necessary to gener- ate an internal standard curve for each sample. This is done by addition of known amounts of ...
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Comparison of NADH and NADPH oxidase activities in granules isolated from human polymorphonuclear leukocytes with a fluorometric assay
... leukocytes. In the presence of strong base the oxidized forms of the pyridine nucleotides form a highly fluorescent product. The small amounts of NAD(P) formed by the oxidase reactions can be determined with great ...
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Rapid Fluorometric Assay of Bilirubin and Bilirubin Binding Capacity in Blood of Jaundiced Neonates: Comparisons with Other Methods
... The values for total binding capacity by he- matofluorometry, taken as the sum of the readouts for bound biirubin and reserve binding capacity, and by gel filtration, taken as the lowest[r] ...
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Simultaneous detection of Human Immunodeficiency Virus 1 and Hepatitis B virus infections using a dual-label time-resolved fluorometric assay
... immunofluorometric assay was developed exploiting the unique emission wavelengths of the intrinsically fluorescent terbium (Tb 3+ ) and europium (Eu 3+ ) tracers for the simultaneous detection of human ...
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Rapid fluorometric quantification of bacterial cells using Redsafe nucleic acid stain
... Redsafe fluorometric assay is 2-log unit less than that needed for tradition MTT as- ...fluorescent assay can be used to count all type of bacterial cells but MTT assay cannot be applied on ...
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New Commercially Available IgG Kits and Time-Resolved Fluorometric IgE Assay for Diagnosis of Allergic Bronchopulmonary Aspergillosis in Patients with Cystic Fibrosis
... (ii) Time-resolved fluorometric assay: DELFIA. Preparation of PPE of A. fumigatus (IHEM 22670) was obtained by culture for 7 days on a DG18 solid medium, and the conidia were then gently scraped off the ...
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Analysis for petroleum products in marine environments
... For example, techniques such as high-performance liquid chromatography (HPLC), in conjunction with on-line fluorometric assay techniques and mass spectrometry, make possible the analy[r] ...
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Comparison of Beta Glucuronidase Expression and Anatomical Localization in Bombarded Immature Embryos of Banana Cultivar Mas via Biolistic Transformation
... Fluorometric assay. The fluorometric assay for specific GUS enzyme activity was quantified by measuring the hydrolysis rate of the fluorogenic substrate 4- methylumbelliferyl â-D-glucuronide ...
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Alternative Methods for Analysis of Cyanobacterial Populations in Drinking Water Supplies: Fluorometric and Toxicological Applications Using Phycocyanin
... the PC levels in all size fractions were highest and there was a visible surface scum. The extracellular/total WLW ratios for these three samples ranged from 22% - 28%, with an average of 24%. Bastien, et al . [36] ...
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Complex formation of platelet thrombospondin with plasminogen Modulation of activation by tissue activator
... immunoelectrophoresis systems, complex formation was inhibited by 10 mM epsilon-amino- n-caproic acid, implying that there is a role for the lysine binding sites of Plg in mediating the interaction. TSP also formed a ...
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Sensitive fluorometric method for the determination of rutin in combined tablet dosage form
... Considering the effects of foreign substances on the fluorescence intensity of the system, the standard addition method was used for the determination of rutin in compound rutin table[r] ...
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Newborn Screening for Phenylketonuria: Predictive Validity as a Function of Age
... Blood Phenylalanine Concentrations Measured by Fluorometric or Column Chromatographic Methods in Patients with Phenylketonuria (PKU) and Control Subjects During First 2 Weeks of Life-Log[r] ...
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Novel method using o hydroxyphenylthiourea for cadmium sensing in water samples †
... Into 10mL volumetric flasks an aliquot of cadmium(II) solutions (800ng/mL-10ng/mL) were transferred. To each flask, 1mL of 1%bathophenanthroline, and 1mL of 1% sodium dodecylsulphate were added and pH was adjusted to 9.0 ...
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Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli
... The on-line visualization of efflux activity by the EtBr loaded cells requires that accumulation of EtBr has previ- ously taken place. Therefore, before performing the efflux assays, bacteria are exposed to conditions ...
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Early Detection of Pompe Disease by Newborn Screening Is Feasible: Results From the Taiwan Screening Program
... the assay wells were cal- culated by linear regression from the standard curve; GAA activity is presented as mol/L whole blood (Wb) per ...the assay protocols were noted: in November 2005, the ...
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T DNA promoter tagging in Nicotiana tabacum : a thesis presented in fulfilment of the requirements for the degree of Master of Philosophy in Genetics at Massey University, Palmerston North, New Zealand
... Comparison of the fluorometric 3 7% positive, 87 plants tested and histochemical 22% positive, 147 plants tested screens for GUS activity in root and shoot material was discussed and it [r] ...
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Silica nanoparticles inhibit the cation channel TRPV4 in airway epithelial cells
... Results: Using fluorometric measurements of intracellular Ca 2+ concentration ([Ca 2+ ]i) we found that SiNPs inhibit activation of TRPV4 by the synthetic agonist GSK1016790A in cultured[r] ...
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A Microfluidic Platform for Single Cell Fluorometric Granzyme B Profiling
... The use of the microfluidic platform in the single cell GrB activity assay has shown variation in the GrB activity between the healthy donor’s PBMC and that of the patient’s. Increase in GrB expression, as well as ...
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