Gene copy number analysis by Taqman method
TaqMan Copy Number Assays. Protocol
... The TaqMan ® Genotyping Master Mix, containing AmpliTaq Gold ® DNA Polymerase, UP (Ultra Pure) and dNTPs required for the PCR ...data analysis of copy number quantitation ...
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Gene Copy-Number Alterations: A Cost-Benefit Analysis
... of gene copy-number changes in developing ...the gene products amplified/deleted in partic- ular ...a gene encoding the GABA A receptor subunit, has been associated with autism spectrum ...
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Analysis of gene copy number changes in tumor phylogenetics
... (DP) method of [36] can be adapted to find the internal labels, but modifications are needed to account for the rectilinear metric and its implications on the total tree ...
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Phylogenetic Reconstruction Analysis on Gene Order and Copy Number Variation
... cancer copy number ...cells. Copy number profiles provide a tool to better understand how the genomic structure alteration occurs from the ...phylogenetic analysis of copy ...
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SMASH, a fragmentation and sequencing method for genomic copy number analysis
... The chimeric sequence reads generated by SMASH are pro- cessed using a time-efficient, memory-intensive mapping algo- rithm that performs a conservative partition of the long read into constituent fragments. The fragment ...
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qKAT: a high-throughput qPCR method for KIR gene copy number and haplotype determination
... and copy number variation makes KIR s technically laborious and expensive to ...reaction method to determine gene copy number for each KIR ...precise copy number ...
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A robust penalized method for the analysis of noisy DNA copy number data
... relative copy num- bers of the neighboring markers ...tive copy numbers along the ...the copy number ...small number of markers have some nonzero ...This method is applied to ...
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Characterization of single gene copy number variants in schizophrenia
... this gene set and the relative size, we also performed a larger validation of deletions using a higher throughput method that again confirmed 4 qPCR validated single-gene deletions in cases as well ...
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Importance of rare gene copy number alterations for personalized tumor characterization and survival analysis
... of gene expression levels for cancer cell lines and tumor patients. Gene-specific correlations between predicted and originally measured gene expression levels of individual genes comparing CCTN ...
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Plasmodium copy number variation scan: gene copy numbers evaluation in haploid genomes
... The analysis of CNV identification on three genes known to have different levels of amplification and which are located either in the nuclear, apicoplast or mitochondrial genomes is ...
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Plasmodium copy number variation scan: gene copy numbers evaluation in haploid genomes.
... The analysis of CNV identification on three genes known to have different levels of amplification and which are located either in the nuclear, apicoplast or mitochondrial genomes is ...
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TaqMan Gene Expression Assays. Protocol
... data analysis tool for sample comparison when using the comparative C T (ΔΔC T ) method for calculating relative quantitation of gene ...quantification analysis of gene expression ...
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Gefitinib and EGFR Gene Copy Number Aberrations in Esophageal Cancer.
... mutation analysis may not be representative of the intention-to-treat population from the original ...and analysis were hypothesis driven, performed to diagnostic standard in a reference laboratory with ...
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Low copy number of the FCGR3B gene and rheumatoid arthritis: a case control study and meta analysis
... Copy number, linkage disequilibrium and disease association in the FCGR ...3B gene copy number with rheumatoid arthritis in Caucasian ...
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Integrated analysis of copy number alterations and gene expression: a bivariate assessment of equally directed abnormalities
... 2 METHODS 2.1 Segmentation of CN data CN and GE in general are measured on different platforms, i.e. possibly not at the same locations and based on DNA sections of different lengths. As a consequence of the differences ...
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Expression of the immunoglobulin VH gene 51p1 is proportional to its germline gene copy number
... H gene that is frequently ex- pressed in B cell chronic lymphocytic leukemia and early in B cell ...51p1 gene locus is highly polymorphic, consisting of 13 alleles that can be classified as being either ...
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Analysis of Germline Gene Copy Number Variants of Patients with Sporadic Pancreatic Adenocarcinoma Reveals Specific Variations
... The analysis revealed that the number of abnormal SNP probe hybridization signals, relative to the HapMap set, ranged from 22,284 to 56,664 (median value 33,621) in patients with sporadic pancre- atic ...
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Genome-wide analysis of copy number variations identifies PARK2 as a candidate gene for autism spectrum disorder
... PARK2 gene possibly plays a role in the pathology of devel- opmental anomalies ...expression analysis, we speculate that the copy number gain may lead to a splicing error; hence, a shorter ...
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Chromosome 9p21 gene copy number and prognostic significance of p16 in ESFT
... CDKN2A gene status, p16/p14 ARF mRNA and p16 protein expression was evaluated using the log-rank ...the number of events that may have ...CDKN2A gene status, p16/p14 ARF mRNA expression and clinical ...
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Human amylase gene copy number variation as a determinant of metabolic state
... randomization analysis and prospective data collected after 9 years of follow-up, the authors found a causal negative effect of BMI on plasma amylase activity; when they used a linear regression model to ...
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