Gene Editing
From fiction to science: clinical potentials and regulatory considerations of gene editing
... β-globin gene [42, 43], resulting in faulty synthesis or mutant variants of the β-globin chain of ...γ-globin gene expres- sion ...suitable gene editing candidate for the treatment of the ...
16
Use of gene-editing technology to introduce targeted modifications in pigs
... that gene- editing and gene-stacking technology can efficiently gener- ate pigs carrying multiple knockout genes to serve as a model for xenotransplantation ...The gene-editing technol- ...
10
Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species
... for gene editing in ...plasmid-based gene editing system are that any gene can be edited in any isolate in a markerless ...for editing in Candida species other than ...GFP ...
14
Vigour of CRISPR/Cas9 Gene Editing in Alzheimer’s Disease
... HTT gene, which ciphers for mutant hunting in (mHTT), a large protein consisting of large polyglutamine repeats in the N-terminal domain of mHTT that most likely features a toxic-gain of function ensuing in ...the ...
5
CRISPR-Cas, a robust gene-editing technology in the era of modern cancer immunotherapy
... of gene-editing tools (ZFN, TALEN, and CRISPR) to knock out, knock down or modify endog- enous TCR is the principal technique [92, ...nuclease-based gene-editing tools by Osborn et ...
17
Gene editing towards hypoimmunogenic gluten proteins in wheat
... epitopes. Gene editing using CRISPR/Cas9 can precisely remove or modify the DNA sequences coding for immunogenic ...of gene editing for improving crops for human consumption where conventional ...
214
Falling giants and the rise of gene editing: ethics, private interests and the public good
... of gene editing, and in particular CRISPR, we consider how commercialisation encourages hype and hope — a sense that only promise and idealism can achieve ...
10
The emerging role of viral vectors as vehicles for DMD gene editing
... DMD editing strategies to generate genetically corrected human cells with myoregenerative capacity for autologous transplantation can also be envisaged (Table ...particles, gene-editing tools, and ...
10
New Gene Therapy Strategies for the Deletion of Exon 44 of Dystrophin Gene Based on Gene Editing by TALENs
... modification, gene disruption, gene addition, and gene correction, can be applied in gene therapy of human diseases such as hemophilia, thalassemia, DMD, neuronal deafness, Stargardt’s ...
6
Heritable human gene editing in global context : national and international policy challenges
... heritable gene editing is prohibited collaborate with IVF clinics in more leniently regulated countries to achieve first-in- human applications in a legal gray area, transnational germline gene ...
14
Gene editing restores dystrophin expression in a canine model of Duchenne muscular dystrophy
... vivo gene editing is a further potential safety ...off-target gene editing above background levels in treated animals, it will be important to further assess possible off-target mutagenesis in ...
6
Efficient gene editing in Neurospora crassa with CRISPR technology
... cient gene replacements utilizing CRISPR/Cas9 tech- nology in a model filamentous fungus, ...established gene editing protocols in Table 2 Primers used in this ...
7
CRISPR/Cas9 mediated gene editing ameliorates neurotoxicity in mouse model of Huntington’s disease
... CRISPR/Cas9-mediated gene editing could be used to efficiently and permanently eliminate polyglutamine expansion–mediated neuronal toxicity in the adult ...
7
Two Distinct Approaches for CRISPR-Cas9-Mediated Gene Editing in Cryptococcus neoformans and Related Species
... Over the past several years, advanced methodology that employs meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic ...
9
Manipulating plant RNA silencing pathways to improve the gene editing efficiency of CRISPR/Cas9 systems
... efficient gene editing ...(TT4) gene was selected as the CRISPR target (Fig. 1b). Mutations in this gene stop the production of brown pig- ment in Arabidopsis seeds providing an easily ...
15
Functional genetics for all: engineered nucleases, CRISPR and the gene editing revolution
... Double-strand breaks can elicit two types of molecular repair mechanism at the site of damage: non-homologous end joining (NHEJ) in which the broken ends are re- ligated to each other, or homology-directed repair (HDR) ...
13
Crispr/Cas9 System - A Revolution in Gene Editing
... of gene drives: the development of disease-resistant mosquitoes that could knock out Malaria and ...Zika. Gene editing can now change an entire species forever. CRISPR gene drives allow ...
11
Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
... In addition, it is difficult to express some high molec- ular weight proteins in C. glutamicum; therefore, we codon-optimized the Cas9 gene and an SD sequence was added in front of the ATG initiation codon. We ...
13
Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli
... create gene knock- outs and knock-ins in prokaryotes (for a recent review see Choi and Lee ...genome editing strategy, including the procedure based on the λ Red/synthetic oligonucleotide-mediated homolo- ...
18
Gene Editing by Dave Barnhart
... Food is another area of daily life where CRISPR could have potential uses. Traditional GMO (genetically modified organism) foods have often been controver- sial. GMOs take genes from one organism and insert them into ...
6