Gene expression signals for GAPDH (120 bp) and collagen
The impact of bisphosphonates on the osteoblast proliferation and Collagen gene expression in vitro
... As a quantitative RT-PCR we used the SYBR Green Real Time PCR (oneStep RT-PCR, Bio-Rad, Hercules, CA/USA). This method enables reverse transcription using the individual primers immediately before PCR amplification and ...
6
Role of protein kinase C δ in the regulation of collagen gene expression in scleroderma fibroblasts
... of collagen gene expression in SSc by assessing the levels of PKC- δ in normal and SSc cells using high-resolution fluorescence immu- nomicroscopy and Western analyses of cell ...of expression ...
10
Transcriptional regulation of the collagen alpha1(1) gene by hypoxia
... Nuclear proteins or cytoplasmic contaminants? There are a number of proteins identified that are not traditionally regarded as “nuclear proteins”. These include GAPDH, protein kinase C and collagen a chain ...
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GAPDH expression as a measurement of transfection efficiency for p16INK4a gene silencing (siRNA) in senescent human diploid fibroblasts
... using GAPDH siRNA as a positive ...of GAPDH mRNA expression indicating the transfection method was optimised for the HDFs aging model used in the ...
8
Kinetics of gene expression and bone remodelling in the clinical phase of collagen induced arthritis
... Distinctive expression profiles of genes involved in osteoblast and osteoclast functions The differentially expressed genes, related to bone, from Table S10 in Additional file 10, were divided into profiles and ...
18
Gene expression profile and synovial microcirculation at early stages of collagen induced arthritis
... reduce expression of adhesion molecules and to decrease cellularity of rheumatoid synovial tissue [29,30], supporting the hypothesis that the anti-inflammatory effect is due to a downregulation of ...
9
Collagen V-induced nasal tolerance downregulates pulmonary collagen mRNA gene and TGF-beta expression in experimental systemic sclerosis
... The collagen synthesis normalization in the experimen- tal animals after the tolerance induction with collagen V, discloses, for the first time, the possibility of avoid fibrosis progression in an SSc ...
10
Suppression of Type I Collagen Gene Expression by Prostaglandins in Fibroblasts Is Mediated at the Transcriptional Level
... Because the cAMP-responsive DNA bind- ing protein CREB may be phosphorylated by either protein kinase A or Ca 2 -dependent ki- nases (54,55), it could be involved in signaling by any of the different EP and FP receptor ...
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Gene expression profiling and functional analysis of angiogenic markers in murine collagen induced arthritis
... arthritis. Gene expression profiling of murine CIA has been conducted before ...angiogenesis-relevant gene expression in CIA, the present study applied quantitative reverse transcription ...
18
Plant-parasitic nematodes respond to root exudate signals with host-specific gene expression patterns
... the expression of selected cell-wall degrading enzyme genes relative to the abundance of substrate in root exudates, thereby tailoring gene expression for root entry of the immediate ...specific ...
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Early Polyadenylation Signals of Human Papillomavirus Type 31 Negatively Regulate Capsid Gene Expression
... viral gene expression ...degenerative signals, AGUAAA ...of expression of viral capsid ...viral expression and copy ...of expression is more beneficial for the long-term ...
11
Gcn4p, a Master Regulator of Gene Expression, Is Controlled at Multiple Levels by Diverse Signals of Starvation and Stress
... It is known that refeeding glucose to glucose-starved cells triggers a transient increase in cAMP synthesis that is depen- dent on the RAS proteins and the G protein subunit Gpa2p (13). Interestingly, this regimen ...
11
Interleukin 10 modulates type I collagen and matrix metalloprotease gene expression in cultured human skin fibroblasts
... promoter/chloramphenicol acetyl transferase reporter gene constructs showed downregulation by IL-10, suggesting inhibition at the transcriptional level. When compared with control cultures, incubation with IL-10 ...
5
Type of Aerobic Training Effect on Cardiac Muscles MIR29A and Collagen I Gene Expression in Diabetic Male Rats
... regulated collagen IV and reduction of MIR29A caused by high glucose level in serum may lead to increase of collagen deposition in proximal tubule of kidney (12) MIR29A has a key role in physiologic cardiac ...
8
Interactions between inflammatory signals and the progesterone receptor in regulating gene expression in pregnant human uterine myocytes
... compare gene expression between cells in the presence or absence of progesterone as there is no withdrawal of progesterone in associa- tion with human labour and therefore, in vivo, there is always PR ...
18
Effects of Altering the Transcription Termination Signals of Respiratory Syncytial Virus on Viral Gene Expression and Growth In Vitro and In Vivo
... viral gene expression and that replica- tion in the respiratory tract of mice might be more ...the gene junctions (2, 3, 11, 14, 18, 25, ...the gene in question and its immediate downstream ...
8
d alpha tocopherol inhibits collagen alpha 1(I) gene expression in cultured human fibroblasts Modulation of constitutive collagen gene expression by lipid peroxidation
... stimulates collagen gene transcription in cultured fibroblasts, and this effect is mediated through the induction of lipid peroxidation by ascorbic ...of collagen production, but the mechanisms of ...
7
Gene expression profile of collagen types, osteopontin in the tympanic membrane of patients with tympanosclerosis
... the gene expression profile of COL1A1, COL2A1, COL3A1, COL4A1 and SPP1 in the tympanic membrane of patients with tympano- ...of gene profile expression of the collagen types and ...
6
Mithramycin selectively inhibits collagen alpha 1(I) gene expression in human fibroblast
... Mithramycin interferes with Sp1 but not with NF-I binding to these sites. At a concentration of 100 nM, mithramycin efficiently reduces basal and TGF-beta-stimulated alpha 1(I) gene expression in human ...
7
Effects of Glycine in Collagen Expression and Factors
... group supplemented with glycine higher amounts of collagen expression and predominance type 25.. of collagen I.[r] ...
12