gene targeting
Conditional gene targeting
... the gene target- ing field [14, ...a gene targeting experiment the selection marker gene on the targeting vector (subse- quently inserted into the target locus by homologous recombi- ...
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Gene targeting in plants: 25 years later
... ABSTRACT Only five years after the initiation of transgenic research in plants, gene targeting (GT) was achieved for the first time in tobacco. Unfortunately, the frequency of targeted integration via ...
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Modification of the apolipoprotein B gene in HepG2 cells by gene targeting
... whether gene-targeting techniques can be used to inactivate one of the apo B alleles in HepG2 cells by homologous recombination using a transfected gene-targeting ...B gene, in which ...
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Creation of non-human primate neurogenetic disease models by gene targeting and nuclear transfer
... human gene of interest, as is the case for Kallmann's syndrome, or because mutant mice do not exhibit the same phenotype observed in humans, as is the the case for Lesch- Nyhan's disease and ...However, ...
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Gene targeting with a replication-defective adenovirus vector.
... the gene-targeting technique has been hampered by its low level of ...neo gene inserted in the adenovirus vector and a target mutant neo gene on a nuclear papillomavirus ...allowed gene ...
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Gene targeting as a tool to investigate granulocyte function
... al gene targeting an e s s e n t i a l part o f the c h o s e n gene is fl an ke d by l o x P sites in the same ...al targeting requires two things: a f l o x e d all el e o f the c h o s e n ...
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An Enhanced Gene Targeting Toolkit for Drosophila: Golic+
... Ends-out gene targeting allows seamless replacement of endogenous genes with engineered DNA fragments by homologous recombination, thus creating designer “genes” in the endogenous ...Conventional ...
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The Molecular Basis of Multiple Vector Insertion by Gene Targeting in Mammalian Cells
... products they produce are depicted. Each gene-targeting reaction has the potential to generate a different marker pattern in each recombinant. For this reason, the identity of the diagnostic restriction ...
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Nanos3 Gene Targeting in Medaka ES Cells
... Gene targeting (GT) by homologous recombination offers the best precision for genome editing in ...conserved gene and encodes a zinc-finger RNA binding protein essential for germ stem cell ...
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Triplex Forming Oligonucleotides – Tool for Gene Targeting
... control gene expression has long fascinated researchers because of the potential to rapidly generate potent and specific ...regulate gene expres- sion or to mediate site-specific genome ...and gene ...
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Reproducible gene targeting in recalcitrant Escherichia coli isolates
... the gene disruption method are occasionally required ...Red-mediated gene targeting method [4], occurred at a low and variable frequency and was frequently ...
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Efficient and Heritable Gene Targeting in Tilapia by CRISPR/Cas9
... Reverse genetics approaches have been important in dem- onstrating gene functions, genetic engineering, and un- derstanding complex biological processes. In the present study, we successfully established the ...
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Efficient Gene Targeting in Drosophila With Zinc-Finger Nucleases
... enhanced gene targeting should be broadly useful (P orteus and C arroll ...IL-2Rg gene in cultured cells (U rnov et ...in targeting this gene of therapeutic ...
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TALEN Mediated Gene Targeting for CF Gene Therapy
... targeted gene integration studies as it leads to abundant transgene expression with no adverse consequences ...large gene constructs, such as the CFTR expression cassette [34], can be integrated into this ...
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Gene correction of HBB mutations in CD34+ hematopoietic stem cells using Cas9 mRNA and ssODN donors
... To target the HBB IVS1–110 locus, we designed a sgRNA and several ssODNs (Fig. 1b; Additional file 1: Figure S1) with varying lengths of homology arms, symmetrical dif- ference, and chemical modifications and ...
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Targeted Hsp70 expression combined with CIK-activated immune reconstruction synergistically exerts antitumor efficacy in patient-derived hepatocellular carcinoma xenograft mouse models
... candidate gene in anti-tumor ...targeted gene alone is inadequate to completely stop the growth of ...the gene targeting therapeutic strategy for HCC to enhance the efficacy and safety of the ...
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Gene Deletions by Ends-In Targeting in Drosophila melanogaster
... a gene targeting technique in Drosophila, different methods have been developed to modify the Drosophila ...of gene targeting in flies used an ends-in method, which generates a duplication of ...
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A novel Bifidobacterium infantis-mediated TK/GCV suicide gene therapy system exhibits antitumor activity in a rat model of bladder cancer
... suicide gene targeting system was delivered into tumor-bearing rats, we found that the system can significantly inhibit rat bladder tumor growth, induce apoptosis in tumor cells, and increase Cas- pase 3 ...
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Identification of Mycobacterium Species by PCR Restriction Fragment Length Polymorphism Analyses Using Fluorescence Capillary Electrophoresis
... Polymorphic gene regions were amplified with fluorescently labeled oligonucleotide ...HSP gene (13) was amplified with previously described primers TB11 and TB12 (16) labeled with ...
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A Smart Targeting System for Online Advertising
... smart targeting system for online ...the targeting system to automatically deliver the personalized ...of targeting doesn’t require any sensitive data input by users, so their privacy is ...
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