gene transfection
Effect of gene transfection timing on TGF-β1 expression in rabbit mandibular distraction gap.
... that gene transfection performed during the distraction period lead to higher expression of TGF-β1 than in the other two periods, and therefore, the distraction period is optimal for gene ...
11
Synthesis of cationic porphyrins as potential gene transfection carriers
... to gene transfection efficiency, porphyrins with different number and distribution of hydrophilic and hydrophobic ligands are developed as an alternative to viral-mediated ...as gene marker in the ...
6
Multifunctional Nucleus-targeting Nanoparticles with Ultra-high Gene Transfection Efficiency for In Vivo Gene Therapy
... nanoparticle-based gene delivery system which is capable of targeting and eradicating ...high gene transfection efficiency (>95%) even in culture medium containing 30% serum, which significantly ...
17
Type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes
... influencing transfection efficiency, such as carrier/DNA weight ratio, type of surfactants (CHAPS, NaT and NaO), particle size, zeta potential, morphology, and serum were ...vitro gene transfection ...
9
Calcium-activated gene transfection from DNA/poly(amic acid-co-imide) complexes
... more efficiently released from PA-I polyplexes. These results were consistent with fluorescence expression assays. These experiments also showed that the cationic part of polyimide promoted the dissociation of ...
11
Synthesis and bioassay studies of cationic porphyrins for gene transfection delivery
... in gene therapy that may present higher gene transfection efficiency compared to their non- viral ...of gene delivery ...of transfection efficiency, biocompatibility [28], toxicity ...
37
Use of inert gas jets to measure the forces required for mechanical gene transfection
... of transfection show considerable promise, the effi- ciency of transfection is low compared to viral transfection ...non-viral gene transfection ...high transfection efficiency ...
12
Gene-carried hepatoma targeting complex induced high gene transfection efficiency with low toxicity and significant antitumor activity
... the gene transfection effi- ciency of the free peptide pretreated group was lower than the untreated group (Figure ...the transfection effi- ciency didn’t decrease to zero, suggesting that the ...
12
Gene transfection in primary stem-like cells of giant cell tumor of bone
... viral gene transfection, the GCT cells are resistant to most methods of transient gene ...successful gene transfer in these valuable patient-derived ...
6
Comparison of gene transfection and cytotoxicity mechanisms of linear poly(amidoamine) and branched poly(ethyleneimine) polyplexes
... A549 cells were seeded at a density of 4 × 10 5 cells per well in 6-well plates and incubated for 24 h before transfection. The cells were then treated with various polyplex formulations for 4 h and with FCCP (10 ...
37
A branched TAT cell-penetrating peptide as a novel delivery carrier for the efficient gene transfection
... usability. However, the PEI based gene delivery sys- tems often induce high cytotoxicity problems. In our study, PEI observed to be cytotoxic as indicated in a gray bar in the Fig. 4. However the PEI/pGFP com- ...
8
<p>IP10-CDR3 Reduces The Viability And Induces The Apoptosis Of Ovarian Cancer Cells By Down-Regulating The Expression Of Bcl-2 And Caspase 3</p>
... exotoxin gene muton of Pseudomonas aeruginosa (PE40) to construct IP10-CDR3scfv and IP10-CDR3- ...After gene transfection, SKOV3 cells were divided into four groups: Control, ...NC ...
10
Peptide Mediated Tumor Targeting by a Degradable Nano Gene Delivery Vector Based on Pluronic Modified Polyethylenimine
... non-viral gene vector, Pluronic-PEI-DR5-TAT, was successfully constructed by cross-linking LMW-PEI with Pluronic and further coupled a multifunctional peptide DR5-TAT to form the copolymer for targeting ...
13
Targeted gene delivery in tumor xenografts by the combination of ultrasound-targeted microbubble destruction and polyethylenimine to inhibit survivin gene expression and induce apoptosis
... Successful gene therapy requires safe and efficient delivery systems ...low transfection efficiency and transient gene expression ...the gene transfection in vitro [1], the results of ...
9
Tyrosine hydroxylase gene transfections to different sites of striatum in the rat model of Parkinson’s disease
... of gene therapy has been intensively studied as a potential method to treat Parkinson’s disease (PD) and other degenerative brain ...of gene delivery or on the physiolo- gical state of target tissues have ...
8
NOVEL LIPOSOMAL GENE DELIVERY REAGENTS: DESIGN, SYNTHESIS, IN VITRO AND IN VIVO TRANSFECTION PROPERTIES
... enhanced transfection efficacies of pH-sensitive cationic lipids, covalent grafting of an electron withdrawing substituent in the basic head-group should lead to compromised ...
14
Loss of PIM1 correlates with progression and prognosis of salivary adenoid cystic carcinoma (SACC)
... onco- gene in various human cancers, its potential role as a therapeutic target has not been explored in ...PIM1 gene was inhib- ited in ACC-M cells by constructing short hairpin RNA (shRNA) against PIM1 to ...
11
<p>ANO6 promotes cell proliferation and invasion in glioma through regulating the ERK signaling pathway</p>
... in ANO6-KD1 and ANO6-KD2 groups was signi fi cantly decreased (Figure 2E and F; P<0.05), compared with those of NC group. Thus, to determine whether ANO6 directly affects the ERK signaling activation in T98G and U87MG ...
11
Transfection in Agrobacterium tumefaciens
... Transfection frequencies in our study were optimal with a concentration of 22.5 Ag of phenol-extracted LR-4 phage DNA/ml.. Low DNA concentrations 1 and 10 ,ug/ml were not sufficient to p[r] ...
6
Amphiphilic graft copolymer based on poly(styrene-co-maleic anhydride) with low molecular weight polyethylenimine for efficient gene delivery
... vitro transfection efficiency, cellular uptake, and subcellular location were investigated in MCF-7 and MCF-7/ADR cell lines in the presence of ...high transfection efficiency with low ...
12