Genetic analysis – 16S rRNA encoding gene sequencing
A comprehensive evaluation of the sl1p pipeline for 16S rRNA gene sequencing analysis
... for 16S rRNA gene data processing makes it difficult to identify the most accurate ...library 16S rRNA gene sequencing pipeline (sl1p, pronounced “slip”), a 16S ...
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Species Specific Identification of Leptospiraceae by 16S rRNA Gene Sequencing
... Identification and characterization of leptospiral isolates is based upon polyphasic analysis, with both serological and mo- lecular characterization being essential. For public health pur- poses it has become ...
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Sequence Diversity of Neisseria meningitidis 16S rRNA Genes and Use of 16S rRNA Gene Sequencing as a Molecular Subtyping Tool
... strains, 16S typing was the best discriminatory tool for outbreak-associated versus sporadic ...of 16S rRNA genes and genetic relationship among meningococcal strains of different ...diverse ...
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Catheter Related Microbacterium Bacteremia Identified by 16S rRNA Gene Sequencing
... by 16S rRNA gene analysis (13, ...from 16S rRNA sequence data by the neighbor-joining method and bootstrap values calculated from 1,000 ...
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Species Specific Identification of Campylobacters by Partial 16S rRNA Gene Sequencing
... strains. 16S rRNA gene (16S rDNA)-based identification of bacteria offers a possible alternative when phenotypic tests ...of 16S rDNA sequencing for the species-specific ...
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Controlling for Contaminants in Low-Biomass 16S rRNA Gene Sequencing Experiments
... Another consideration for selecting a contaminant removal method is the format of the results, which may limit downstream analysis. The filtering approaches and Decon- tam classify individual ASVs (or operational ...
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Impact of DNA Sequencing and Analysis Methods on 16S rRNA Gene Bacterial Community Analysis of Dairy Products
... DNA sequencing and analysis pipelines contributed significant variations to OTU/ASV distributions and observed bacterial ...in sequencing scale per run by the use of different sequencing chips ...
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Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification.
... diversity analysis using 97% ...Statistical analysis was performed in Graph Pad using the One-way ANOVA for between group ...MiSeq sequencing platform, V1V2 = bacterial 16S rRNA ...
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Quantification of variation and the impact of biomass in targeted 16S rRNA gene sequencing studies
... absolute 16S rRNA gene copy number and mean relative abundance affected the vari- ance in microbiome ...microbiome sequencing data [30, ...
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reaction and 16S-rRNA gene sequences
... 2019. Genetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16S- rRNA gene ...Identification ...
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Comparison of MALDI TOF MS, Housekeeping Gene Sequencing, and 16S rRNA Gene Sequencing for Identification of Aeromonas Clinical Isolates
... rently 24 valid Aeromonas species reported in the literature. 2 Among them Aeromonas hydrophila, A. caviae, and A. vero- nii biovar sobria are described as the most important human pathogens. 1 Accurate identification of ...
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Application of 16S rRNA Gene Sequencing To IdentifyBordetella hinzii as the Causative Agent of Fatal Septicemia
... CFA analysis is not consistently reliable for identification of ...unique 16S rRNA sequence which was identical among all three strains ...the 16S rRNA sequence of B. hinzii, and ...
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Role of Universal 16S rRNA Gene PCR and Sequencing in Diagnosis of Prosthetic Joint Infection
... Identification of bacteria in only 1 periprosthetic sample by se- quencing can confirm PJI, while confirmation by culture requires the isolation of the same microorganisms in 2 to 3 samples. How- ever, culture is more ...
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Molecular genetic monitoring of bacterial communities in manzala lake, egypt, based on 16S rrna gene analysis
... Keywords: 16S rRNA gene, bacteria diversity, Manzala Lake, ...molecular genetic study on the diversity of bacterial communities at Manzala Lake, Egypt, was determined by culture-independent ...
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Unifying the analysis of high-throughput sequencing datasets: characterizing RNA-seq, 16S rRNA gene sequencing and selective growth experiments by compositional data analysis.
... throughput sequencing experiments are those designed to examine RNA-seq experiments [1], and these tools are often advocated for use in other experimental designs that generate tables of ...
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Unifying the analysis of high-throughput sequencing datasets: characterizing RNA-seq, 16S rRNA gene sequencing and selective growth experiments by compositional data analysis
... high-throughput sequencing stud- ies is to identify those genes or features that make a significant difference between two or more ...RNA sequencing (RNA-seq), chromatin immunoprecipitation ...
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Evaluation of nanopore-based sequencing technology for gene marker based analysis of complex microbial communities. Method development for accurate 16S rRNA gene amplicon sequencing
... Rapid analysis of tens or hundreds of independent specimens from medical patients or environmental samples will require a large number of technicians to collect, prepare, and analyse these ...the sequencing ...
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DISCOVERY OF POLYGALACTURONASE PRODUCING BACILLUS TEQUILENSIS STRAIN ARMATI USING 16S rRNA GENE SEQUENCING
... Methods: Poultry feces sample was serially diluted, and the pure isolate was subjected to morphological tests, biochemical tests, genomic DNA isolation, polymerase chain reaction amplification, amplicon purification and ...
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Identification of Bacterial Fish Pathogens in Brazil by Direct Colony PCR and 16S rRNA Gene Sequencing
... the sequencing of both methods exhibited Phred quality scores ...cost analysis per sample for direct colony PCR compared to Standard PCR of purified ...the genetic tools of the present ...
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Use of 16S rRNA Gene Sequencing for Rapid Identification and Differentiation of Burkholderia pseudomallei and B mallei
... seven genetic loci per sample, our approach is accom- plished with a simple DNA extraction and requires sequence data for only one gene (the 16S rRNA), making multilocus sequence typing more ...
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