genetic screen
Haploid Genetic Screen Reveals a Profound and Direct Dependence on Cholesterol for Hantavirus Membrane Fusion
... loss-of-function genetic screen in haploid human (HAP1) cells, as described previously ...The screen identi- fied seven genes that regulate cellular cholesterol metabolism ...
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A Genetic Screen for Modifiers of E2F in Drosophila melanogaster
... a genetic screen complexes is itself cell-cycle regulated (Sif et ...this screen is not saturated and that During this study we observed that GMR-dE2FdDP many additional E2F interactors remain to be ...
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A Genetic Screen for Hedgehog Targets Involved in the Maintenance of the Drosophila Anteroposterior Compartment Boundary
... The development of multicellular organisms requires the establishment of cell populations with different adhesion properties. In Drosophila, a cell-segregation mechanism underlies the maintenance of the anterior (A) and ...
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Genetic screen identifies a requirement for SMN in mRNA localisation within the Drosophila oocyte
... (Additional file 2: Table S2). The studies that have thus far explored a role for SMN in oogenesis have been few. Lee et al. [11] showed that defective nuclear organisa- tion was the most prominent early defect in SMN ...
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A Genetic Screen in Drosophila for Identifying Novel Components of the Hedgehog Signaling Pathway
... large-scale genetic screen in ...this screen we tested the ability of newly induced muta- for the expression of dpp and other low-threshold target tions to enhance or suppress a partial Hh ...
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A genetic screen for replication initiation defective (rid) mutants in Schizosaccharomyces pombe
... a genetic screen for cell division cycle (cdc) mutants that are sen- sitive to the loss of Chk1, but not ...This screen identified novel mutant alleles of cdc30/orp1 and ...
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A Chemical-Genetic Screen for Identifying Substrates of the Er Kinase Perk
... unbiased screen for PERK substrates (Cullinan et al, 2003). While this screen successfully identified the antioxidant response factor Nrf2, the list of PERK substrates has remained conspicuously ...
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An Unbiased Genetic Screen Reveals the Polygenic Nature of the Influenza Virus Anti-Interferon Response
... Most of the experimental evidence regarding how influenza virus circumvents the IFN response derives from studies of the pheno- type of NS1 deletion or point mutants and from protein-protein interaction studies involving ...
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A Genetic Screen Reveals that Synthesis of 1,4 Dihydroxy 2 Naphthoate (DHNA), but Not Full Length Menaquinone, Is Required for Listeria monocytogenes Cytosolic Survival
... novel genetic screen to identify ...the screen were attenuated in a murine model of ...our genetic screen uncovered factors required for ...
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Revitalization of a Forward Genetic Screen Identifies Three New Regulators of Fungal Secondary Metabolism in the Genus Aspergillus
... the genetic screen was designed, which relied on visual identification of NOR and discarded mutants with extreme developmental ...saturated genetic screen would have also discovered additional ...
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Targeted genetic screen in amyotrophic lateral sclerosis reveals novel genetic variants with synergistic effect on clinical phenotype
... We found that rare deleterious variants in G4C2-repeat-RNA binding partners act synergistically with C9ORF72 expansions to shorten disease duration. This is consistent with work from our group and others providing ...
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Targeted Genetic Screen in Amyotrophic Lateral Sclerosis Reveals Novel Genetic Variants with Synergistic Effect on Clinical Phenotype
... known genetic cause identified (n = 42) or young patients with sporadic ALS (n = 61) who are more likely to carry a pathogenic mutation than older patients with sporadic ALS (Cooper-Knock et ...
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A Genetic Screen for Temperature-Sensitive Cell-Division Mutants of Caenorhabditis elegans
... taken place, all three plates were examined for the presence Emb and Stu phenotypes were identified. Eighteen of of progeny males. For several strains, mating could not be these mutations were positioned on the ...
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A Genetic Screen to Identify Components of the sina Signaling Pathway in Drosophila Eye Development
... of genetic, molecular, and biochemical studies have led to identification of many of the signaling compo- nents required for R7 development, their biochemical functions, and their hierarchy within the R7 signaling ...
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A Genetic Screen of the Drosophila X Chromosome for Mutations That Modify Deformed Function
... our screen do not appreciably affect the formation of the gnathal cuticle, the functions of these genes might be supplied maternally or affect the development of Dfd-dependent tissues not visible in cuticular ...
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An Efficient Genetic Screen in Drosophila to Identify Nuclear-Encoded Genes With Mitochondrial Function
... low probability (56%) of localizing to the mitochon- drion. To determine the exact subcellular location of GatA, we constructed a GatA–GFP fusion protein, transfected Drosophila S2 cells with the construct, and ...
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Systematic Genetic Screen for Transcriptional Regulators of the Candida albicans White-Opaque Switch
... An initial screen of the 196 transcriptional regulator mutant strains was carried out to identify regulators with effects on white-to-opaque switching. The white-to-opaque switching rate was calculated by ...
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A Genetic Screen to Identify Genes Required for Drosophila Midline Development
... parallel genetic studies in vertebrate systems will facilitate our understanding the developmental roles of mam in both canonical and non-canonical Notch ...
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A Genetic Screen for Suppressors of Drosophila NSF2 Neuromuscular Junction Overgrowth
... Figure 6.—RT-PCR analysis of gene expression in GS2150 and GS3026. To determine which genes are regulated by the bidirectional GS elements identified in our screen, we used RT-PCR. (A and B) The genomic regions ...
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A Forward Genetic Screen in Mice Identifies Mutants with Abnormal Cortical Patterning
... Mutagenesis in mice using N-ethyl-N-nitrosourea (ENU) is a means to maximize the efficiency of a phenotype-based forward genetic analysis (Justice et al. 1999; Anderson 2000; Hatten and Heintz 2005; Stottmann and ...
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