Genome packaging
Complete and Incomplete Genome Packaging of Influenza A and B Viruses
... vRNA packaging into virions but could also reflect the uncoating and inefficient nuclear transport of RNPs or lack of vRNA transcription and/or viral protein synthesis, even though eight RNPs were packaged in ...
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The Genome-Packaging Signal of the Influenza A Virus Genome Comprises a Genome Incorporation Signal and a Genome-Bundling Signal
... he genome of influenza A virus is segmented into eight single- stranded negative-sense ...this genome segmenta- tion provides influenza viruses with a considerable evolutionary advantage through gene ...
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Creation of Rift Valley Fever Viruses with Four-Segmented Genomes Reveals Flexibility in Bunyavirus Genome Packaging
... random genome packaging ...three genome segments with identical UTRs in a single virion suggests a more random packaging mechanism, whereas the reduced growth and reduced virulence of the ...
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Deep Sequencing of Foot-and-Mouth Disease Virus Reveals RNA Sequences Involved in Genome Packaging
... viral genome, and such empty capsids also can be readily assembled in recombinant expression ...that packaging is specific for viral RNA, but the interactions driving packaging and conferring ...
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The Interaction of Bluetongue Virus VP6 and Genomic RNA Is Essential for Genome Packaging
... VP1 polymerase and capping enzyme VP4 are likely to be located beneath the VP3 layer at or near the 5-fold axis of icosahedral symmetry to facilitate the release of newly synthesized transcripts (6, 7). However, the ...
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Minimal cis-Acting Elements Required for Adenovirus Genome Packaging
... viral genome containing two HO sites was constructed in order to remove both ITRs in a ...viral genome, Ad- (Double HO), is shown in ...the packaging sequences as well as a second HO cleavage site in ...
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Paramyxovirus Ultrastructure and Genome Packaging: Cryo-Electron Tomography of Sendai Virus
... cases, we measured multiple fragments of nucleocapsid, allow- ing us to estimate the likely genome content. We found suffi- cient nucleocapsid in four of the virus particles investigated to suggest a polyploid ...
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Biophysics of V(D)J Recombination and Genome Packaging: In Singulo Studies on RAG, HMGB1, and TFAM
... V(D)J recombination is one of the most intriguing examples of the striking dynam- ics of genomes. The specific mechanistic workings of such genome dynamics still entail many unanswered questions. To address some ...
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Genome Segregation and Packaging Machinery in Acanthamoeba polyphaga Mimivirus Is Reminiscent of Bacterial Apparatus
... of genome replication, indi- cating their role in genome packaging (22, ...of genome packaging machinery in NCLDVs and virophages ...
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Assembly of Two Independent Populations of Flock House Virus Particles with Distinct RNA Packaging Characteristics in the Same Cell
... specific genome recognition on the ...specific genome packaging extended to particle assembly, the synthesis of coat proteins from both replicating and nonreplicating RNAs in the same cell would ...
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Human Cytomegalovirus pUL93 Is Required for Viral Genome Cleavage and Packaging
... viral genome cleavage and packaging and it could also be necessary for maintaining capsid stability after genome cleav- age and ...and packaging of viral DNA are believed to occur ...
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The Human Cytomegalovirus UL51 Protein Is Essential for Viral Genome Cleavage-Packaging and Interacts with the Terminase Subunits pUL56 and pUL89
... viral genome con- catemers were not cleaved after knockdown of pUL51 predicted that mature capsids are not generated in this ...ful genome packaging ...
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Surface Loop Dynamics in Adeno-Associated Virus Capsid Assembly
... The HI loop is a prominent domain on the adeno-associated virus (AAV) capsid surface that extends from each viral protein (VP) subunit overlapping the neighboring fivefold VP. Despite the highly conserved nature of the ...
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CLIP related methodologies and their application to retrovirology
... selective genome packaging ...HIV-1 genome in the cytosol revealed selective binding to sequences that coincide nearly precisely with a minimal element that can drive genome ...favors ...
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Conservation of a Packaging Signal and the Viral Genome RNA Packaging Mechanism in Alphavirus Evolution
... viral genome packaging ...the packaging signal, and more than one stem-loop is required for efficient genomic RNA ...for genome packaging, in the case of VEEV (and probably other ...
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Role of the Cytoplasmic Tail Domains of Bunyamwera Orthobunyavirus Glycoproteins Gn and Gc in Virus Assembly and Morphogenesis
... mini- genome (49). Packaging, as a surrogate for assembly, was mea- sured by the transfer of reporter gene activity (encoded by the minigenome) into cells “infected” with the ...
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Yellow Fever Virus NS3 Plays an Essential Role in Virus Assembly Independent of Its Known Enzymatic Functions
... engineered into YF23 and YF-Rluc2A-RP. YF23 containing W349F produced plaques with a wild-type phenotype, and W349Y produced small plaques, but none of the other substi- tutions produced detectable plaques, suggesting ...
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An Intact TAR Element and Cytoplasmic Localization Are Necessary for Efficient Packaging of Human Immunodeficiency Virus Type 1 Genomic RNA
... RNA packaging in human immunodeficiency virus (HIV) is lacking, it is widely believed that the HIV genome contains a packaging region that interacts with a viral factor to mediate the incor- poration ...
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High-Titer, Wild-Type Free Recombinant Adeno-Associated Virus Vector Production Using Intron-Containing Helper Plasmids
... icantly decrease rcAAV generation. There are only limited copies of rep and cap sequences in the cell line. But for the transfection-mediated rAAV production method, each cell usually receives thousands of copies of ...
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The L4 22-Kilodalton Protein Plays a Role in Packaging of the Adenovirus Genome
... The amber mutation that we introduced into the L4 22-kDa protein resulted in a lethal phenotype that appears to be due to a defect that occurs after late gene expression. This result is consistent with a role for the L4 ...
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