GenoType MTBDRplus
First Evaluation of GenoType MTBDRplus 2 0 Performed Directly on Respiratory Specimens in Central America
... GenoType MTBDRplus2.0 assay. The Genotype MTBDRplusversion 2.0 test (Hain Lifescience, Germany) is based on DNA strip technology, with three steps, i.e., DNA extraction, multiplex amplification with bio- ...
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EVALUATION OF THE GENOTYPE MTBDRPLUS ASSAY FOR RIFAMPIN AND ISONIAZID SUSCEPTIBILITY TESTING OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN SPUTUM SPECIME
... of Genotype MTBDRplus with phenotypic DST was ...the MTBDRplus DNA strip ...The Genotype MTBDRplus assay allows the rapid and specific detection of the most-frequent mutations leading ...
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Genotype MTBDRplus for Direct Detection of Mycobacterium tuberculosis and Drug Resistance in Strains from Gold Miners in South Africa
... GenoType MTBDRplus. Testing was conducted in accordance with the manufacturer’s recommendations (13) and the manufacturer-trained laboratory staff members. Training consisted of an initial 3 days of inten- ...
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GenoType MTBDRplus Assay for Molecular Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Strains and Clinical Samples
... the GenoType MTBDRplus assay (Hain Lifescience GmbH, Nehren, Germany) for its ability to detect resistance to rifampin (RIF) and isoniazid (INH) in Mycobacterium tuberculosis clinical strains and directly ...
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Evaluation of the GenoType MTBDRplus Assay for Rifampin and Isoniazid Susceptibility Testing of Mycobacterium tuberculosis Strains and Clinical Specimens
... the GenoType MTBDRplus assay is designed to detect mutations in the regulatory region of ...new GenoType MTBDRplus assay enhanced the rate of detection of INH resistance from 66 ...new ...
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Validation of the GenoType®MTBDRplus assay for diagnosis of multidrug resistant tuberculosis in South Vietnam
... and easy to perform for the simultaneous detection of rifampicin and INH resistance in M. tuberculosis. With high sensitivity for detection of rifampin resistance and high specificity for MDR, we conclude that this test ...
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Suitability of Xpert MTB/RIF and Genotype MTBDRplus for Patient Selection for a Tuberculosis Clinical Trial
... the GenoType MTBDRplus assay (MTBDR; Hain Lifescience GmbH, Nehren, Germany), for which mycobacterial DNA is amplified and reversely hy- bridized to detect Mycobacterium tuberculosis complex and common ...
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Evaluation of genotype MTBDRplus VER 2 0 line probe assay for the detection of MDR TB in smear positive and negative sputum samples
... sputum of patients who had treatment failure or relapse in a routine outpatient setting in the study. In addition, in the study done in South Africa by Barnard et al. [22] to evaluate LPA with Xpert MTB/RIF from smear ...
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Multicenter Noninferiority Evaluation of Hain GenoType MTBDRplus Version 2 and Nipro NTM+MDRTB Line Probe Assays for Detection of Rifampin and Isoniazid Resistance
... Hain GenoType MTBDRplus (referred to as Hain version 1 [V1]) for the rapid detection of rifampin (RIF) and isoniazid (INH) ...Hain Genotype MTBDRplus version 2 (referred to as Hain version 2 ...
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Performance Assessment of the GenoType MTBDRplus Test and DNA Sequencing in Detection of Multidrug Resistant Mycobacterium tuberculosis
... the GenoType MTBDRplus test and DNA sequencing, were assessed for the rapid detection of drug-resistant Mycobacterium tuberculosis for the first time in the Asia-Pacific ...the GenoType ...
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Diagnostic accuracy and usefulness of the Genotype MTBDRplus assay in diagnosing multidrug resistant tuberculosis in Cameroon: a cross sectional study
... The emergence of drug-resistant tuberculosis, espe- cially multidrug-resistant tuberculosis (MDR-TB), threatens TB control efforts in most countries including Cameroon. MDR-TB is caused by strains of Mycobac- terium ...
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The Diagnostic Performance of the GenoType MTBDRplus Version 2 Line Probe Assay Is Equivalent to That of the Xpert MTB/RIF Assay
... The most important advantage of the GenoType MTBDRplus (v2.0) assay is its enhanced sensitivity and the ability to determine INH susceptibility. These characteristics have the potential to im- prove the ...
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Validation of the GenoType® MTBDRplus assay for detection of MDR TB in a public health laboratory in Thailand
... Sputum specimens were processed using the U.S. Centers for Disease Control and Prevention (US CDC) recom- mended method of N-acetyl-L-cysteine 4% NaOH-2.9% citrate (final concentration of NaOH 1%). Following incu- bation ...
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Pathways to multidrug resistant tuberculosis diagnosis and treatment initiation: a qualitative comparison of patients’ experiences in the era of rapid molecular diagnostic tests
... Abstract Background: Although new molecular diagnostic tests such as GenoType MTBDRplus and Xpert® MTB/RIF have reduced multidrug-resistant tuberculosis MDR-TB treatment initiation times[r] ...
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Original Article Diagnostic performance of GenoType MTBDR plus on culture specimens in smear-negative retreatment tuberculosis patients
... MTB/RIF, GenoType MTBDRplus ...between GenoType MTBDRplus assay and phenotypic DST ...of GenoType MTBDRplus assay deserves an accu- rate and well-designed evaluation in retreat- ...
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Molecular detection of rifampin and isoniazid resistance to guide chronic TB patient management in Burkina Faso
... Methods: Samples were collected in Burkina Faso where culture and DST are not currently available, and where chronic cases are therefore classified and treated based on clinical evaluation and sputum-smear microscopy ...
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Drug resistance mutations and heteroresistance detected using the GenoType MTBDRplusassay and their implication for treatment outcomes in patients from Mumbai, India
... The GenoType MTBDRplus is superior in that it detects mutations associated with both rifampicin and isoniazid resistance unlike the INNO-LiPA ...the GenoType MTBDR plus assay has been reported in ...
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Evaluation of the GenoType MTBDR<em>plus</em> and MTBDR<em>sl</em> for the detection of drug-resistant <em>Mycobacterium tuberculosis</em> on isolates from Beijing, China
... As a rapid diagnosis of MDR-TB or even XDR-TB is of high importance for patient outcome and of high epidemio- logical importance, we evaluated detection performance of the GenoType MTBDRplus and MTBDRsl ...
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Utility of Second Generation Line Probe Assay (Hain MTBDRplus) Directly on 2 Month Sputum Specimens for Monitoring Tuberculosis Treatment Response
... The GenoType MTBDRplus line probe assay (LPA), version 2.0 (Hain Lifescience, Nehren, Germany), is a molecular diagnostic assay that has been widely used to identify resistance to first-line drugs rifampin ...
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Predictive Value of Molecular Drug Resistance Testing of Mycobacterium tuberculosis Isolates in Valle del Cauca, Colombia
... the GenoType MTBDRplus and MTBDRsl tests, which are already in use in multiple countries around the world but were only recently introduced in ...Beijing genotype among MDR-TB cases (10), and where ...
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