Genotyping - Fragment Length Polymorphism
High Resolution Genotyping of Streptococcus pyogenesSerotype M1 Isolates by Fluorescent Amplified Fragment Length Polymorphism Analysis
... amplified-fragment length polymorphism (FAFLP) analysis to subtype clinical isolates of Streptococcus pyogenes serotype ...amplified fragment differences. The high discriminatory power of ...
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Fluorescent Amplified Fragment Length Polymorphism Genotyping of Neisseria meningitidis Identifies Clones Associated with Invasive Disease
... amplified-fragment length polymorphism (FAFLP), a genotyping technique with phylogenetic significance, was applied to 123 isolates of Neisseria ...
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Sensitive PCR Restriction Fragment Length Polymorphism Assay for Detection and Genotyping of Giardia duodenalis in Human Feces
... PCR-restriction fragment length polymorphism analysis for the detection and genotyping of Giardia duodenalis on the basis of polymorphism in the triose phosphate isomerase (tpi) gene ...
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Fluorescent Amplified Fragment Length Polymorphism Genotyping of Campylobacter jejuni and Campylobacter coli Strains and Its Relationship with Host Specificity, Serotyping, and Phage Typing
... amplified fragment length polymorphism (FAFLP) analysis was applied to 276 Campylobacter jejuni strains and 87 Campylobacter coli strains isolated from humans, pigs, cattle, poultry, and retail meats ...
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Distribution Study of Chlamydia trachomatis Serovars among High Risk Women in China Performed Using PCR Restriction Fragment Length Polymorphism Genotyping
... Research & Training in Tropical Diseases (TDR), World Health Organization, 20 Avenue Appia, CH-1211 Geneva 27, Switzerland 2 ; and London School of Hygiene and Tropical Medicine, Keppel Street, London, United Kingdom 3 ...
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Comparative Genotyping of Campylobacter jejuni by Amplified Fragment Length Polymorphism, Multilocus Sequence Typing, and Short Repeat Sequencing: Strain Diversity, Host Range, and Recombination
... to genotyping by pulsed-field gel electrophore- sis and many ...molecular genotyping meth- ods may solve some of these ...amplified fragment length polymorphism (AFLP) analysis (3, 8, ...
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Genotyping Neisseria gonorrhoeaeUsing Fluorescent Amplified Fragment Length Polymorphism Analysis
... Elucidating relationships between isolates of Neisseria gon- orrhoeae can contribute to the identification of outbreaks and sexual networks, the recognition of temporally or geographi- cally predominant strains, and the ...
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Comparative Fingerprinting Analysis ofCampylobacter jejuni subsp jejuni Strains by Amplified Fragment Length Polymorphism Genotyping
... further increase the time and labor used versus those for the AFLP protocol. We observed that the PCR-RFLP method also offered good discrimination but, like PFGE, failed to separate strains from some of the different ...
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Genotyping by Amplified Fragment Length Polymorphism Analysis Reveals Persistence and Recurrence of Infection with Streptococcus anginosus Group Organisms
... The isolates were identified by the 16S rRNA reverse line blot hybridization assay as S. anginosus (n ⴝ 34), S. constellatus (n ⴝ 55), and S. intermedius (n ⴝ 8). Amplified fragment length ...
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Genotyping of Epidemic Methicillin ResistantStaphylococcus aureus Phage Type 15 Isolates by Fluorescent Amplified Fragment Length Polymorphism Analysis
... amplified-fragment length polymorphism (FAFLP) analysis was investigated for its ability to identify and subtype isolates of an epidemic methicillin-resistant phage type of Staphylococcus aureus, ...
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Improved Genotyping Vaccine and Wild Type Poliovirus Strains by Restriction Fragment Length Polymorphism Analysis: Clinical Diagnostic Implications
... A minimum number of four restriction enzymes, HaeIII, DdeI, NcoI and AvaI, was sufficient for complete inter- and intratypic differentiation of polioviruses. HpaII and StyI were also examined, however, to compensate for ...
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Comparison of Two Target Genes for Detection and Genotyping of Giardia lamblia in Human Feces by PCR and PCR Restriction Fragment Length Polymorphism
... Received 16 June 2005/Returned for modification 2 August 2005/Accepted 28 September 2005 A PCR assay targeting the tpi gene was developed to detect and to genotype Giardia lamblia in human feces. Our assay was specific ...
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Fluorescent Amplified Fragment Length Polymorphism Genotyping of Salmonella enterica subsp enterica Serovars and Comparison with Pulsed Field Gel Electrophoresis Typing
... We have performed the fluorescently labeled amplified-fragment length polymorphism (FAFLP) method on 97 strains of the food-borne pathogen Salmonella enterica subsp. enterica comprising seven ...
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Genotyping of Madurella mycetomatis by Selective Amplification of Restriction Fragments (Amplified Fragment Length Polymorphism) and Subtype Correlation with Geographical Origin and Lesion Size
... Institute of Endemic Diseases, and Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan 4 Received 11 February 2005/Returned for modification 31 March 2005/Accepted 2 June 2005 One of the ...
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Genotyping of Francisella tularensis Strains by Pulsed Field Gel Electrophoresis, Amplified Fragment Length Polymorphism Fingerprinting, and 16S rRNA Gene Sequencing
... Madrid, 3 Spain; Infectious and Parasitic Diseases Department, Armed Forces Institute of Pathology, Washington, D.C. 20306 2 ; and Institute for Veterinary Bacteriology, Bern, Switzerland 4 Received 12 December ...
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Evaluation of the Epidemiological Relevance of Variable Number Tandem Repeat Genotyping of Mycobacterium bovis and Comparison of the Method with IS6110 Restriction Fragment Length Polymorphism Analysis and Spoligotyping
... Spoligotypes were often distinguished by MIRU-VNTR typing. For instance, the single SB0162 spoligotype charac- terizing the highly prevalent RFLP group G corresponded to 5 distinct MIRU-VNTR types. In contrast, ...
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Genotyping of Candida orthopsilosis Clinical Isolates by Amplification Fragment Length Polymorphism Reveals Genetic Diversity among Independent Isolates and Strain Maintenance within Patients
... amplified fragment length polymorphism (AFLP) markers show indi- cation of size homoplasy and of a relationship between degree of homoplasy and fragment ...
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Fluorescent amplified fragment length polymorphism (FAFLP) genotyping demonstrates the role of biofilm-producing methicillin-resistant periocular Staphylococcus epidermidis strains in postoperative endophthalmitis
... increasing virulence. One recent study has showed that Fbe is a major factor involved in adherence of S. epider- midis to fibrinogen [11]. Antibodies against Fbe could block adherence of the bacteria to fibrinogen-coated ...
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Amplified Fragment Length Polymorphism Analysis: the State of an Art
... CONCLUSIONS AFLP analysis has established itself as a broadly applicable genotyping method with high degrees of reproducibility and discriminatory power. Several applications in taxonomy, diag- nostics, and ...
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Amplified Fragment Length Polymorphism Fingerprinting of Mycoplasma Species
... RESULTS Complexity of the AFLP fingerprints. In this study AFLP was used for genotyping a total of 50 Mycoplasma strains of human and animal origin (Table 1). The detection system was chosen to provide an optimal ...
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