Genotyping using High Resolution Melting (HRM)
Development of a Rapid High Throughput Method for High Resolution Melting Analysis for Routine Detection and Genotyping of Noroviruses
... rapid, high-throughput detection and genotyping method for noroviruses using real-time reverse transcription-PCR (RT-PCR) and high-resolution melting (HRM) analysis to create a ...
6
Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) through the implementation of a High Resolution Melting (HRM) genotyping assay
... to high-resolution melting (HRM) based on the amplification of two molecular markers — the divergent domain of the 24 s α rRNA gene and the intergenic region of the mini-exon ...the genotyping ...
6
Identification of Six New World Leishmania species through the implementation of a High Resolution Melting (HRM) genotyping assay
... Currently, the methods employed for the discrimination of New World Leishmania species are laborious, subject- ive and often lacking of specificity [21]. Therefore, it is crucial to count on a low-cost, effective and ...
7
Central line-associated bloodstream infection and application of high resolution melting to methicillin resistant Staphylococcus aureus and Shigella sonnei genotyping
... In the United States, the National Healthcare System Network (NHSN) was established in 2005 to collaborate with CDC on creation of infection surveillance systems using standardised methods and to categorise all ...
52
Rapid detection of Echinococcus species by a high resolution melting (HRM) approach
... Background: High-resolution melting (HRM) provides a low-cost, fast and sensitive scanning method that allows the detection of DNA sequence variations in a single step, which makes it appropriate for ...
5
Profiling of adulterant in tongkat ali herbal product using dna barcoding in combination with high resolution melting analysis
... as High Resolution Melting (HRM) analysis that will help to compliment the result of DNA ...the melting behaviour of double stranded DNA to single stranded DNA by capturing signals from ...
47
The Use of High Resolution Melting Analysis for Detection of the CAPN530 Polymorphism in Slovak Simmental Bulls
... The high-resolution melting analysis is a cost-effective and simple post-PCR technique that can be used for SNP ...the high resolution melting analysis for detection of SNP ...
5
High resolution melting analysis for molecular detection of multidrug resistance tuberculosis in Peruvian isolates
... days using clinical sam- ...the melting phase following real-time ...SNPs, genotyping, gene scanning, sequence matching and nucleic acids methylation ...analysis using Peruvian culture ...
6
Identification of Phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation
... the resolution of the analyses. Although our genotyping method is a promising approach to assign new unclassified and rapidly evolved Phy- tophthora strains to a specific species, this potential has not ...
6
High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes
... S: Genotyping of the protozoan pathogen toxoplaasma gondii using high-resolution melting analysis of the repeated B1 ...of high-resolution melting analysis for ...
5
High-Resolution Melting Analysis for Screening of Turkish Germline Mutations in BRCA1 and BRCA2
... PATIENTS AND METHODS Patients Fifty women with family history of breast and/or ovarian cancer in their first degree-relatives were included in this study. Twenty-five of them had al- so self history of breast cancer. ...
6
Rapid Detection of Isoniazid, Rifampin, and Ofloxacin Resistance in Mycobacterium tuberculosis Clinical Isolates Using High Resolution Melting Analysis
... DNA dye which binds to double-stranded but not single- stranded DNA and can detect heteroduplexes during homo- geneous melting curve analysis. It has successfully been used in HRMA due to its ability to saturate ...
8
A Guide to High Resolution Melting (HRM) Analysis
... During melting, only dye bound to dsDNA fluoresces, and that fluorescence is continuously detected by an optical ...is high when the dye is in its bound state, but when in solution, the dye changes ...
20
A High-Resolution Melting Approach for Analyzing Allelic Expression Dynamics
... varieties in their response to cold storage at the transcript abundance level. Discussion Sensitivity and reproducibility are crucial parameters for all analyses of gene expression. The improved reliability of the ...
10
Real-Time PCR, High Resolution Melting - aplicações forenses
... Como perspetivas futuras, por exemplo, a avaliação do mRNA através de PCR em tempo real é uma técnica que pode oferecer um nível de confiança e expandir o conhecimento alh[r] ...
93
High-Throughput High-Resolution Class I HLA Genotyping in East Africa.
... level resolution needed to interpret immune responses, lack sufficient throughput for large immunoepidemiological ...between high resolution and high ...PCR using sequence-specific ...
18
Evaluation of spa-typing of methicillin-resistant Staphylococcus aureus using high-resolution melting analysis
... Fifteen out of nineteen spa-types each had distinct Tm, which was sufficient to unambiguously type each of these spa-types. The remaining 4 spa-types cannot be separated by Tm alone: t008 and t2770 shared Tm (80.3 o C) ...
18
High-resolution DNA melting analysis for simple and efficient molecular diagnostics
... conditions. Genotyping Although there are many methods of genotyping, closed-tube methods have strong advantages for the clinical laboratory, point-of-care diagnostics and personalized ...
12
High Resolution Genotyping of Campylobacter Species by Use of PCR and High Throughput Mass Spectrometry
... a high resolution among the vast majority of STs in the database ...tional resolution, practical constraints associated with the use of a large number of primers kept the panel relatively ...
6
High-Resolution Mapping of Quantitative Trait Loci by Selective Recombinant Genotyping
... required genotyping data points can by the single point of recombination within the target be reduced even more by noting that, within the target segment for that ...with genotyping, it is possible to ...
10