germinal vesicle
Effects of cyclic adenosine monophosphate modulators on maturation and quality of vitrified-warmed germinal vesicle stage mouse oocytes
... Suppression of the oocyte cell cycle to GV state seems to be crucial to prevent cryodamage of DNA structure. The chromatin of the GV oocyte in the diplotene stage of pro- phase I is known to be less sensitive to ...
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Epigenetic reprogramming in somatic cells induced by extract from germinal vesicle stage pig oocytes
... from germinal vesicle (GV) stage oocytes improved the efficiency of producing cloned mouse offspring by the demethylation of histones in the donor somatic cell nucleus (Bui et ...
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Induction of germinal vesicle break down (gvbd) in vitro by metabolic hormones in frog (euphlyctis cyanophlyctis) oocytes
... All the glasswares used for the oocyte maturation assay were thoroughly cleaned autoclaved. Maturation of oocytes was studied in the batches of 10 oocytes in triplicate incubated in 1ml of medium in 24 well culture ...
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Eccentric position of the germinal vesicle and cortical flow during oocyte maturation specify the animal vegetal axis of ascidian embryos
... Translocation of the germinal vesicle by centrifugal force Fully grown oocytes with the GV were dissected from gonads during the spawning season. The oocytes of Halocynthia are relatively large (280 µm) and ...
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The cytoplasm of mouse germinal vesicle stage oocytes can enhance somatic cell nuclear reprogramming
... The success of somatic cell cloning gives hope to programs such as species conservation, livestock propagation and cell therapy for regenerative medicine (Wilmut et al., 1997; Wilmut et al., 2002). To date, almost all ...
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The Effects of cAMP-elevating Agents and Alpha Lipoic Acid on In Vitro Maturation of Mouse Germinal Vesicle Oocytes
... Methods: Mouse germinal vesicle (GV) oocytes were categorized into cumulus de- nuded oocytes (DOs; n=896) and cumulus oocyte complexes (COCs; n=1077) groups. GV oocytes were matured in vitro with or without ...
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The Effects of cumulus cells on in vitro maturation of mouse germinal vesicle stage oocytes
... Oocyte maturation is often conceptually divided into nuclear and cytoplasmic processes. Nuclear maturation is a term that refers to the resumption of meiosis from the germinal vesicle (GV) stage and ...
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Comparing the viability and in vitro maturation of cumulus germinal vesicle break down (GVBD) oocyte complexes using two vitrification techniques in mice
... patients with polycystic ovarian syndrome (3). Over one decade after the first pregnancy was achieved using frozen and thawed oocyte (4), Fabbri et al (2001) reported a modified slow freezing and rapid thawing method to ...
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Xenopus oocyte maturation: cytoplasm alkalization is involved in germinal vesicle migration
... IlIt, I, Dev,lIilll ~O ~71 ~76 (1996) Original Article Xenopus oocyte maturation cytoplasm alkalization IS involved in germinal vesicle migration STEPHANE FLAMENT', EDITH BROWAEYS', JEAN LUC RODEAU2,[.] ...
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Genome organization and epigenetic marks in mouse germinal vesicle oocytes
... In the past decade, several groups already analyzed histone modifications in fully-grown mouse oocytes. Whereas an intense staining for all acetylated lysine residues at histone H4 (K5; K8, K12 and K16) or H3 (K9, K14 ...
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Reconstruction of mammalian oocytes by germinal vesicle transfer: A systematic review
... for germinal vesicle stage oocytes to reconstruct the maturation of ...utilized germinal vesicle transfer techniques, fusion, and fertilization survival rate on five different mammalian ...
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Comparison of Gene Expression Profiles in Human Germinal Vesicle Before and After Cytoplasmic Transfer From Mature Oocytes in Iranian Infertile Couples
... Considering the successful transfer of cytoplasm from a mature oocyte to high-risk oocytes (due to cytoplasmic defects) in both animal and human studies (8-11), the[r] ...
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Inhibition of germinal vesicle breakdown using IBMX increases microRNA-21 in the porcine oocyte
... To characterize the effect of artificially increasing MIR21 on oocyte competence without inhibiting GVBD, a MIR21 mimic, scrambled microRNA negative control, or nuclease free water was m[r] ...
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SIT1 is a betaine/proline transporter that is activated in mouse eggs after fertilization and functions until the 2 cell stage
... Betaine ( N,N,N -trimethylglycine) added to culture media is known to substantially improve the development of preimplantation mouse embryos in vitro, and to be imported into 1-cell embryos by a transporter that also ...
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Occurrence of Intersex in Grey mullet, Mugil cephalus L. from Kovalam coast, Tamil Nadu
... the reproductive system into male and female components. But it showed the occurrence of both oocytes and spermatozoa throughout the entire region of the testis. With reference the occurrence of oocytes, as many as five ...
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MOLECULAR CLONING OF α-AMYLASE GENES FROM DROSOPHILA MELANOGASTER. II. CLONE ORGANIZATION AND VERIFICATION
... The presence of functional a-amylase structural genes in XDm65 was verified by injection of appropriate subclones into the germinal vesicle of Xenopus oocytes, followe[r] ...
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FORMULATION, DEVELOPMENT AND CHARACTERIZATION OF ETHOSOMES OF ATORVASTATIN
... separate beaker was added to the mixture which was then stirred for 5 min in a covered vessel using magnetic stirrer. The vesicle size of ethosomal formulation was then reduced by ultrasonic probe sonicator for 10 ...
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An investigation into the regulated secretory pathway of granulocytes
... synaptic vesicle fusion complex were ...synaptic vesicle components, but other cell types undergoing regulated secretion do not contain comparable numbers o f vesicles or granules undergoing regulated ...
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Responsive Polydiacetylene Vesicles for Biosensing Microorganisms
... 2) Other types of responsive vesicles are based on the vesicle encapsulation method: fluorescent 184.. dye or redox species being encapsulated in the vesicle.[r] ...
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Elastic liposomes mediated transdermal delivery of verapamil hydrochloride
... as vesicle shape, vesicle size and size distribution, entrapment efficiency, elasticity measurements, stability studies and in vitro skin permeation studies through excised rat skin (Sprague Dawley) using a ...
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