GFP-Fusion
A toolkit for GFP mediated tissue specific protein degradation in C elegans
... the GFP-tagged protein by the Cul2 family E3 ligase CUL-2 and its subsequent degradation by the proteasome (DeRenzo et ...a GFP nanobody::ZIF-1 fusion (epiDEG; ...tagged GFP fusion with ...
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Adaptive Mutations in the Nuclear Export Protein of Human-Derived H5N1 Strains Facilitate a Polymerase Activity-Enhancing Conformation
... for GFP fused to Borna disease virus phosphoprotein P (GFP-P) were set to ...the GFP fusion proteins were determined by Western blotting using GFP-specific ...indicated GFP ...
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Immobilisation of active enzymes on novel GFP protein particles : a thesis submitted in complete fulfilment of the requirements of the degree of Master of Science in Microbiology at Massey University, Palmerston North, New Zealand
... for GFP fluorescence. GFP fluorescence is generated by a hexapeptide chromophore consisting of the amino acids Phe64-Ser65-Tyr66-Gly67-Val68-Gln69 with autocatalytic cyclisation occurring between ...
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Polycomb group protein complexes exchange rapidly in living Drosophila
... on GFP fusion proteins of Polycomb (Pc) and Polyhomeotic (Ph), two essential members of the PCC in whole living Drosophila embryos and larval tissues to determine their diffusion, binding equilibria and ...
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Human Cytomegalovirus UL97 Kinase Activity Is Required for the Hyperphosphorylation of Retinoblastoma Protein and Inhibits the Formation of Nuclear Aggresomes
... hypothesis, GFP-GCP170* was co- expressed with pp71 and pUL69 in COS7 cells to see whether they exhibited similar staining ...with GFP-GCP170*, suggesting that FIG. 2. Inhibition of pp71 and ...
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Spectral properties of LH2 exhibit very similar even when heterologously express LH2 with β subunit fusion protein in Rhodobacter sphaeroides
... β-subunit- GFP fusion protein in ...β-subunit-GFP fusion pro- tein complexes still possessed normal activity in en- ergy ...β-subunit-GFP fusion protein complexes com- pared to ...
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A 38-Amino-Acid Sequence Encompassing the Arm Domain of the Cucumber Necrosis Virus Coat Protein Functions as a Chloroplast Transit Peptide in Infected Plants
... CP GFP fusion ...the GFP fusion ...The GFP in these constructs [with the exception of GFP/arm( ⫹ SVRI)/CNVexp] was His tagged at the carboxyl terminus ...of fusion ...
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Subcellular Localization of Transmembrane E cadherin GFP Fusion Protein and Expression in HeLa Cells
... Colony PCR was performed to investigate the insert orientation by using two primer combinations (Gene specific Forward primer + GFP Reverse primer and Gene specific Reverse primer + GFP Reverse primer). Two ...
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Role of Kaposi's Sarcoma-Associated Herpesvirus C-Terminal LANA Chromosome Binding in Episome Persistence
... expressing GFP NLS or GFP LANA 933–1162 ...of GFP (green) and chromosomes (red) generates ...expressing GFP NLS, GFP LANA 933–1162, or GFP LANA 933–1162 mutants were arrested in ...
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Evaluation of VP22 Spread in Tissue Culture
... the GFP fusion ...affecting GFP fluo- rescence of the fusion protein, this might be directly demon- strated by testing the ability of PFA to quench the intrinsic GFP fluorescence that ...
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ATP-Dependent Localization of the Herpes Simplex Virus Capsid Protein VP26 to Sites of Procapsid Maturation
... encoding GFP (3) was fused to the amino-terminal coding region of the VP26 gene under the control of the constitutive cytomegalovirus immediate-early promoter ...VP26-GFP fusion protein of the ...
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Engrailed homeoprotein acts as a signaling molecule in the developing fly
... Fig. 1. En expression and crossvein formation. (A) MS1096-Gal4;+ adult Drosophila wings (left) show a normal pattern of veins, with longitudinal veins (L1 to L5) and anterior (ACV) and posterior (PCV) crossveins. In en ...
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Conditional Cytomegalovirus Replication In Vitro and In Vivo
... virus, GFP-1, and SCP-GFP-2 revealed no differ- ence in growth on MEF under multistep growth conditions (MOI of ...SCP- GFP-2 was reduced by a factor of 1,000 in response to ...
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Inhibition of Akt activity and calcium channel function coordinately drive cell cell fusion in the BeWO choriocarcinoma placental cell line
... cell fusion, we took advantage of thapsigargin, a sesquiterpene lactone that raises cytosolic calcium concentration by blocking the calcium pump of the endoplasmic reticulum ...forskolin-induced fusion of ...
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Insertion of Green Fluorescent Protein into Nonstructural Protein 5A Allows Direct Visualization of Functional Hepatitis C Virus Replication Complexes
... the GFP insert. GFP is retained in NS5A during RNA ...NS5A-GFP fusion protein was stable during HCV RNA ...NS5A-GFP fusion protein, was detected in I/5A-GFP-6 cells by ...
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Secretion and expression dynamics of a GFP tagged mucin type fusion protein in high cell density Pichia pastoris bioreactor cultivations
... mucin-type fusion protein by Pichia pastoris in 1 L scale ...the fusion protein in terms of intracellular- and extracellular concentra- ...the fusion protein synthesis rate. To track the ...
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Characterization of the Expression, Intracellular Localization, and Replication Complex Association of the Putative Mouse Hepatitis Virus RNA-Dependent RNA Polymerase
... A proposed model for the formation of the replication com- plex during virus infection would be the anchoring of the 150- kDa precursor to membranes via MP1 and MP2, with contin- ued cotranslational and posttranslational ...
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Intracellular Localization of ABC Transporter TAPL Differs between Transient and Stable Expression
... with GFP under transient expression could become targets of quality con- trol system(s) of ER [20] and that the exit of TAPL molecules from ER to lysosomes through Golgi apparatus could be ...
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Farnesyl diphosphate synthase, the target for nitrogen-containing bisphosphonate drugs, is a peroxisomal enzyme in the model system Dictyostelium discoideum
... DdFDPS–GFP fusion proteins lacking a functional PTS2 were able to grow in concentrations of alendronate that inhibited growth of untransformed ...these fusion proteins were catalytically active and, ...
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Virion stiffness regulates immature HIV 1 entry
... membrane fusion during entry, particle disassembly to release genetic material, assembly during budding, and stability in the extracellu- lar environment before entry into the next ...
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