gH/gL
Human Cytomegalovirus gH/gL/gO Promotes the Fusion Step of Entry into All Cell Types, whereas gH/gL/UL128-131 Broadens Virus Tropism through a Distinct Mechanism
... mostly gH/gL/gO (de- noted Merlin-trimer [ME-T]) (25, ...of gH/gL complexes among HCMV strains, extracellular virions of TB, TR, ME, and ME-T (ME virions produced in TetR- expressing cells) ...
11
N-Terminal Mutants of Herpes Simplex Virus Type 2 gH Are Transported without gL but Require gL for Function
... with gL, gB, and, in most alphaherpesviruses, gD. Within the gH/gL heterodimer, it is thought that gH accounts for the fusion function and gL acts as a chaperone for the folding and ...
11
Structural and Antigenic Analysis of a Truncated Form of the Herpes Simplex Virus Glycoprotein gH-gL Complex
... and gL were each detected in column fractions by ...and gL eluted from the column in a single peak ...and gL can also exist as higher-molecular-mass forms, ...of gL was found in the absence of ...
12
Glycoprotein gL-Independent Infectivity of Pseudorabies Virus Is Mediated by a gD-gH Fusion Protein
... The gH-gL complex is conserved throughout the herpesvi- ruses, although the nature of the linkage between the two components may ...that gH requires gL for proper folding and intracellular ...
9
Mutations in Pseudorabies Virus Glycoproteins gB, gD, and gH Functionally Compensate for the Absence of gL
... In the alphaherpesviruses herpes simplex virus 1 (HSV-1) and pseudorabies virus (PrV), the cascade ultimately leading to mem- brane fusion is initiated by interaction of the essential viral attach- ment glycoprotein gD ...
9
Extreme Dependence of gH and gL Expression on ORF57 and Association with Highly Unusual Codon Usage in Rhesus Monkey Rhadinovirus
... of gH (A) or gL (B) RNA, the blot was stripped and reprobed for cyclophilin A as a control for equal RNA ...(Invitrogen), gH, c.o. gH, gL, or c.o. gL are indicated by ...
7
Epstein-Barr virus glycoprotein gH/gL antibodies complement IgA-viral capsid antigen for diagnosis of nasopharyngeal carcinoma
... EBV gH/gL. The construction of soluble EBV gH/gL has been described previously ...constructs, gH (aa 18-679) [GenBank: NC009334] and gL (aa 24-137) [GenBank: YP001129472] were ...
12
The Human Cytomegalovirus UL116 Gene Encodes an Envelope Glycoprotein Forming a Complex with gH Independently from gL
... the gH/gL scaffold: the trimeric gH/gL/gO and the pentameric gH/gL/UL128/UL130/UL131A complex ...of gH/gL with these alternative complexes relies on the formation ...
13
A Functional Interaction between Herpes Simplex Virus 1 Glycoprotein gH/gL Domains I and II and gD Is Defined by Using Alphaherpesvirus gH and gL Chimeras
... a gH/gL ...nor gH/gL functioned with heterotypic entry glycoproteins, indicating that gD and gH/gL exhibit an essential type-specific functional ...on gH/gL, we ...
11
Mutations in the Amino Terminus of Herpes Simplex Virus Type 1 gL Can Reduce Cell-Cell Fusion without Affecting gH/gL Trafficking
... the gH/gL heterodimer for gL residues that are exposed or project outward, we identified five gH residues that appeared to have side chains projecting outward from the top or crown of H1A in a ...
6
Human Herpesvirus 8 Glycoprotein B (gB), gH, and gL Can Mediate Cell Fusion
... and gH-gL was more ...and gH-gL can induce the fusion of CHO and B ...gB, gH, and gL to mediate the fusion of CHO cells with other cell ...gB, gH, and gL could ...
11
The Large Groove Found in the gH/gL Structure Is an Important Functional Domain for Epstein-Barr Virus Fusion
... in gH/gL by gp42 may be different from that which leads to epithelial cell fusion, since the flexibility between D-I and D-II can be ...a gH/gL conformational change is trig- gered by ...
8
Intracellular Formation and Processing of the Heterotrimeric gH-gL-gO (gCIII) Glycoprotein Envelope Complex of Human Cytomegalovirus
... to gH. Figure 3B shows that gH and a 115-kDa gH-gL dimer (15) were evident in the pulse ...of gH, gL, and the 100- kDa form of ...when gH, gL, and the 100-kDa gO ...
7
Characterization of the Human Cytomegalovirus gH/gL/UL128-131 Complex That Mediates Entry into Epithelial and Endothelial Cells
... glycoprotein gH/gL. gH/gL/UL128-131 complexes appear to be distinct from the gH/gL/gO complex, which likely facilitates entry into ...of gH/gL/ UL128-131 complexes, ...
11
Importance of Highly Conserved Peptide Sites of Human Cytomegalovirus gO for Formation of the gH/gL/gO Complex
... 4CtoA_C343intact, gH/gL/gO was detectable only in the mutant with an intact C343, whereas none of the other cysteines could individually rescue complex ...no gH/gL/gO was detected in lysates ...
22
Using Antibodies and Mutants To Localize the Presumptive gH/gL Binding Site on Herpes Simplex Virus gD
... activate gH/gL. Even though an interaction between gD and gH/gL has been shown by bimolecular com- plementation (44, 45), its biological significance has not been established, as the enhanced ...
15
Kaposi's Sarcoma-Associated Herpesvirus gH/gL: Glycoprotein Export and Interaction with Cellular Receptors
... for gH ⌬ TM-Fc, although binding was abolished only by complete desulfation of heparin residues ...1. gH is expressed at the cell surface independently of ...for gH-Flag, gL-myc, or ...only. ...
12
Monoclonal Antibodies to Different Components of the Human Cytomegalovirus (HCMV) Pentamer gH/gL/pUL128L and Trimer gH/gL/gO as well as Antibodies Elicited during Primary HCMV Infection Prevent Epithelial Cell Syncytium Formation
... where gH/gL plus gB is overexpressed by use of ectopic ...of gH MAbs) are consistent with the idea that the gH/gL/gO complex is involved in entry in all cell types, probably by promot- ...
8
Comparative Analysis of gO Isoforms Reveals that Strains of Human Cytomegalovirus Differ in the Ratio of gH/gL/gO and gH/gL/UL128-131 in the Virion Envelope
... of gL, and it is interesting that the anti-gL antibodies used were also raised against synthetic ...intact gH/gL/gO ...disulfide-linked gH/gL/gO complexes are a conserved feature ...
11
Scanning Mutagenesis of Human Cytomegalovirus Glycoprotein gH/gL
... the gH/gL/UL128-131 complex ...of gH/gL/gO and gH/gL/UL128-131 (19). The amounts of gH/gL/gO and gH/gL/UL128-131 in the virion envelope affect the ...
12