?-globin
Globin synthesis in fractionated Normoblasts of beta thalassemia heterozygotes
... Globin chain synthesis was examined in erythroid cells of increasing maturity, fractionated from the whole bone marrow of beta-thalassemia heterozygotes by a density gradient centrifugation procedure. In ...
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Understanding globin regulation in β thalassemia: it’s as simple as α, β, γ, δ
... human globin loci are among the best characterized in the human genome at the gene and protein lev- ...the globin structural genes — is a strong enhancer of the expression of the down- stream structural ...
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Design of retrovirus vectors for transfer and expression of the human beta-globin gene.
... Retrovirus vectors currently offer the best vehicle for gene transfer into marrow cells, and vectors carrying the P-globin gene have been used to transfer the ,-globin gene into murine e[r] ...
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Original Article β-thalassemia for translating human β-globin gene and RNA interference of α-globin gene expression by lentivirus vectors
... more, the excessive α-globin chain deposits together to form inclusion bodies, and the lifespan of red blood cells are negatively related to the number of their inclusion body. When the inclusion bod- ies are ...
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Spatial organization of the chicken beta-globin gene domain in erythroid cells of embryonic and adult lineages
... addition to the LCR, the domain also possesses an in- ternal enhancer (β/ε-enhancer), which is located be- tween the genes β A and ε and is believed to contribute to the control of the activity of both genes [12-14]. The ...
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Activation of the human beta globin promoter in K562 cells by DNA sequences 5' to the fetal gamma or embryonic zeta globin genes
... consistent with the known inactivity of the beta-globin gene in these cells. In contrast, a gamma/beta promoter composed of a gamma fragment spanning positions -408 to -137 joined to the 127-bp beta promoter was ...
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Globin gene expression in erythroid human fetal liver cells
... human globin mRNAs in liver samples from several mid-gestational ...alpha globin genes were present in fetal liver samples isolated from 10-25-wk ...human globin mRNAs declined in older fetuses, ...
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Locus assignment of alpha globin structural mutations by hybrid selected translation
... alpha- globin mutations can be fractionated into beta-, alpha 9 (total)-, alpha 1-, and alpha 2-globin mRNA by selective hybridization of each mRNA species to its respective complementary DNA (cDNA) ...
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DISTRIBUTION AND FATE OF INTRAVENOUSLY ADMINISTERED MODIFIED HUMAN GLOBIN AND ITS EFFECT ON BLOOD VOLUME STUDIES UTILIZING I131 TAGGED GLOBIN
... VENOUS PLASMA CONCENTRATIONS OF UNTAGGED INTERRUPTED LINE AND TAGGED GLOBIN FOLLOWING INTRAVENOUS ADMINISTRATION OF GLOBULIN-Im AT DIFFERENT CARRIER LEVELS AND OF TAGGED ALBUMIN AFTER A [r] ...
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Synthesis of Globin Chains in Sickle β Thalassemia
... for globin synthesis due to the nonthalassemic and thalassemic alleles in patients with sickle ...unbalanced globin synthesis in the peripheral blood of these patients is explained by the decrease in ...
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Platypus globin genes and flanking loci suggest a new insertional model for beta-globin evolution in birds and mammals
... We consider that a more parsimonious explanation is that the platypus ε is orthologous to the marsupial and euthe- rian embryonic β-like globin lineages (ε and γ), and arose by duplication of an ancestral ...
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Preferential Binding of βS Globin Chains Associated with Stroma in Sickle Cell Disorders
... If preferential binding of Pi chains to tive amounts of labeled globin chains present in the occurs during early erythroid cell development, supernate with globin chains associated with [r] ...
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Phylogenetic analysis reveals wide distribution of globin X
... invertebrate globin proteins and a partial GbX sequence of the green anole lizard [GenPept: ...in globin helix B), ...the globin domain (on average the first 57 amino acids), completely in the lizard ...
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An initiation codon mutation (AUG GUG) of the human alpha 1 globin gene Structural characterization and evidence for a mild thalassemic phenotype
... alpha-globin is encoded by two adjacent genes, alpha 1 and alpha 2. Recent evidence suggests that these genes are not equally expressed and that the alpha 2-globin gene encodes the majority of ...
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Translation and stability of human globin mRNA in Xenopus oocytes
... poly-(A)-rich globin mRNA is sustained for a longer period than that of the poly-(A)-poor ...synthesized globin decreases as the injected oocytes are incubated for longer ...
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Heterogeneity of DNA fragments associated with the sickle globin gene
... We have examined the genetic polymorphism previously reported to be associated with the sickle-cell (beta s) gene. The polymorphism involves an alteration of the DNA sequence 3' to the beta-globin gene as detected ...
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Phylogenetic Analysis of Globin Like Superfamily at Molecular Level
... of globin proteins among various species were performed by Neighbour Joining method in MEGA 7 to know evolutionary history of Truncated hemoglobin nucleotides among various ...
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Two missense mutations in the beta globin gene can cause severe beta thalassemia Hemoglobin Medicine Lake (beta 32[B14]leucine >glutamine; 98 [FG5] valine >methionine)
... beta- globin gene of the proband revealed two mutations, one for the moderately unstable hemoglobin (Hb) Köln and another for a novel codon 32 cytosine-thymidine-guanine-- >cytosine-adenine-guanine transversion ...
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Structural determinants of human ζ-globin mRNA stability
... α globin [27], β globin [16], α(I) collagen [28], tyrosine hydroxylase [29], histone [30], and the transferrin receptor [31]–require the assembly of defined mRNP effector com- plexes on specific ...
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alpha Thalassemia caused by an unstable alpha globin mutant
... mutation (CTG to CCG) in one of the genes resulted in a leucine to proline substitution. This paper describes the approach we used to detect the abnormal alpha-globin chain. The chain was identified using a ...
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