Glutamate decarboxylase
Contribution of glutamate decarboxylase in Lactobacillus reuteri to acid resistance and persistence in sourdough fermentation
... for glutamate metabolism in ...to glutamate decarbox- ylase (gadB), glutamate: g – aminobutyric acid (GABA) antiporters (gadC1 and gadC2), a glutaminase (gls3), and a xanthine/uracil/vitamin C ...
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Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum by expressing glutamate decarboxylase active in expanded pH range
... of glutamate into GABA by glutamate decarboxylase (GAD; EC ...of glutamate monosodium salt (MSG) or glutamic acid to ...brevis glutamate decarboxylase successfully produced GABA ...
11
Islet cell cytoplasmic autoantibody reactivity to glutamate decarboxylase in insulin dependent diabetes
... Abbreviations used in this paper: GAD, glutamate decarboxylase; IAA, insulin autoantibodyies; ICA, islet cell cytoplasmic autoantibody; IDD, insulin-dependent diabetes; JDF, Juvenile Dia[r] ...
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Exploring the contributions of two glutamate decarboxylase isozymes in Lactobacillus brevis to acid resistance and γ-aminobutyric acid production
... both glutamate decarboxylase and the antiporter are essential for acid ...confer glutamate- dependent acid resistance to this LAB, with GadA playing a minor ...
14
Effects of Jobelyn® on Isoniazid-Induced Seizures, Biomarkers of Oxidative Stress and Glutamate Decarboxylase Activity in Mice
... of glutamate decarboxylase, the enzyme that control the formation of GABA, a chemical that reduce the firing rate of nerve cells in the ...mate decarboxylase, which indicates the presence of more ...
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Detection of pancreatic islet 64,000 M(r) autoantigens in insulin dependent diabetes distinct from glutamate decarboxylase
... Patients with insulin-dependent diabetes (IDDM) possess antibodies to islet proteins of M(r)-64,000. Potential autoantigens of this M(r) include glutamate decarboxylase (GAD) and 65 kD heat shock protein. ...
10
Autoimmunity to two forms of glutamate decarboxylase in insulin dependent diabetes mellitus
... Insulin-dependent diabetes mellitus (IDDM) is thought to result from the autoimmune destruction of the insulin-producing beta cells of the pancreas. Years before IDDM symptoms appear, we can detect autoantibodies to one ...
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Comprehensive evaluation and selection of the potential complex medium for industrial glutamate decarboxylase (GAD) production by Escherichia coli
... enzyme, glutamate decarboxylase (GAD), this study evaluated the performance of the currently used complex and defined culture media in terms of biomass production, GAD yield, and byproducts accumulation, ...
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Comprehensive evaluation and selection of the potential complex medium for industrial glutamate decarboxylase (GAD) production by Escherichia coli
... enzyme, glutamate decarboxylase (GAD), this study evaluated the performance of the currently used complex and defined culture media in terms of biomass production, GAD yield, and byproducts accumulation, ...
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Extracellular expression of glutamate decarboxylase B in Escherichia coli to improve gamma-aminobutyric acid production
... importance, glutamate decarboxylase has been overexpressed in various bacteria to enhance GABA ...overexpressing glutamate decar- boxylase in ...horikoshii glutamate decarboxylase in ...
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Mechanistic studies on glutamate decarboxylase and serine hydroxmethyltransferase
... The first active-site amino acid to be identified was the lysine residue which forms an internal Schiff’s base complex with PLP. The reduction of the imine with sodium borohydrlde followed by acid hydrolysis of the ...
257
Assessment of autoantibodies to glutamate decarboxylase and anti-insulin in spontaneous diabetic dogs and experimental diabetic rat model
... (glutamate decarboxylase), IA-2 and insulin, autoantibodies are most widely used as predictive markers for insulin-dependent diabetes mellitus (IDDM) (Bingley, 2010; Wenzlau et ...patients, glutamate ...
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Demonstration of GAD 65 as the main immunogenic isoform of glutamate decarboxylase in type 1 diabetes and determination of autoantibodies using a radioligand produced by eukaryotic expression
... Plasmids containing cDNA for the rat 67- and 65-kD isoforms of glutamate decarboxylase (GAD-67 and GAD-65) were expressed in COS-cells, and lysates of [35S]methionine- labeled cells were used for ...
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A comparative study of extraction techniques for maximum recovery of glutamate decarboxylase (GAD) from Aspergillus oryzae NSK
... Glutamate decarboxylase (GAD) is a unique enzyme that catalyzes the irreversible conversion of L-glutamic acid (Glu) to γ-aminobutyric acid (GABA) and carbon diox- ide through a single step ...
9
Disruption of pknG enhances production of gamma-aminobutyric acid by Corynebacterium glutamicum expressing glutamate decarboxylase
... coli glutamate decarboxylase (GAD) B encoded by ...precursor glutamate, we focused on engineering pknG (encoding serine/threonine protein kinase G), which controls the activity of 2-oxoglutarate ...
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Monoclonal antibodies to 65kDa glutamate decarboxylase induce epitope specific effects on motor and cognitive functions in rats
... pressing GABAergic neurotransmission [12,13]. We in- vestigated the effect of GAD65Ab on neuronal activity by injecting rat cerebella with IgG purified from GAD65Ab-positive SPS and CA patients [14]. The anti- bodies ...
10
Cellular immunity to a determinant common to glutamate decarboxylase and coxsackie virus in insulin dependent diabetes
... However, the major determinant of GAD recognized by persons at increased risk for IDD was amino acids 247-279, a region which has significant sequence similarity to the P2-C protein of C[r] ...
6
Prevalence of autoantibodies to the 65 and 67 kD isoforms of glutamate decarboxylase in insulin dependent diabetes mellitus
... Autoantibodies to glutamic acid decarboxylase GAD detected by an immunotrapping enzyme activity assay: relation to insulin-dependent diabetes mellitus and islet cell antibodies.. Glutami[r] ...
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Homozygosity for a missense mutation in the 67 kDa isoform of glutamate decarboxylase in a family with autosomal recessive spastic cerebral palsy: parallels with Stiff-Person Syndrome and other movement disorders
... excitability of the motor cortex in SPS has been demon- strated by transcranial magnetic stimulation [39]. Anti-GAD 65 auto-antibodies in the CSF of ataxic and SPS patients selectively suppress GABA-mediated transmission ...
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Glutamate decarboxylase , insulin , and islet cell antibodies and HLA typing to detect diabetes in a general population based study of Swedish children
... Predictive value of islet cell and insulin autoantibodies for Type I diabetes mellitus in a population based study of newly diagnosed diabetic and matched control children.. High genetic[r] ...
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