GPI-anchored membrane protein
GPI-anchor and GPI-anchored protein expression in PMM2-CDG patients
... polypeptide- anchored form (FcγRIIIA, or CD16-A) in human natural killer cells and monocyte/macrophages, and as a GPI- anchored form in human neutrophils (FcγRIIIB or CD16- B) ...the membrane ...
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Oral Delivery of Protein Drugs: Driver for Personalized Medicine?
... the GPI anchor and produced by recombinant technologies was used as model ...This GPI-anchored insulin was labelled with immunogold to enable visualization by electron ...the GPI- ...
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Physical and biochemical characterisation of leucocyte membrane complexes containing GP1-anchored proteins
... the protein molecule in the cytoplasmic leaflet of the m em brane (Buser, et ...ith GPI-anchored proteins has been investigated particularly w ith reference to their association w ith ...
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Fate of Glycosylphosphatidylinositol (GPI)-Less Procyclin and Characterization of Sialylated Non-GPI-Anchored Surface Coat Molecules of Procyclic-Form Trypanosoma brucei
... integral membrane proteins that span the bilayer several times, and the putative single-pass transmembrane procyclic-form specific an- tigen PSSA-2 (15) was noticeably absent (see ...multispanning membrane ...
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The Role of GPI-anchored PrPC in Mediating the Neurotoxic Effect of Scrapie Prions in Neurons
... cell membrane and is involved in prion conversion extra- neuronally, these mice have rather low levels of mutant PrP ...actual protein appear lower still (see Chesebro et al; 2005, Figure ...
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Insight into the Antiadhesive Effect of Yeast Wall Protein 1 of Candida albicans
... a GPI-anchored glycoprotein of the ...the protein itself (10, 23, ...a GPI moiety an- chored in the plasma membrane. The GPI anchor is subsequently cleaved, and the 378-aa core ...
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GPAA1 promotes gastric cancer progression via upregulation of GPI-anchored protein and enhancement of ERBB signalling pathway
... functions, GPI-APs contribute to signal transduction through con- structing a platform, the lipid raft, to facilitate interac- tions among different proteins and ...cell membrane is heterogeneous, featuring ...
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Self assembling functional programmable protein array for studying protein–protein interactions in malaria parasites
... plasma membrane during invasion and some of them are involved in weak, low-affinity interac- tions with receptors on RBC membrane for selecting a specific target cell [3, ...are ...
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Human LYPD8 protein inhibits motility of flagellated bacteria
... hLYPD8 protein was observed at about the 75-kDa position ...N-glycosylated protein, like ...cleaves GPI-anchored proteins from the cell membrane, and analyzed hLYPD8 surface expression ...
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Release of exosomes and microvesicles harbouring specific RNAs and glycosylphosphatidylinositol anchored proteins from rat and human adipocytes is controlled by histone methylation
... the protein constitu- ents, recently reported data suggest that several different types of plasma membrane anchors and signals manage to direct highly oligomeric cytoplasmic polypeptides to the site(s) of ...
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Identification of ω-sites of Glycosylphosphatidylinositol Anchored Proteins
... outer membrane proteins contain a covalently linked glycosylphosphatidylinositol anchor (GPI-anchor), which tethers the protein to the extracellular face of the plasma ...common GPI core ...
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Development of a novel PIG-A gene mutation assay based on a GPI-anchored fluorescent protein sensor
... specifically, GPI-anchored proteins are first syn- thesized as ...C-terminal GPI anchor signal sequence (C-SP) is also cleaved, and the resulting mature protein is attached to the ...
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Endocytosis of glycosylphosphatidylinositol-anchored proteins
... basal membrane of HEK293 cells ...basal membrane with the dimeric form prominent in the basal membrane ...of GPI-APs with other membrane resident proteins and their influence on ...
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Human placental inositolphosphoglycans may be released by glycosylphosphatidylinositol-phospholipase D, both present in the syncytial brush border membrane investigation in normal and pre-eclamptic placentae
... plasma membrane. This microvillous membrane, which forms the surface of contact between the placenta and the maternal blood, can be easily isolated from the rest of the placenta using a method first ...
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The role of Gpi-anchored axonal glycoproteins in neural development and neurological disorders.
... found anchored to the membrane via a typical transmembrane domain, or via a Glycosyl Phosphatidyl Inositol (GPI)-containing lipid ...most GPI-linked proteins, the processing of which results ...
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Expression of a glycosylphosphatidylinositol-anchored ligand, growth hormone, blocks receptor signalling
... In some cell types, autocrine actions of GH are more potent than exogenously administered GH [17–19]. Moreover, autocrine GH producing cells become unresponsive to externally admin- istered GH [20,21]. Both GH–GPI ...
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Distinct Requirements for Tail Anchored Membrane Protein Biogenesis in Escherichia coli
... for membrane targeting and insertion than regular membrane proteins with more TMDs and/or large periplasmic loops that have to be translocated across the inner ...
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Lipid rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions
... the membrane and modify lipids directly with the consequence of profound effects on lipid sig- nalling, organization and dynamics ...of membrane components and an aggregation in defined ...for ...
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Mechanisms of Regulation and Fidelity in Tail-Anchored Membrane Protein Targeting
... Intriguingly, Get3 is catalytically activated through tetramerization (Fig. 2.1, steps 5, 6). This phenomenon was previously suggested by the structure of an MjGet3 tetramer and by the formation of tetrameric Get3/TA ...
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PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis
... soluble form (Additional file 3: Figure S3c), predicted by natural selection analysis and then evaluated by flow cytometry to quantify the protein-cell interaction. The percentage of each recombinant binding to ...
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