gradient gel electrophoresis
Denaturing Gradient Gel Electrophoresis (DGGE)
... Denaturing gradient gel electrophoresis (DGGE) is a molecular fingerprinting method that separates polymerase chain reaction (PCR)-generated DNA ...agarose gel electrophoresis results ...
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Denaturing gradient gel electrophoresis analysis of archaeal and bacterial populations in a submerged anaerobic membrane bioreactor treating landfill leachate at low temperatures
... This study investigated the evolution of archaeal and bacterial populations of two Submerged Anaerobic Membrane Bioreactors (SAMBRs) operating at a mean Solids Residence Time (SRT) of 30 (SAMBR30) and 300 days (SAMBR300) ...
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Temperature Gradient Gel Electrophoresis as a Valuable Accessory Tool for Assessment of Dysbiosis in Crohn’s Disease
... or electrophoresis in denaturing gels of 16SrDNA ...temperature gradient gel electrophoresis (TGGE) and next generation sequencing of 16SV6-V8rDNA amplicons present in gram negative cultures ...
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Single base variation in DNA fragment joined to a GC-clamp can be detected by temporal temperature gradient gel electrophoresis
... temperature gradient gel electrophoresis was first introduced by Yoshino, in ...polyacrylamide gel containing a constant concentration of urea. During electrophoresis, temperature is ...
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High Prevalence of Helicobacter Species Detected in Laboratory Mouse Strains by Multiplex PCR Denaturing Gradient Gel Electrophoresis and Pyrosequencing
... Rodent models have been developed to study the pathogenesis of diseases caused by Helicobacter pylori, as well as by other gastric and intestinal Helicobacter spp., but some murine enteric Helicobacter spp. cause ...
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Denaturing Gradient Gel Electrophoresis of PCR Amplified gki Genes: a New Technique for Tracking Streptococci
... Viridans group streptococci (VGS) are a well-known cause of infections in immunocompromised patients, accounting for severe morbidity and mortality. Streptococcus mitis group species (Streptococcus mitis, Strepto- coccus ...
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Diversity of Hepatitis C Virus Quasispecies Evaluated by Denaturing Gradient Gel Electrophoresis
... Denaturing gradient gel electrophoresis (DGGE) was used to study the diversity of hepatitis C virus (HCV) quasispecies. Optimized DGGE running conditions were applied to screen for variations in ...
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Evaluation of Denaturing Gradient Gel Electrophoresis (DGGE) for the Determination of Marine Phytoplankton Genetic Diversity
... One approach that is gaining popularity in studies on the diversity of bacteria and archaea natural communities is gradient gel electrophoresis (GGE), using either a chemical denaturant ...
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Diversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis
... PCR-denaturing gradient gel electrophoresis (DGGE) fingerprinting was first used for profiling complex microbial populations by Muyzer et al. (1993). It has been widely used in environmental ...
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Rapid Discrimination of Listeria monocytogenes Strains by Microtemperature Gradient Gel Electrophoresis
... Microtemperature gradient gel electrophoresis ( -TGGE) was examined for use for the rapid subtyping of Listeria monocytogenes ...total electrophoresis time of only 7 ...
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Identification of the multiple beta thalassemia mutations by denaturing gradient gel electrophoresis
... denaturing gradient gel electrophoresis to detect the beta-thalassemia mutations in the Chinese ...denaturing gradient gel electrophoresis offers a nonradioactive means of ...
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Search for Localized Dysbiosis in Crohn's Disease Ulcerations by Temporal Temperature Gradient Gel Electrophoresis of 16S rRNA
... temperature gradient gel electrophoresis (TTGE) to compare the qualitative com- positions of the mucosa-associated microbiotas in ulcerated (U) and nonulcerated (NU) regions of the ilea and colons of ...
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Denaturing Gradient Gel Electrophoresis as a Diagnostic Tool in Periodontal Microbiology
... denaturing gradient gel electrophoresis (DGGE) as a tool for the detection of clinically relevant species and to compare the results of detection by DGGE with those by PCR and ...the gel, and ...
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Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles
... Denaturing gradient gel electrophoresis (DGGE) is a more recent fingerprinting tech- nique by which PCR-amplified DNA fragments are separated according to their sequence information ...polyacrylamide ...
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Use of 16S Ribosomal DNA PCR and Denaturing Gradient Gel Electrophoresis for Analysis of the Microfloras of Healing and Nonhealing Chronic Venous Leg Ulcers
... The bacterial microfloras of 8 healing and 10 nonhealing chronic venous leg ulcers were compared by using a combination of cultural analysis and denaturing gradient gel electrophoresis (DGGE) of ...
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Temperature Gradient Gel Electrophoresis of Fecal 16S rRNA Reveals Active Escherichia coli in the Microbiota of Patients with Ulcerative Colitis
... Previous studies of the endogenous microbiota in patients with ulcerative colitis (UC) have not taken bacterial activity into account, yet bacteria with high transcriptional activity might have a more important ...
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Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR
... amide gel under UV illumination. The gel fragments were washed once in 200 μl of sterile deionized water and kept in 50 μl of sterile water overnight at 4°C for ...extracted gel mix was heated at ...
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Comparison of restriction enzyme analysis and pulsed field gradient gel electrophoresis as typing systems for Candida albicans
... Distinguishable restriction enzyme patterns of chromosomal DNAs were produced by using endonucleases MspI and EcoRI.. Agarose gel electrophoresis produced over 100 fragments, with fragme[r] ...
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Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities
... possible gel-to-gel variations in DGGE, it is better to limit the quantity of samples to a number that can be analyzed on a single gel or a few ...
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Evaluation of denaturing gradient gel electrophoresis (DGGE) as a method of mutation detection
... In total 5 of the 13 exons of the PAH gene were evaluated for the presence of mutations by DGGE in a group of 50 independent affected patients, living in the South-East of England and attending the University College ...
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