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Gram Staining

Techniques for controlling variability in gram staining of obligate anaerobes

Techniques for controlling variability in gram staining of obligate anaerobes

... of gram-positive anaerobes either inconsis- tently, rarely, or never stain gram ...as staining consistently gram pos- itive only at 24 h in PYG (peptone, yeast extract, glucose) broth ...a ...

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Extract Ethyl Acetate Red Fruit (Pandanus Conoideus Lam.) as a Counterstain in Gram Staining Technique

Extract Ethyl Acetate Red Fruit (Pandanus Conoideus Lam.) as a Counterstain in Gram Staining Technique

... for Gram-Gram bacteria approaching 60C (strong purplish red) ...in Gram staining produces several colors that almost resemble basic ...

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Evaluation of semi-quantitative scoring of Gram staining or semi-quantitative culture for the diagnosis of ventilator-associated pneumonia: a retrospective comparison with quantitative culture

Evaluation of semi-quantitative scoring of Gram staining or semi-quantitative culture for the diagnosis of ventilator-associated pneumonia: a retrospective comparison with quantitative culture

... of Gram stain is 91%, suggesting that VAP is unlikely if the Gram stain result is negative ...initial Gram stain results. In contrast, a 3+ or more of Gram stain score indicates the necessity ...

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Diagnosis of Meningococcal Meningitis by Broad Range Bacterial PCR with Cerebrospinal Fluid

Diagnosis of Meningococcal Meningitis by Broad Range Bacterial PCR with Cerebrospinal Fluid

... and Gram staining, as well as with the eventual clinical diagnosis for the ...setting, Gram staining and bacterial culture still remain the cornerstones of ...

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Meningitis in a Chinese adult patient caused by Mycoplasma hominis: a rare infection and literature review

Meningitis in a Chinese adult patient caused by Mycoplasma hominis: a rare infection and literature review

... Mycoplasma hominis, initially described as pleuropne umonia-like organism, is a commensal of the human oral cavity, respiratory tract, and genitourinary tract [1–3]. However, its role in the pathogenesis of infec- tions ...

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Algorithm To Determine Cost Savings of Targeting Antimicrobial Therapy Based on Results of Rapid Diagnostic Testing

Algorithm To Determine Cost Savings of Targeting Antimicrobial Therapy Based on Results of Rapid Diagnostic Testing

... 2. Bartlett, J. G., S. F. Dowell, L. A. Mandell, T. M. File, Jr., D. M. Musher, M. J. Fine, et al. 2000. Practice guidelines for the management of commu- nity-acquired pneumonia in adults. Clin. Infect. Dis. 31:347–382. ...

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Bacterial meningitis in children under 15 years of age in Nepal

Bacterial meningitis in children under 15 years of age in Nepal

... (Gram staining and culture re- sult) of CSF facilitates the choice of antibiotic therapy ...[27]. Gram staining, a mainstay of bacterial men- ingitis diagnosis, is widely available, ...

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Characterization of antimicrobial compounds from Streptomyces isolates

Characterization of antimicrobial compounds from Streptomyces isolates

... showed Gram positive reaction in Gram staining; in microscopic characterization aerial mycelium was found filamentous with long highly branched and non fragmenting, long chain containing 30 spores in ...

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Reassessment of the Role of Rapid Antigen Detection Tests in Diagnosis of Invasive Group A Streptococcal Infections

Reassessment of the Role of Rapid Antigen Detection Tests in Diagnosis of Invasive Group A Streptococcal Infections

... Because Gram staining demonstrated a limited contribution to the diagnosis and because the sensitivity of RADTs was decreased with culture-negative samples, we recom- mend their use as first-line diagnostic ...

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Prevalence of Oropharyngeal Candidiasis among HIV Patients Attending ART Clinic, Infectious Disease Hospital (IDH) Kano - Nigeria

Prevalence of Oropharyngeal Candidiasis among HIV Patients Attending ART Clinic, Infectious Disease Hospital (IDH) Kano - Nigeria

... a gram staining positivity rate of 75% compared to 30% in the present ...Direct gram staining of the specimens along with the clinical signs and symptoms of oral candidiasis can be a valuable ...

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Bacterial Identification From Hydrocarbon Polluted Sources And Amplification Of Their Hydrocarbon Degrading Genes

Bacterial Identification From Hydrocarbon Polluted Sources And Amplification Of Their Hydrocarbon Degrading Genes

... with gram staining and four different species of bacteria were identified as Micrococcus luteus, Streptococcus pyogenes, Bacillus subtilis and Corynebacterium kutsceri from two different sources of ...

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The value of simple microbiological studies for on-site screening of acute neonatal conjunctivitis in Angola

The value of simple microbiological studies for on-site screening of acute neonatal conjunctivitis in Angola

... Findings: Neonatal conjunctival smears (n = 95), maternal data, and perinatal factors were collected. Slides were air-dried and sent to the Microbiology Department of the Hospital Clinico Universitario, Valladolid, ...

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Triple diagnostics for early detection of ambivalent necrotizing fasciitis

Triple diagnostics for early detection of ambivalent necrotizing fasciitis

... pus, non-contracting muscle fibres and a positive “finger test” [24–26]. These macroscopic findings are pathogno- monic and should prompt aggressive surgical debridement (Table 1). However, especially in the early phases ...

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The Study of the Microbes Degraded Polystyrene

The Study of the Microbes Degraded Polystyrene

... By Gram staining, the TM1 and ZM1 are showed both Gram negative bacteria. And in the result of TSI agar test, we can confirm that the TM1 and ZM1 are not the same bacteria strain (Table 1). From TSI ...

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The Use of Extracts from Nigerian Indigenous Plants as Staining Techniques in Bacteriology, Mycology and Histopathology

The Use of Extracts from Nigerian Indigenous Plants as Staining Techniques in Bacteriology, Mycology and Histopathology

... Smears of organisms (S. aureus, E. coli, P. aeruginosa, P. mirabilis, K. Pneumoniae) were made on twenty-five sets of clean grease free slide and heat fixed [10, 11]. Gram staining reagents (crystal violet, ...

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Comparison of three staining methods for the detection of intestinal microspora spp.

Comparison of three staining methods for the detection of intestinal microspora spp.

... chromotrope staining method by using aniline blue as the ...standard staining technique by modifying the staining temperature to ...The staining method with calcofluor white that stain chitin ...

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Characterization of Antimicrobial Activity of Some Indian Spices

Characterization of Antimicrobial Activity of Some Indian Spices

... The Gram staining test confirmed the pathogen to be E.coli. It was also confirmed with EMB agar which shows metallic green sheath colonies. Among the ratios comprising cinnamon and clove showed the highest ...

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Rapid detection of bacterial growth in blood cultures by bioluminescent assay of bacterial ATP

Rapid detection of bacterial growth in blood cultures by bioluminescent assay of bacterial ATP

... Detection time of bacterial growth in blood culture bottles inoculated with 10 CFU of bacteria per ml of blood with subculture, bioluminescent assay, and Gram staining Bacterial strains.[r] ...

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Identification and characterization of potential useful bacteria from marine environment

Identification and characterization of potential useful bacteria from marine environment

... Six bacterial strains were successfully isolated based on distinguishable characters as shape, size and pigment productions. Each of the strains was tested for morphological, biochemical and antibiotic sensitivity. The ...

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Biostimulation of Pseudomonas aeruginosa for the Degradation of Atrazine Contaminated Soil

Biostimulation of Pseudomonas aeruginosa for the Degradation of Atrazine Contaminated Soil

... as gram staining, catalase test, oxidase test, citrate test, indole test, urease test, coagulase test and Mac Conkey agar medium (Buchan and Gibbons,1974), were used for characterizing the ...

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