Green Fluorescent Protein (GFP)
Influence of Insertion of the Last Sense Codon on Expression Efficiency of Green Fluorescent Protein Gene in Escherichia coli
... used green fluorescent protein (GFP) gene for our ...study. GFP was dis- covered as a companion protein to aequorin in 1962, the famous chemiluminescent protein from ...
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Influence of Some Point Mutations of Green Fluorescent Protein Gene on Expression Efficiency in <i>Escherichia coli</i> - Volume 7 Number 4 (Aug. 2016) - IJCEA
... Green fluorescent protein (GFP) was discovered as a companion protein to aequorin, the famous chemiluminescent protein from Aequorea jellyfish ...[1]. GFP is composed of ...
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The Role of Green Fluorescent Protein (GFP) in Transgenic Plants to Reduce Gene Silencing Phenomena
... The green fluorescent protein (GFP) of jellyfish (Aequorea victoria) has significant advantages over other reporter genes, because expression can be detected in living cells without any ...
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Transformation of Epichloë typhina by electroporation of conidia
... ardgrass. Green Fluorescent Protein (GFP) has been uti- lized extensively as a marker to aid in the development of fungal transformation systems and to examine early stages in plant/fungal ...
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Inteins—A Focus on the Biotechnological Applications of Splicing Promoting Proteins
... the green fluorescent protein (GFP) [48], and a modified method is also available using the DnaE split intein from Nostoc puntiforme [49] [50], facilitating an ultra-rapid and cost efficient ...
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A tracer-based method enables tracking of Plasmodium falciparum malaria parasites during human skin infection
... as green fluorescent protein (GFP) or luciferase provided unprecedented insight into host-to-host parasite transmission and subsequent migratory behaviour of sporozoites from skin to ...
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Papillomavirus Capsid Protein Expression Level Depends on the Match between Codon Usage and tRNA Availability
... the green fluorescent protein (gfp) gene with those frequently used in PV late genes did not alter gfp transcription in Cos-1 cells but almost abolished translation, supporting the ...
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Construction of Various γ34.5 Deleted Fluorescent-Expressing Oncolytic herpes Simplex type 1 (oHSV) for Generation and Isolation of HSV-Based Vectors
... Background: Oncolytic herpes simplex virus (oHSV)-based vectors lacking γ34.5 gene, are considered as ideal templates to construct efficient vectors for (targeted) cancer gene therapy. Herein, we reported the ...
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Original Article Depleting ABCE1 expression induces apoptosis and inhibits the ability of proliferation and migration of human esophageal carcinoma cells
... target protein-RNase L, were first studied in tumor tissues of esophageal carcinoma and adjacent non-tumor ...siRNA green fluorescent protein (GFP) expression vector of ABCE1 was ...
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Spatial and temporal localisation of exopolysaccharide gene expression in mucoid and non mucoid Pseudomonas aeruginosa biofilms : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Microbiology, Massey University, Manawatu, New Zealand
... To observe the spatial localisation of cells expressing each EPS gene, this strain will be transformed with a plasmid encoding the expression of the stable green fluorescent protein gfp [r] ...
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Use of Single-Cycle Infectious Lymphocytic Choriomeningitis Virus To Study Hemorrhagic Fever Arenaviruses
... the green fluorescent protein ...the GFP-based microneutralization assay we developed was similar to that obtained with a conventionally used focus reduction neutralization (FRNT) ...
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In vitro quantitative analysis of Salmonella typhimurium preference for amino acids secreted by human breast tumor
... Bacterial therapies have been paid significant attentions by their ability to penetrate deep into the solid tumor tis‑ sue and its propensity to naturally accumulate in tumors of living animals. Understanding the actual ...
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Specialized mouse embryonic stem cells for studying vascular development
... express green fluorescent protein (GFP) under the endothelial promoter, Tie-2, and Discomsoma ...red fluorescent protein (RFP) under the promoter for alpha-smooth muscle actin ...
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The HIV-1-Specific Protein Casp8p41 Induces Death of Infected Cells through Bax/Bak
... novel protein generated when HIV-1 protease cleaves caspase 8, independently causes NF- B activation, proinflammatory cytokine production, and cell ...a green fluorescent protein ...
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Hyperphosphorylation of the Rotavirus NSP5 Protein Is Independent of Serine 67 or NSP2, and the Intrinsic Insolubility of NSP5 Is Regulated by Cellular Phosphatases
... to green fluorescent protein (GFP) conferred both insolubility and GFP localization into viroplasm-like structures, in the ab- sence of hyperphosphorylation, indicating that the NSP5 C ...
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Induction of a robust immune response against avian influenza virus following transdermal inoculation with H5-DNA vaccine formulated in modified dendrimer-based delivery system in mouse model
... pBud-H5-green fluorescent protein (GFP)-interferon-regulatory factor (IRF)3 following delivery using polyamidoamine (PAMAM) dendrimer and transactiva- tor of transcription (TAT)-conjugated ...
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Generation of bovine transgenics using somatic cell nuclear transfer
... milk protein concentration ...the green fluorescent protein (GFP) gene transfected donor cells and subsequently used for NT to create bovine embryos and ...calves. Green ...
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High frequency, cell type-specific visualization of fluorescent-tagged genomic sites in interphase and mitotic cells of living Arabidopsis plants
... red fluorescent protein; EGFP or just G: enhanced green fluorescent protein; GFP: green fluorescent protein; R: DsRed2; mRed or mR: DsRed-monomer ...
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Intercellular Trafficking of VP22, a Herpes Simplex Virus Type 1 Tegument Protein
... the protein through dissolving the cellular membrane causes an artifact that confuses the results and leads to misinterpretation as intracellular translocation of the protein ...trafficking, Green ...
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A Visual Screen of Protein Localization during Sporulation Identifies New Components of Prospore Membrane-Associated Complexes in Budding Yeast
... membranes. Green fluorescent protein (GFP) fusions to various proteins that associate with septins at the bud neck during vegetative growth as well as to proteins encoded by genes that are ...
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