Gus, Gus
Engineering the glycoside hydrolase β-glucuronidase (β-GUS) to improve a non-native activity for the synthesis of O-glucuronides
... Other observations that had been pointed out in the mutational tolerance studies may also facilitate future enzyme engineering endeavours of the -GUS enzyme. When we performed site saturation mutagenesis on the ...
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Impact of ubiquitous inhibitors on the GUS gene reporter system: evidence from the model plants Arabidopsis, tobacco and rice and correction methods for quantitative assays of transgenic and endogenous GUS
... (B) GUS in Arabidopsis leaves as measured experimentally and cor- rected for the estimated inhibitory capacity of the ...endogenous GUS activity have been measured at different extract concentrations: ...
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Transformation and confirmation of GUS gene expression in Solanum melongena L. of PLR 1 cultivar
... study GUS gene transformation was carried out in eggplant using Agrobacterium strain with pBAL2 vector harboring gus gene and nptII as selection marker ...transient gus gene expression are different ...
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Innovation activities in Polish industry in 1998-2000: main results from the GUS 2001 innovation survey
... The paper analysed results of GUS innovation survey in manufacturing for 1998-2000 and innovation survey in services for 1997-1999. Innovation activities in 1998-2000 reflect downturn in Polish economy of this ...
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Kouznetsov, Laura (2008): Wissen über HIV/AIDS und ihre Prävention bei Aussiedlern und jüdischen Kontingentflüchtlingen aus der Gemeinschaft Unabhängiger Staaten (GUS) in Bayern. Dissertation, LMU München: Medizinische Fakultät
... Die GUS-Immigranten waren sehr gut über HIV-Übertragungsrisiko durch kontaminierte Spritzen/Nadeln, Vaginalverkehr und über die vertikale HIV-Übertragung ...
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Characterization of a Novel RING Type Ubiquitin E3 Ligase GhRING2 Differentially Expressed in Cotton Fiber
... The ubiquitin-proteasome proteolysis pathway is responsible for the degradation of abnormal and short-lived proteins to regulate many important biochemical activities in eukaryotes. By em- ploying affymetrix microarray ...
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Suppression of long-distance movement of tobacco etch virus in a nonsusceptible host.
... The rates of TEV-GUS infection focus expansion, which reflect the rate of cell-to-cell movement, were similar in inoc- ulated leaves of V20 and Havana425, although the timing of focus expansion was delayed by ...
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The roles of Arabidopsis HSFA2, HSFA4a, and HSFA7a in the heat shock response and cytosolic protein response
... In addition to heat shock (HS), a proline analog, aze- tidine-2-carboxylic acid (AZC), can induce accumulation of abnormal-misfolded proteins in the cytosol to trigger the CPR by modulating HSFA2 activity (Yeh et al. ...
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Comparison of Beta Glucuronidase Expression and Anatomical Localization in Bombarded Immature Embryos of Banana Cultivar Mas via Biolistic Transformation
... high GUS activity was always achieved at 6 and 9 cm target distance with 900, 1100 and 1350 psi helium pressure ...the GUS activity for the putative transformed immature embryos revealed no significant (P ...
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In the absence of BYPASS1 related gene function, the bps signal disrupts embryogenesis by an auxin independent mechanism
... Analysis of bps triple mutant embryos revealed changes in expression of both auxin-related markers (PIN1 and DR5) and developmentally important transcription factors (PLT1, PLT2 and WOX5). The growth, patterning and cell ...
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Expression of the Arabidopsis Transposable Element Tag1 Is Targeted to Developing Gametophytes
... The Arabidopsis transposon Tag1 undergoes late excision during vegetative and germinal development in plants containing 35S-Tag1-GUS constructs. To determine if transcriptional regulation can account for the ...
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PubMedCentral-PMC4889055.pdf
... Having identified intron 1 as important for the sucrose response of PAP1, we next used PLACE to scan intron 1 for known cis-elements and identified the SURE-1 sucrose response element (TTTTCTATT) (Fig 3A). Interestingly, ...
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... At the J. Willard Marriott Library at the University of Utah, teaching librarians span across departments and units. However, there has always been a core teaching department within the library that is now titled ...
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Androgen regulation of murine beta-glucuronidase expression: identification and characterization of a nonresponse variant.
... One of the major features of P-glucuronidase (GUS) expression in inbred strains of the house mouse, Mus musculus, is the responsiveness of this enzyme to androgen [r] ...
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Multiple tissue-specific expression of rice seed-shattering gene SH4 regulated by its promoter pSH4
... the GUS reporter gene driven by pSH4 had the highest level (~55%) of expression in the seed-pedicel junction region of mature panicles, relative to that of driven by 35S ...
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Two genetic elements regulate murine beta-glucuronidase synthesis following transcript accumulation.
... T o determine whether Gus-u and Gus-t regulate GUS synthesis by altering levels of GUS transcripts, we assessed the extent of S1 nuclease protection of a GUS antisense [r] ...
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Tissue Culture-independent In Planta Transformation Strategy: an Agrobacterium tumefaciens-Mediated Gene Transfer Method to Overcome Recalcitrance in Cotton (Gossypium hirsutum L.)
... For GUS activity in the pollen, flowers with undehisced pollen were ...to GUS histochemical analysis in an eppendorf tube, the flowers were crushed gently with a glass rod to release the pollen and the ...
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Characterisation and expression of the glutamine synthetase g1n α gene of french bean
... Histochemical localisation of GUS activity in the vascular tissue of a transgenic gIn-a: :uidA tobacco plant leaf 24 hours after mechanical wounding... Histochemical localisation of GUS [r] ...
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In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA
... observed gus expression in the leaves (Figure 1B) and no significant phenotypical differences were observed in the transgenic lines when compared with wild-type ...the GUS-RNAi lines (Figure ...the ...
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Dissection of a Synthesized Quantitative Trait to Characterize Transgene Interactions
... Six transgenic tobacco lines, each homozygous for the P-glucuronidase (GUS) gene at a different locus, and wild type were selfed and intercrossed to evaluate GUS activity [r] ...
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