H+ exchanger
Identification of the human NHE 1 form of Na(+) H+ exchanger in rabbit renal brush border membranes
... Na(+)-H+ exchanger (NHE-1) and the renal brush border membrane (BBM) Na(+)-H+ exchanger, polyclonal antibodies to synthetic peptides representing a putative external (Ab-E) and an internal ...
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Activation of protein kinase A acutely inhibits and phosphorylates Na/H exchanger NHE 3
... exchanger is likely encoded by NHE-3. The present studies examined regulation of NHE-3 by PKA. NHE-3 was stably expressed in Na/H exchanger-deficient fibroblasts (AP-1/NHE-3 cells). PKA activation ...
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Responses of the Na+/H+ exchanger of european flounder red blood cells to hypertonic, β adrenergic and acidotic stimuli
... The β-adrenergic response is likely to be a more recent evolutionary development linked to the development of oxygen secretion mechanisms in the swimbladder and choroid retia and of haemoglobins demonstrating a Root ...
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CAMP mediated inhibition of the renal brush border membrane Na+ H+ exchanger requires a dissociable phosphoprotein cofactor
... Prior studies have suggested that protein kinase A (PKA)-mediated inhibition of the rabbit renal brush border membrane (BBM) Na(+)-H+ exchanger involves a regulatory protein that is distinct from the ...
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Characterization of a protein cofactor that mediates protein kinase A regulation of the renal brush border membrane Na(+) H+ exchanger
... Abstract Activation of cAMP-dependent protein kinase A inhibits the renal proximal tubule brush border membrane Na+-H+ exchanger by a process involving participation of a regulatory cofa[r] ...
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The human Na+/H+ exchanger 1 is a membrane scaffold protein for extracellular signal-regulated kinase 2
... The plasma membrane Na + /H + exchanger 1 (NHE1, SLC9A1) is a major regulator of pH and volume in es- sentially all cells studied. Furthermore, NHE1 is involved in the regulation of cell proliferation, ...
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In vitro susceptibility to quinine and microsatellite variations of the Plasmodium falciparum Na+/H+ exchanger (Pfnhe 1) gene: the absence of association in clinical isolates from the Republic of Congo
... Background: Quinine is still recommended as an effective therapy for severe cases of Plasmodium falciparum malaria, but the parasite has developed resistance to the drug in some cases. Investigations into the genetic ...
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Suppression of breast cancer cell growth by Na+/H+ exchanger regulatory factor 1 (NHERF1)
... cells a Breast cancer cell lines, as indicated, were Knockdown of Na+/H+ exchanger regulatory factor 1 NHERF1 expression in breast cancer cells.. analysed for NHERF1 expression by immuno[r] ...
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Structure function of recombinant Na/H exchanger regulatory factor (NHE RF)
... using solubilized rabbit renal BBM and a reconstitution assay have suggested that the regulatory effect of PKA can be disso- ciated from the basal rate of transport (7–10). These observa- tions resulted in the initial ...
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Restoration of Na+/H+ exchanger NHE3 containing macrocomplexes ameliorates diabetes associated fluid loss
... We found that the expression of Na+/H+ exchanger NHE3 and several scaffold proteins, including NHE3 regulatory factors NHERFs, inositol trisphosphate IP3 receptor-binding protein release[r] ...
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Analysis of Plasmodium falciparum Na+/H+ exchanger (pfnhe1) polymorphisms among imported African malaria parasites isolated in Wuhan, Central China
... DNA was amplified by nested PCR (Bio-Rad Mini MJ thermal cycler). The primary primers including NHE-A and NHE-B, and the secondary primers containing NHE-C and NHE-D were described in a previous study [29 – 31]. No ...
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Elevated Na/H exchanger 1 (SLC9A1) emerges as a marker for tumorigenesis and prognosis in gliomas
... Fixed brain sections (4% PFA) were mounted on micro- scope slides. Sections were then incubated with a block- ing solution (0.3% Triton X-100 and 3% goat serum in PBS) for 60 min at room temperature (RT) and probed with ...
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NHEVNAT: an H+ V ATPase electrically coupled to a Na+:nutrient amino acid transporter (NAT) forms an Na+/H+ exchanger (NHE)
... The preceding discussion has been based on data published in refereed journals. Here, we speculate on the implications of the data for ionic homeostasis. To make the case that AgNHA1 is a candidate for the recycling ...
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Basolateral Na+/HCO3– cotransport activity is regulated by the dissociable Na+/H+ exchanger regulatory factor
... To determine the specific role of NHE-RF in the regulation of basolateral NBC activity, the effect of recombinant NHE-RF on NBC activity was examined using solubilized rabbit renal BLM p[r] ...
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Glucocorticoids enhance acid activation of the Na+/H+ exchanger 3 (NHE3)
... 1 h, blots were probed in the same buffer for 1 h with a polyclonal anti–opossum NHE3 antibody (antiserum 5683, generated against a maltose binding protein/NHE3 [aa 484–839] fusion protein) at a dilution of ...
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Compensatory regulation of Na+ absorption by Na+/H+ exchanger and Na+-Cl- cotransporter in zebrafish (Danio rerio)
... Compensatory regulation of NHE3b and NCC in zebrafish Na + homeostasis appears to be of physiological significance, based on analyses of these transport pathways during acclimation to environmental stress, such as acidic ...
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Regulation of the rabbit ileal brush border Na+/H+ exchanger by an ATP requiring Ca++/calmodulin mediated process
... thawing vesicles with 0.85 microM free Ca++ plus 5 microM exogenous CaM failed to alter Na+/H+ exchange as did the addition of exogenous ATP plus an ATP regenerating system, which was sufficient to elevate ...
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The Seasonal Modulation of Na+/H+ Exchanger Activity in Trout Erythrocytes
... The time course of recovery of the maximal isoproterenol-stimulated efflux of H + equivalents in trout erythrocytes following bleeding and washing of the cells into a buffered trout sali[r] ...
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A vacuolar type H+ ATPase and a Na+/H+ exchanger contribute to intracellular pH regulation in cockroach salivary ducts
... of H + secretion (Schwartz and Al-Awqati, 1985; Chambrey et ...stimulated H + secretion via V-ATPase in kidney proximal tubule cells occurs by a protein kinase A-independent mechanism but that protein ...
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The temperature dependence of the adrenergic Na+/H+ exchanger of trout erythrocytes
... The effects of cooling upon the steady states that follow adrenergic stimulation are quite clear: a progressive reduction in the resulting changes in both pHi and [Na+]j and a considerab[r] ...
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