H2A-H2B
Transcription-coupled eviction of histones H2A/H2B governs V(D)J recombination.
... cutting. H2A/H2B eviction occurs with a displacement time of approximately six minutes in vivo (Kimura and Cook, 2001); this fits well with our observed transient accessibility of the ...
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Hondele, Maria (2013): An integrated view of the essential eukaryotic chaperone FACT in complex with histones H2A-H2B. Dissertation, LMU München: Medizinische Fakultät
... histone H2A, switches almost 1808 in orientation and is captured into an antiparallel b-sheet with the edge b-strand of the concave chaperone b-sand- wich, thus extending the b-strand ...the H2A–H2B ...
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Depletion of H2A-H2B Dimers in Saccharomyces cerevisiae Triggers Meiotic Arrest by Reducing IME1 Expression and Activating the BUB2-Dependen Branch of the Spindle Checkpoint
... histone H2A-H2B dimers at a lower level than do wild-type ...the H2A-H2B dimer blocks sporulation by at least two mechanisms: disruption of the expression of meiotic regulatory genes and ...
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Autoantibodies associated with lupus induced by diverse drugs target a similar epitope in the (H2A H2B) DNA complex
... the H2A-H2B dimer or that the complete epitope is generated by a surface feature involving H2A-H2B and ...anti-[(H2A- H2B)-DNA] reactivity in six of six sera from patients with ...
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Genetic dissection of SLE pathogenesis Sle1 on murine chromosome 1 leads to a selective loss of tolerance to H2A/H2B/DNA subnucleosomes
... (A) Splenic T cells from 3–7-mo-old B6 mice (open dots, n 5 9) or B6.NZMc1 mice (closed dots, n 5 11) were cocultured with irradi- ated, syngeneic splenic T-depleted APCs, with 1 m g/ml of histones H2A, ...
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A Simple Method to Produce Sub Nucleosome Complexes of High Purity In Vitro
... With the identification of increasing number of chromatin modifiers, histone variants, histone post-translational modifications and their cross-talk, it is essential to validate these findings and interactions in vitro ...
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Construction of Comprehensive Dosage-Matching Core Histone Mutant Libraries for Saccharomyces cerevisiae
... synthetic H2A/H2B genes were compared in rich ...histone H2A also did not show significant differences among strains, both in protein and mRNA level (Figure 2D and Figure S2 in File ...
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Distinctive patterns of epigenetic marks are associated with promoter regions of mouse LINE-1 and LTR retrotransposons
... The fundamental unit of chromatin is an array of tan- dem nucleosomes each composed of two copies of the core histones H2A, H2B, H3 and H4 wrapped around genomic DNA. Changes in histone composition and ...
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Chromatin-like structures obtained after alkaline disruption of bovine and human papillomaviruses.
... Complexes of viral DNA and present in the agarose column fractions, and low-molecular-weight polypeptides correspond- histone preparations 1.8 mg/ml in 0.2 M acetic ing to H2a, H2b, H3, [r] ...
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The SONBNUP98 Nucleoporin Interacts With the NIMA Kinase in Aspergillus nidulans
... mented strains by genomic DNA preparation and Escherichia gesting a role in S-phase progression. We isolated the coli transformation were retested for their ability to comple- histone H2A-H2B gene pair as a ...
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Linking Entrepreneurial Orientation to Firm Performance in a Post-Socialist Market Context: the Case of Hungary
... However, this conceptual model might be too simple, and would be easily obtainable by organizations, thus the argument of Lumpkin and Dess (1996) is taken into account. They argue that the impact of EO on performance is ...
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Residues in the Nucleosome Acidic Patch Regulate Histone Occupancy and Are Important for FACT Binding in Saccharomyces cerevisiae
... residues, H2B L109 and H2A E57, are important for binding of H2A-H2B dimers to the FACT histone chaperone in ...of H2B M62 and Y45, as has been previously reported (Kemble et ...an ...
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PubMedCentral-PMC4919428.pdf
... to as prey), such as TAP and FLAG purifications. In our analy- sis, the specific bait purifications (Spt16-TAP LS) are compared with mock purifications from untagged parental strains. Using SAINT, we analyzed the four ...
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Progression Into the First Meiotic Division Is Sensitive to Histone HN-HZB Dimer Concentration in Saccharomyces cerevisiae
... are similar in the mitotic and meiotic cell cycles: The second model hypothesized that the two H2A-H2B gene pairs are expressed differently in vegetative and sporu- lating [r] ...
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Facteurs impliqués dans le remodelage de la chromatine au cours de la spermiogenèse
... Ainsi, chez la souris et chez l'homme, les histones coeur du nuclEosome H2A, H2B, H3 et H4 sont acEtylEes dans les spermatogonies et les spermatocytes au stade prEleptot~ne, [r] ...
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Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study
... The octamer-shaped nucleohistone complex consists of histones H2A, H2B, histone 3 (H3) and histone (H4), all involved in sperm DNA packaging. Along with prot- amines, they form a tightly coiled and ...
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Genetic Analysis of Saccharomyces cerevisiae H2A Serine 129 Mutant Suggests a Functional Relationship Between H2A and the Sister-Chromatid Cohesion Partners Csm3–Tof1 for the Repair of Topoisomerase I-Induced DNA Damage
... Csm3, Mrc1, and Tof1 were originally identified as checkpoint proteins involved in transmitting the DNA replication arrest to downstream effectors (A lcasabas et al. 2001; F oss 2001; T anaka and R ussell 2001; T ong et ...
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Comprehensive analysis of histone post-translational modifications in mouse and human male germ cells
... male germ cells using antibody-based approaches, and recent studies have utilized mass spectrometry to iden- tify histone PTMs in mature mouse sperm [25]. However, to date, a comprehensive ‘map’ and detailed investiga- ...
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Recent knowledge concerning mammalian sperm chromatin organization and its potential weaknesses when facing oxidative challenge
... Among histone PTM occurring during spermiogene- sis, simultaneous hyperacetylation and ubiquitinylation seem to play an important role in the histone-PRM ex- change. H2A and H2B ubiquitinylation adds a ...
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"Cullin 4 makes its mark on chromatin"
... ubiquitylated H2A [18] when cells sense the genotoxic stress [36,37] (Figure 2A, these responses could be detected at 0 min after UV ...of H2A [18] (Figure ...histone H2A by the Ring2 or Cul4 his- ...
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