H9c2 cardiomyocytes
<p>Dexmedetomidine protects H9c2 cardiomyocytes against oxygen-glucose deprivation/reoxygenation-induced intracellular calcium overload and apoptosis through regulating FKBP12.6/RyR2 signaling</p>
... effects of DEX, as many studies showed that SERCA played an important role on the regulation of Ca 2+ homeostasis in myocardial I/R injury. 53–55 Third, the current results in H9c2 cardiomyocytes may not be ...
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‘Yang Invigorating’ Chinese Tonic Herbs Enhance Mitochondrial ATP Generation in H9c2 Cardiomyocytes
... Figures 1. Effects of Yang and Yin Chinese tonic herbs on mitochondrial ATP generation capacity in H9c2 cardiomyocytes. (a) ‘Yang-invigorating’; (b)‘Yin-nourishing’. (Cells were treated with herbal extracts ...
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Modulation of NOX4 and MAPK Signaling Pathways by Parkia speciosa Empty Pods in H9c2 Cardiomyocytes Exposed to H2O2
... H9c2 cardiomyocytes (ATCC, Manassas, Virginia, USA) were grown in Dulbecco's modified Eagle medium (DMEM) containing 1 % penicillin/streptomycin/ amphotericin B solution (Gibco, Grand Island, NY, USA) and ...
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“Qi Invigorating” Chinese Tonic Herbs (Shens) Stimulate Mitochondrial ATP Generation Capacity in H9c2 Cardiomyocytes in Situ and Rat Hearts ex Vivo
... extracts of all three types of “Shens” (namely, Renshen, Xiyangshen and Dangshen) increased mitochondrial ATP-GC in H9c2 cardiomyocytes, with Renshen being most potent among the tested “Shens”. This ...
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Original Article Acacetin protective myocardial ischemia/reperfusion injury and inhibits apoptosis of H9c2 cardiomyocytes via the PI3K-Akt pathway
... Abstract: Acacetin (5,7-dihydroxy-4’-methoxyflavone) a natural flavonoid compound extracted from natural plants, exhibits a wide range of biological activities, including antioxidant and anti-myocardial ...
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Original Article Panax notoginseng saponins protects H9c2 cardiomyocytes against hypoxia/reoxygenation injury via activating the JAK2/STAT3 pathway
... Janus kinase-signal transducer and activator of transcription (JAK/STAT) pathway is a stress- responsive mechanism that has been implicat- ed in variety of cardiac pathophysiologic pro- cesses including hypertrophy, ...
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Original Article Long noncoding RNA SRA1 attenuates hypoxia-induced injury in H9c2 cardiomyocytes through regulating PPARγ/NF-κB signaling pathway
... Accumulating studies have reported the key role of lncRNAs in heart failure. Yu et al. found that plasma lncRNA UCA 1 could be used as a promising biomarker to diagnose and predict poor outcomes of chronic heart failure ...
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Inhibition of gap junction intercellular communication is involved in silica nanoparticles-induced H9c2 cardiomyocytes apoptosis via the mitochondrial pathway
... in cardiomyocytes suggests that GJIC transmits particular survival signals or inhibits death signals that are activated when cells are exposed to ...in H9c2 cells are probably first or second messengers, ...
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Original Article Paeonol protects H9C2 cardiomyocytes from ischemia/reperfusion injury by activating Notch1 signaling pathway in vitro
... ment was to explore the role of the Notch1 sig- naling pathway in paeonol’s protective effect on SIR-treated H9c2 cells. We determined whether treatment with DAPT, a specific inhibi- tor of the Notch1 signaling ...
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Original Article Ad-HGF induces autophagy in hypoxia-injured H9c2 cardiomyocytes via activating NF-κB signaling
... Supplementary Figure 1. Choose the appropriate multiplicity of infection (MOI) of Ad-HGF to infect the H9c2 cells. A: Different MOIs of Ad-HGF (1000, 100, 10, 1, 0.1 MOI) were used to infect the H9c2 cells ...
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Original Article MicroRNA-182-5p protects H9c2 cardiomyocytes from hypoxia-induced apoptosis by down-regulation of PTEN
... Annexin V-FITC/PI apoptosis detection kit (Beijing Biosea Biotechnology, Beijing, China). The H9c2 cells (100,000 cells/well) were seed- ed in 6 well-plates. Treated cells were washed twice with cold PBS and ...
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The dual topoisomerase inhibitor A35 preferentially and specially targets topoisomerase 2 aby enhancing pre-strand and post-strand cleavage and inhibiting DNA religation
... with cardiomyocytes and animal hearts has not been ...rat H9C2 cardiomyocytes and nude mice, and we demonstrated that A35 did not induce DNA breakage, mitochondrial injury, apoptosis and p53-mediated ...
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Deletion of Osteopontin Enhances b2-Adrenergic Receptor-Dependent Anti-Fibrotic Signaling in Cardiomyocytes
... signaling, including 2 AR-induced cAMP production, in H9c2 cardiomyocytes. Epac1 upregulation by OPN CRISPR-mediated deletion in H9c2 cardiomyocytes 158[r] ...
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Transcription factor MITF regulates cardiac growth and hypertrophy
... of H9C2 cardiomyocytes, MC-9 mast cells, B16 melanoma cells, rat basophilic leukemia (RBL) cells, and primary culture of rat neonatal cardiomyocytes ...in cardiomyocytes at approximately the ...
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Antioxidative action of N a tosyl L lysine chloromethyl ketone prevents death of glutathione depleted cardiomyocytes induced by hydrogen peroxide
... protect the cells from cell damages. The other serine protease inhibitors that were tested could not prevent the cell damages in GSH- depleted H9c2 cells. Pretreatment with an in- hibitor of nuclear factor- ...
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Original Article MiR-26a enhances hypoxia-mediated cardiomyocytes apoptosis by targeting CDK8
... protecting cardiomyocytes from hypoxia-induced apoptosis are ...of cardiomyocytes and provide more information for the development of new treatments of ...neonatal cardiomyocytes by targeting CDK8. ...
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Aspalathin Protects the Heart against Hyperglycemia-Induced Oxidative Damage by Up-regulating Nrf2 Expression
... protects H9c2 cardiomyocytes against high glucose (HG)-induced oxidative damage by improving myocardial substrate metabolism via AMPK regulation ...in cardiomyocytes, the control of ...
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3,3′-Diindolylmethane protects cardiomyocytes from LPS-induced inflammatory response and apoptosis
... Methods: H9C2 cells were incubated with DIM (10, 20 and 30 μ M) with or without LPS for 24 ...in H9C2 cardiomyocytes according to the results detected by flow ...
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Original Article Inhibition of mTORC1 renders cardiac protection against lipopolysaccharide
... Heart lysates of mice and H9c2 cells lysates were prepared in lysis buffer (20 mM Tris, 150 mM NaCl, 10% glycerol, 20 mM glycerophos- phate, 1% NP40, 5 mM EDTA, 0.5 mM EGTA, 1 mM Na3VO4, 0.5 mM PMSF, 1 mM benzami- ...
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Original Article Interleukin-33 prevents high glucose-induced apoptosis in H9c2 cardiac cells by inhibiting reaction oxygen species- activated JNK and p38 MAPK pathway
... There is clear evidence linking increased oxida- tive stress, in particular ROS production, to the development of diabetic cardiovascular compli- cations [26]. In diabetes, alteration of energy substrates can directly or ...
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