Hairpin RNAs
Targeted Gene Silencing in the Model Mushroom Coprinopsis cinerea (Coprinus cinereus) by Expression of Homologous Hairpin RNAs
... homologous hairpin RNA. Such hairpin constructs were shown to be effective in gene silencing in animals, plants, and other fungi (see the introduction for ref- erences), including the dimorphic ...
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Effective Suppression of Human Immunodeficiency Virus Type 1 through a Combination of Short- or Long-Hairpin RNAs Targeting Essential Sequences for Retroviral Integration
... short- hairpin RNAs (shRNAs) has been advanced by the develop- ment of vector systems for efficient delivery and stable expres- sion of shRNA sequences (4, 7, 22, ...double-stranded RNAs, termed ...
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In vitro inhibition of transmissible gastroenteritis coronavirus replication in swine testicular cells by short hairpin RNAs targeting the ORF 7 gene
... short hairpin RNAs (shRNAs), is capable of inhibiting virus replication and protecting swine testicular (ST) cells from the destruction induced by TGEV, which may be not only a new anti-TGEV strategy, but ...
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Small Hairpin RNAs and the Origin of Protein Synthesis
... A model of the early RNA world is proposed. Nearly self-complementary sequences that could adopt double-stranded, small hairpin-like (shRNA), structures would be selected for due to their greater hydrolytic ...
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Inhibition of Hepatitis C Virus in Chimeric Mice by Short Synthetic Hairpin RNAs: Sequence Analysis of Surviving Virus Shows Added Selective Pressure of Combination Therapy
... tients infected with HCV genotype 1. It has been reported that the human hepatocytes in the uPA-SCID chimeric mouse model have the capacity to produce HCV virions at a rate comparable to that of the hepatocytes in ...
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Targeting of human interleukin-12B by small hairpin RNAs in xenografted psoriatic skin
... As evident from Figure 5b, treatment with shIL12B- encoding lentiviral vectors did not seem to have an effect on the clinical phenotype of the psoriatic skin compared to the negative con[r] ...
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Generation of shrnas from randomized oligonucleotides
... The majority of vector-based siRNAs are generated from short hairpin RNAs (shRNAs). In this system, a single Pol III promoter (e.g., H1 or U6) drives transcription of a single species of RNA molecule (8, 9) ...
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PDGFA/PDGFRα-regulated GOLM1 promotes human glioma progression through activation of AKT
... Glioma cell lines were modified with lentiviral constructs expressing short hairpin RNAs targeting GOLM1 or overexpressing the protein to assess function in proliferation, viability, and[r] ...
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Therapeutic benefit derived from RNAi mediated ablation of IMPDH1 transcripts in a murine model of autosomal dominant retinitis pigmentosa (RP10)
... short hairpin RNAs (shRNA) validated in vitro and in vivo, targeting both human and mouse IMPDH1, substantially suppresses the negative pathological effects of mutant IMPDH1, at a point where, in the ...
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A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis
... short hairpin RNAs (shRNAs) against human vascular endothelial growth factor receptor 2 (VEGFR2), chemokine C-C motif receptor 1 (CCR1), and epithelial cell adhesion molecule (EpCAM), three genes closely ...
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AXL modulates extracellular matrix protein expression and is essential for invasion and metastasis in endometrial cancer
... To examine the role of AXL in proliferation of EC cells, we used short hairpin RNAs shRNAs to generate AXL-deficient metastatic endometrial cancer cell lines ARK 1 and Hec50a as describe[r] ...
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Characterization of Viral and Human RNAs Smaller than Canonical MicroRNAs
... small RNAs, such as microRNAs (miRNAs), across a wide range of organisms ...small RNAs on an unprecedented scale across distantly related species, including animals, fungi, unicellular al- gae, plants, and ...
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The 3′ Untranslated Region of Pea Enation Mosaic Virus Contains Two T-Shaped, Ribosome-Binding, Cap-Independent Translation Enhancers
... Termination of translation in eukaryotes occurs when a translat- ing ribosome encounters a stop codon in the A-site. A ternary complex containing release factors and GTP binds to the vacant A-site through tRNA mimicry, ...
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Small RNAs in early mammalian development: from gametes to gastrulation
... non-coding RNAs. These RNAs range in size from 18 to 32 nucleotides (nt) in length and have emerged in the past decades as major players in post- transcriptional regulation across many, if not most, ...
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Spurious Response Suppression in Hairpin Filter Using CSRR Merged in the Filter Structure
... Recent progresses in modern ultra wide-band radar systems and wireless communications demand high-performance and reconfigurable RF subsystems including filters [1]. Parallel coupled line microstrip filters (PCMFs), ...
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Long noncoding RNAs coordinate functions between mitochondria and the nucleus
... Mitochondria have distinct genetic systems that need to be coordinated with the nuclear genetic system, to meet the cellular energy and metabolite needs [5]. In the coordinated signaling system, many proteins encoded by ...
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Stabilization of TM Trimer Interactions during Activation of Moloney Murine Leukemia Virus Env
... The transmembrane subunit (TM) of the trimeric retrovirus Env complex is thought to direct virus-cell membrane fusion by refolding into a cell membrane-interacting, extended form that subsequently folds back on itself ...
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Databases and resources for human small non-coding RNAs
... non-coding RNAs (sRNAs), which were named micro-RNAs ...coding RNAs that have emerged as key players in post-transcriptional gene ...interfering RNAs (endo-siRNAs) 5 and piwi-interacting ...
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In silico identification and target prediction of micrornas in Sesame (sesamum indicum L.) expressed sequence tags
... ABSTRACT. Sesame (Sesamum indicum L.), a member of the Pedaliaceae family, is one of the oldest oilseed crops. For its high oil content, it is known as the “queen of oilseeds”. MicroRNAs (miRNAs) represent a class of ...
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On hairpin vortices in a transitional boundary layer
... In a turbulent spot Fig. 4 typical configurations of negative vorticity on the top, negative vorticity on the bottom and low velocity region in between could be recognized forming hairpin vortices packets oriented ...
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