HCV NS5A domain I
Suppression of Viral RNA Binding and the Assembly of Infectious Hepatitis C Virus Particles In Vitro by Cyclophilin Inhibitors
... of NS5A to- ward RNA replication and virion assembly are ...mains I and II are likely to be required for replication, domain III is essential for efficient production of infectious particles but dis- ...
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Hepatitis C Virus Infection Suppresses GLUT2 Gene Expression via Downregulation of Hepatocyte Nuclear Factor 1α
... how HCV induces lysosomal degradation of HNF-1 ␣ protein and how HNF-1 ␣ pro- tein is selectively downregulated by HCV ...the HCV NS5A protein is responsible for the HCV-in- duced ...
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Interaction of Hepatitis C Virus Nonstructural Protein 5A with Core Protein Is Critical for the Production of Infectious Virus Particles
... recombinant NS5A have demonstrated that NS5A is composed of three domains ...(52). Domain I is relatively con- served among HCV genotypes compared to domains II and ...conserved ...
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JBC-2005-Huang-36417-28.pdf
... the NS5A sequence to gain insight into NS5A structure or function, we asked whether the NS5A binding spec- ificity was ...for NS5A and provide additional clues into possible virus-host ...
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Crystal Structure of a Novel Dimeric Form of NS5A Domain I Protein from Hepatitis C Virus
... infectious HCV particles have provided further insight into the multiple roles of NS5A, which now includes participation in virion particle assembly by means of associa- tion of NS5A with ...
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Hepatitis C Virus NS5A Targets Nucleosome Assembly Protein NAP1L1 To Control the Innate Cellular Response
... of NS5A is required for NAP1L1 ...interaction, NS5A from JFH1 was divided in three domains, domain I (D1) (corre- sponding to nucleotides [nt] 2003 to 2189), domain II (D2) (nt 2226 to ...
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JBC-2012-Lim-30861-73.pdf
... all HCV genotypes, with the exception of Cys-39 in genotype 4A (28), which led us to hypothesize that they must be essential for the HCV life ...influence HCV RNA replication by affecting the ...
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Evasion of Superinfection Exclusion and Elimination of Primary Viral RNA by an Adapted Strain of Hepatitis C Virus
... between NS5A and NS5B are highly infectious. A successful model to study HCV superin- fection exclusion and viral mutations necessary to overcome the block requires that we (i) use a highly ...
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Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins
... the NS5A RNA-binding activity and its target sites in the genome will be facilitated by a description of the NS5A-RNA ...to NS5A. The minimal RNA-binding domain of NS5A consists of ...
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Over-expression and characterization of NS3 and NS5A of Hepatitis C virus genotype 3a
... [20,21]. NS5A also has significant role in the process of lipid accumulation that finally leads to stea- tosis ...of NS5A′ inhibitors is continuing and so far only one inhibitor “Daclatasvir” is available ...
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NS5A Domain 1 and Polyprotein Cleavage Kinetics Are Critical for Induction of Double Membrane Vesicles Associated with Hepatitis C Virus Replication
... infectious HCV particles (re- viewed in reference 10). The minimal HCV replicase comprises the remaining nonstructural proteins NS3, NS4A, NS4B, NS5A, and NS5B ...helicase domain formed by the ...
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Prevalence of hepatitis C virus (HCV) variants resistant to NS5A inhibitors in naïve patients infected with HCV genotype 1 in Tunisia
... the domain I of NS5A protein and have been shown to block phosphorylation of NS5A, which is essential for viral RNA replication and inhibits the assembly and release of viral particles [15, ...
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Calcium-Dependent Calpain Proteases Are Implicated in Processing of the Hepatitis C Virus NS5A Protein
... the HCV-1a NS5A protein. To achieve efficient expression of the HCV NS5A protein, we used HSV-1-based amplicon vec- tors ...modified HCV structural proteins ...the NS5A ...
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HCV NS5A region and response to pegylated interferon alpha plus ribavirin therapy: mutational analyses in patients with chronic HCV infection
... for HCV genotype 1 infected patients than patients infected with HCV genotype 2 and genotype 3 (Fried et ...with HCV genotype 1 could be its efficient replication ability enabling it to establish ...
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Response to combination therapy of HCV 3a infected Pakistani patients and the role of NS5A protein
... against HCV, 20 patients attained sustained virolo- gical response, 3 patients exhibited an end of treatment response while four patients were non- ...entire NS5A sequences of all HCV 3a isolates did ...
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Activation of the N-Ras–PI3K–Akt-mTOR Pathway by Hepatitis C Virus: Control of Cell Survival and Viral Replication
... full-length HCV replicon cell ...full-length HCV replicon ...(P-Akt). HCV-replicating SFL-2 and SFL-3 cells and control Huh7 and T1 cells were incubated in the presence or absence of serum, and ...
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Inhibition of Hepatitis C Virus Production by Aptamers against the Core Protein
... extracellular HCV RNA was measured by real-time ...by HCV-infected hepatocytes. HCV-infected ...with HCV core protein in the HCV-infected hepatocytes. HCV-infected ...
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Antigenic Heterogeneity of the Hepatitis C Virus NS5A Protein
... with HCV genotype 1b (sequence accession number ...the HCV genotype 1b sequence with the GenBank accession number ...five HCV-positive serum ...for HCV-specific antigenic reactivity by EIA ...
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Regulation of Hepatitis B Virus Replication by the Phosphatidylinositol 3-Kinase-Akt Signal Transduction Pathway
... HCV NS5A protein activates Akt and inhibits HBV gene ...and HCV share similar transmission routes, coinfection of the two viruses is frequently diagnosed ...chronic HCV infection frequently ...
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Polo-Like Kinase 1 Is Involved in Hepatitis C Virus Replication by Hyperphosphorylating NS5A
... with NS5A in the replicon ...and NS5A-HA or GST-HA-expressing vectors and used them for coimmuno- precipitation ...of NS5A- HA, the anti-HA antibody coprecipitated both NS5A and ...between ...
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