heat-labile enterotoxin B-subunits from human isolates of Escherichia coli
In silico Pulsed Field Gel Electrophoresis and Molecular characterization of Escherichia coli
... Sixty-five isolates were analyzed for virulence, antibiotic resistance and adherence genes by in silico ...classify Escherichia coli ...65 isolates, 3 isolates were harbouring ...
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Vaccine potential of LenA and LcpA proteins of Leptospira interrogans in combination with Escherichia coli heat-labile enterotoxin, B subunit (LTB)
... velopment of vaccines against leptospiral infections. LenA and LcpA proteins have been characterized as potential virulence factors. LcpA (leptospiral com- plement regulator-acquiring protein A) is a surface protein that ...
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Protection against Lethal Leptospirosis after Vaccination with LipL32 Coupled or Coadministered with the B Subunit of Escherichia coli Heat-Labile Enterotoxin
... ited by the coadministration of the rLTB and rLipL32 proteins. Similar results were found for LipL32 coupled to the B subunit of CT (CTB), which induced higher antibody titers than those elic- ited by treatment ...
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Human Papillomavirus Virus-Like Particles Are Efficient Oral Immunogens when Coadministered with Escherichia coli Heat-Labile Enterotoxin Mutant R192G or CpG DNA
... Certain human papillomaviruses (HPVs) cause most cervical cancer, which remains a significant source of morbidity and mortality among women ...large-scale human vaccination, the oral immu- nogenicity of HPV ...
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Intranasal Immunization of Mice with CpG DNA Induces Strong Systemic and Mucosal Responses That Are Influenced by Other Mucosal Adjuvants and Antigen Distribution
... hepatitis B surface antigen; CT, cholera toxin; CTB, subunit of cholera toxin; LT, Escherichia coli heat labile enterotoxin; LTK63, nontoxic derivative of Escherichia ...
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Adaptation of the staphylococcal coagglutination technique for detection of heat labile enterotoxin of Escherichia coli
... A total of 15 toxigenic and 61 nontoxigenic isolates were tested by the staphylococcal coagglutination technique in a coded fashion and found to be in agreement with previous results of [r] ...
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Expression of Escherichia coli heat labile enterotoxin B subunit in transgenic tomato (Solanum lycopersicum L ) fruit
... Binding assay of LTB protein to GM1-ganglioside. GM1-ganglioside has been shown to be the recep- tor for biologically active LTB protein, and the LTB pentameric structure is required for appreciable receptor binding. In ...
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Specificity of Escherichia coli Heat-Labile Enterotoxin Investigated by Single-Site Mutagenesis and Crystallography
... Figure 1. pLTB structure and interactions. Residues and features of special importance for this work are marked, with residues coloured in groups, as discussed in Section 2. (a) pLTB (PDB ID: 2XRQ [10] ) shown in surface ...
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Enzyme linked immunosorbent assay to measure antibodies to purified heat labile enterotoxins from human and porcine strains of Escherichia coli and to cholera toxin: application in serodiagnosis and seroepidemiology
... Serum immunoglobulin G antibodies to purified heat-labile enterotoxin LT from human LTh and porcine LTp Escherichia coli strains and cholera enterotoxin CT were measured by an enzyme-lin[r] ...
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Rapid detection by a coagglutination test of heat labile enterotoxin in cell lysates from blood agar grown Escherichia coli
... Cell lysates from human and animal ETEC strains grown on blood agar were compared for LT production by the coa-test, with staphylococci coated with anti-LT antibodies, and by the CHO cel[r] ...
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Plasmid Vectors Encoding Cholera Toxin or the Heat-Labile Enterotoxin from Escherichia coli Are Strong Adjuvants for DNA Vaccines
... and B subunit coding sequences (nor any other regions) of the CT and LT ...and B cells) followed by endosome acidification and likely degradation of the DNA into CpG-containing oligonucleotides ...seen ...
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Functional Pentameric Formation via Coexpression of the Escherichia coli Heat-Labile Enterotoxin B Subunit and Its Fusion Protein Subunit with a Neutralizing Epitope of ApxIIA Exotoxin Improves the Mucosal Immunogenicity and Protection against Challenge by Actinobacillus pleuropneumoniae
... LTB subunits have shown that the bac- terial subunit can assemble into a functional pentameric pro- tein under physiological conditions of eukaryotes, including plants and yeast (18, 35, ...LTB subunits, ...
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Heat Labile Enterotoxin IIa, a Platform To Deliver Heterologous Proteins into Neurons
... the B subunit, thereby noncovalently anchoring the A subunit and B subunits to create the holotoxin ...the heat-labile enterotoxins (LTs) of Escherichia coli: LTI, LTIIa, ...
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Solid Phase Microtiter Radioimmunoassay Blocking Test for Detection of Antibodies to Escherichia coli Heat Labile Enterotoxin
... Again the actual antibody titer of these sera was considerably higher in the RIA blocking test than the adrenal cell neutralization assay; however, the relative correlation of the two te[r] ...
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Comparison of methods to detect Escherichia coli heat labile enterotoxin in stool and cell free culture supernatants
... In addition, the ELISA of stool supernatants detected more samples known to be positive by YAC assay of pooled culture supernatants than did the ELISA of culture supernatants, possibly o[r] ...
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Automatic and manual latex agglutination tests for measurement of cholera toxin and heat labile enterotoxin of Escherichia coli
... M glycine-NaOH buffer This paper deals with automatic and manual Dow latex suspension inM0.05 8.2 containing 0.05 sodium chloride was mixed latex agglutination tests for cholera toxin CT[r] ...
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Modified Elek test for detection of heat labile enterotoxin of enterotoxigenic Escherichia coli
... As shown in Table 3, the results of the modified Elek test on Biken agar 2 correlated well with those of CHO cell assay and passive immune hemolysis.. A total of 161 strains gave consist[r] ...
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Antibody against recombinant heat labile enterotoxin B subunit (rLTB) could block LT binding to ganglioside M1 receptor
... E. coli cell was analyzed on 12% denaturized polyacrylamide gel ...buffer B (PBS containing 8M urea) followed by incubation at room temperature for 45 min and centrifuged (5000 g, 10 ...
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Simultaneous detection of Escherichia coli heat stable and heat labile enterotoxin genes with a single RNA probe
... A single RNA probe was synthesized and used to detect simultaneously the methanol-soluble heat-stable enterotoxin and heat-labile enterotoxin genes in Escherichia coli strains.. The resu[r] ...
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GM1 erythroimmunoassay for detection and titration of Escherichia coli heat labile enterotoxin
... Method for the detection and dosage of the Escherichia coli heat-labile enterotoxin by an enzyme-linked immunosorbent assay using a new simple kit test.. Production of "Escherichia coli"[r] ...
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