HEK 293 cells
Genetic Determinants of Cell Type-Specific Poliovirus Propagation in HEK 293 Cells
... neuronal cells can be reduced by introducing appropriate nucleotide substitutions into the viral ...identified HEK 293 cells as a novel system for the study of neuron-specific replication ...
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Sodium dependent norepinephrine induced currents in norepinephrine transporter transfected HEK 293 cells blocked by cocaine and antidepressants
... (293-hNET cells) was expanded and analyzed for expression of NET by immunoblotting, radioligand binding and radiolabeled flux ...1A, 293-hNET cells express an 80 kDa immunoreactive species ...
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Plasticity of the HEK-293 cells, related to the culture media, as platform to produce a subunit vaccine against classical swine fever virus
... B cells, as well as for the change of isotype and maturation of the antibody’s ...B cells and long-lived plasma cells (Henn et ...by HEK-293 cells growing in a serum-free sus- ...
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Opposite temperature effect on transport activity of KCC2/KCC4 and N(K)CCs in HEK 293 cells
... tems. HEK-293 cells represent the preferred mammalian expression system ...in HEK-293 iden- tified a marked increase in transport activity when shift- ing the cell line from room ...
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Effects of receptor tyrosine kinase inhibitors on VEGF165a and VEGF165b stimulated gene transcription in HEK 293 cells expressing human VEGFR2
... HEK-293 cells expressing the human VEGFR2 and an NFAT reporter gene were provided by Promega ...NFAT cells were maintained in DMEM media supplemented with 10% FCS and ...
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Zeolite catalysts for nitric oxide production
... Nitric oxide catalytic tests were performed in the same way as the acid sites, as well as further studies that considered copper leaching, toxicology of HEK 293 cells, NO production from[r] ...
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Detailed comparison of expressed and native voltage-gated proton channel currents
... HEK-293 cells and in COS-7 cells, two mammalian cell ...non-transfected HEK-293 cells have voltage dependence like that of other native proton currents ...
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Inosine binds to A3 adenosine receptors and stimulates mast cell degranulation
... accumulates to > 1 mM levels in ischemic tissues, triggers mast cell degranulation. Inosine was found to do the following: (a) compete for [125I]N6-aminobenzyladenosine binding to recombinant rat A3 adenosine ...
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Prokop, Stefan (2006): Funktionelle Charakterisierung der gamma-Sekretasekomponente PEN-2. Dissertation, LMU München: Medizinische Fakultät
... above-described cells with the highly potent and specific proteasome inhibitor clasto-lactacystin -lactone ...control cells or of the residual endoge- nous PEN-2 in the PEN-2 knockdown cells ...
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Merkel Cell Polyomavirus Small T Antigen Drives Cell Motility via Rho-GTPase-Induced Filopodium Formation
... to cells expressing ...expressing cells compared to EGFP-STΔ95–111-, EGFP-ST102A-, and EGFP-ST103- FIG 3 Screening of actin-associated proteins suggests MCPyV ST expression induces filopodium forma- ...(A) ...
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Deubiquitination of type 2 iodothyronine deiodinase by von Hippel–Lindau protein–interacting deubiquitinating enzymes regulates thyroid hormone activation
... in HEK-293 cells are targeted by the ubiquitin-proteasome system ...in HEK-293 ...these cells was not higher than in a human mesothe- lioma cell line that endogenously expresses ...
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Evidence that the β-catenin Nuclear Translocation Assay Allows for Measuring Presenilin 1 Dysfunction
... HEK 293 cell lines stably expressing either wt or mt PSEN1 were analyzed for PSEN1 expres- ...selected. HEK 293 cells are highly appropriate to evaluate the ef- fect of PSEN1 mutations ...
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Identification and Characterization of Nuclear and Nucleolar Localization Signals in the Adeno-Associated Virus Serotype 2 Assembly-Activating Protein
... HeLa cells and the signal intensi- ties in many AAP2 mutants were lower than those from the wild type to various ...from HEK 293 cells transfected with either the wild-type or mutant ...
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Roupelieva, Maria (2005): ORFeome-based arrays in eukaryotic expression vectors - a new approach to screen for the function of viral proteins: LANA-1 meets the mediator. Dissertation, LMU München: Fakultät für Biologie
... Subsequently, it was evaluated whether LANA-1 would stimulate activated promoters independently of the transcription factor binding site. A similar set of luciferase reporter vectors as in Fig. 13 was used and stimulated ...
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Stress-Inducible Alternative Translation Initiation of Human Cytomegalovirus Latency Protein pUL138
... MRC5 cells were cultured for 18 h in fed or stressed conditions and nucleofected with molar equivalents of pFL-cDNA constructs and analyzed for FL expression 6 h ...transfected cells to that of cDNA derived ...
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The p60-S6K1 isoform of ribosomal protein S6 kinase 1 is a product of alternative mRNA translation
... mutant HEK-293 cells targeted with anti-p85/p70-S6K1 gRNA/Cas9 to shut off expression of the main S6K1 isoforms, p85 and ...mutated HEK-293 clones after CRISPR/Cas9 ...+ ...
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Identification and Functional Comparison of Seven-Transmembrane G-Protein-Coupled BILF1 Receptors in Recently Discovered Nonhuman Primate Lymphocryptoviruses
... using cells that have been cotransfected with different BILF1 receptors expressing distinct epitope tags (c-myc and ...HA). HEK-293 cells were transfected with either HA-tagged EBV BILF1, ...
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Follicle-stimulating hormone (FSH) activates extracellular signal-regulated kinase phosphorylation independently of beta-arrestin- and dynamin-mediated FSH receptor internalization
... Sertoli cells in the testis, and granulosa cells in the ovarian follicle ...Sertoli cells to the hormone ...in HEK 293 cells tran- siently expressing the FSH-R, by enhancing ...
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Pegylated leptin antagonist with strong orexigenic activity in mice is not effective in chickens
... in HEK- 293 cells (Hen et ...1). HEK-293 cells expressing exogenous cLEPR and luciferase reporter genes were stimulated with human leptin and with interferon αA (IFNA2), both ...
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Mitina, Olga (2005): Src kinases and Flt3: phosphorylation, interference with receptor maturation and mechanism of association. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... in HEK-293 ...3 cells, cells were starved in the absence of IL-3 for 12 hours and then stimulated with FL for 10 minutes to induce Flt3 ...control, HEK-293 lysates con- taining ...
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