Hepatoma cell
Production of infectious duck hepatitis B virus in a human hepatoma cell line.
... 1736 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The differentiated human hepatoma cell line Hep-G2 was transfected with cloned duck hepatitis B virus DHBV DNA.. In[r] ...
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Niclosamide suppresses hepatoma cell proliferation via the Wnt pathway
... Methods: We performed an MTS assay to determine whether Wnt3a stimulated proliferation of Huh-6 and Hep3B human hepatoma cell lines after 72 hours of incubation with Wnt3a in serum-free medium. The cells ...
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Differentiation-specific transcriptional regulation of the hepatitis B virus large surface antigen gene in human hepatoma cell lines.
... The observation that the relative activity level of the large surface antigen promoter was 5 to 90 times higher in differentiated hepatoma cell lines than in the dedifferentiated hepatom[r] ...
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Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines
... stem cell conditioned culture system, we tested three human hepatoma cell lines, PLC/ PRF/5, MHCC97H and ...neous cell aggregation. The three cell lines could form clo- nal nonadherent ...
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Evaluation of the anticancer potential of six herbs against a hepatoma cell line
... The stock solutions of the herb extracts in DMSO were diluted with DMEM to the desired concentrations (10– 500 μg/mL). The maximum concentration of the 50% ethanol-water extracts in each test was 500 μg/mL, such that the ...
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THE BIOLOGICAL EFFECT OF B SITOSTEROL ON HEPATOMA CELL HEPG2 TRANSFECTED WITH HBX GENE
... in cell proliferation reported of Hela ...on hepatoma cell line transfected with HBx and its effect on hepatocellular ...and cell adhesion molecule, cell-cycle regulation, and tumor ...
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Effect of IRAK1 on Apoptosis and Necroptosis of Hepatoma Cell Line SK Hep1
... Cell lines and main reagents. The human hepatoma cell line SK-Hep1 was pur- chased from the cell bank of the Chinese Academy of Sciences. Necrostatin-1 (Nec-1) was purchased from Sigma, USA, ...
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Permissiveness of human hepatoma cell lines for HCV infection
... each hepatoma cell line could be attributed to entry factors already shown to modulate HCV permissiveness in other hepa- toma cell lines [25,32,48-51], we examined the expres- sion of four essential ...
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PCK1 negatively regulates cell cycle progression and hepatoma cell proliferation via the AMPK/p27Kip1 axis
... The CRISPR/Cas9 plasmids lentiCRISPR v2, pMD2.G, and psPAX2 were kindly provided by Prof. Ding Xue from the School of Life Sciences, Tsinghua University (Beijing, China). Single-guide RNAs targeting human PCK1 were ...
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Original Article Withaferin A inhibits hepatoma cell proliferation through induction of apoptosis and cell cycle arrest
... sis) (P<0.05). But, the apoptosis in L-02 cell was not significantly increased (Figure 2D). In addition, we examined the effect of WFA on cell cycle distribution in liver cancer cells. Hepa- tocarcinoma ...
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Original Article OCT4 expression maintained cancer stem-like properties in hepatoma cell line
... stem cell like clones and found Holoclone may be a promising candidate for its superior proliferation and anti-apoptosis ...HepG2 cell while it showed higher than its parental cell after ...parental ...
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State of hepatitis B viral DNA in a human hepatoma cell line.
... Results are shown for PM-2 [32PJDNA plus 10 pg of calf thymus DNA lane b and nick-translated plasmid HBV [32PJDNA plus 10 ug of calf thymus DNA lane c after each had been digested with e[r] ...
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Connexin32 regulates hepatoma cell metastasis and proliferation via the p53 and Akt pathways
... Hepatocellular carcinoma (HCC) progresses rapidly and is frequently associated with vascular invasion, metastasis, recurrence, and poor prognosis. The expression of connexin32 (Cx32) is frequently downregulated in HCC ...
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Polypeptides of hepatitis B virus surface antigen produced by a hepatoma cell line.
... As the relative proportions of p20 and p23 appear to be the DISCUSSION same in HBsAg particles from both PLC/PRF/ We have iradiolabeled and purified the HBsAg 5 cells and serum, these tw[r] ...
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Original Article miR-143 down-regulates TLR2 expression in hepatoma cells and inhibits hepatoma cell proliferation and invasion
... Abstract: Hepatoma is a tumor with high degree of ...different hepatoma tissues, and that miR-143 expressions contribute disease ...a cell cycle arrest though TLR and NF-κB ...
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Activation and nuclear translocation of ERK in response to ligand dependent and independent stimuli in liver and gill cells from rainbow trout
... the cell type investigated ...the cell-type specificity of ERK activation and subcellular distribution, the responses to the same stimuli were also studied in a trout hepatoma cell line as ...
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Roles of dopamine receptors and their antagonist thioridazine in hepatoma metastasis
... treated hepatoma cell lines SNU449, LM3, and Huh7 with THIO and compared them with normal hepatic cell L02, we found that THIO inhibited HCC cell growth in a dose-dependent fashion, but had ...
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Robust Production of Infectious Hepatitis C Virus (HCV) from Stably HCV cDNA-Transfected Human Hepatoma Cells
... robust cell culture system that con- tinuously produces and secretes infectious HCV into the cul- ture medium by stable human hepatoma cell ...Huh7 cell lines were isolated following DNA ...
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Sgo1 is a potential therapeutic target for hepatocellular carcinoma
... reduced cell viability of hepatoma cell lines including HuH7, HepG2, Hep3B, and ...that hepatoma cells were delayed and ultimately die in mitosis in the absence of ...contrast, cell ...
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Apoptosis of Hepatitis B Virus-Infected Hepatocytes Prevents Release of Infectious Virus
... host cell, especially at the onset of an acute HBV infection, to facilitate HBV particle ...HBV-producing hepatoma cell lines or HBV-infected PHH, analyzed virus particles released into the ...
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