Heptad Repeat
Oligomerization of the hydrophobic heptad repeat of gp41.
... hydrophobic heptad repeat of gp41 to direct protein oligomerization, we have cloned this region of HIV-1 gp41 into the bacterial expression vector pRIT2T, a plasmid which encodes the N-terminal region of ...
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A New Class of Receptor for Herpes Simplex Virus Has Heptad Repeat Motifs That Are Common to Membrane Fusion Proteins
... indicate that the hfl-B5 sequence encodes a type II integral membrane protein that has an extracellular C-terminal heptad repeat (Fig. 2). There are no published reports to characterize the predicted ...
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Spring-Loaded Heptad Repeat Residues Regulate the Expression and Activation of Paramyxovirus Fusion Protein
... two heptad repeat regions (HRA and HRB) that are important in membrane fusion and can be targeted by antiviral ...the heptad repeat nor conserved among the paramyxoviruses were key regulators ...
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A hydrophobic heptad repeat of the core protein of woodchuck hepatitis virus is required for capsid assembly.
... The capsid particle of hepadnaviruses is assembled from its dimer precursors. However, the mechanism of the protein-protein interaction is still poorly understood. A small region in the capsid protein of woodchuck ...
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Interaction of Peptides with Sequences from the Newcastle Disease Virus Fusion Protein Heptad Repeat Regions
... Typical of many viral fusion proteins, the sequence of the Newcastle disease virus (NDV) fusion protein has several heptad repeat regions. One, HR1, is located just carboxyl terminal to the fusion peptide, ...
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Peptides Corresponding to the Heptad Repeat Motifs in the Transmembrane Protein (gp41) of Human Immunodeficiency Virus Type 1 Elicit Antibodies to Receptor-Activated Conformations of the Envelope Glycoprotein
... the heptad repeat regions of the ...the heptad repeat domains in the fusion-intermediate, such as the coiled-coil trimer for the N heptad repeat, are needed to evaluate the ...
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Functional Characterization of Heptad Repeat 1 and 2 Mutants of the Spike Protein of Severe Acute Respiratory Syndrome Coronavirus
... Despite the fact that the SARS-CoV S protein shares little amino acid similarity with other CoVs, the putative S2 domain of the SARS-CoV also contains two predicted amphipathic ␣ -helical, 4-3 heptad repeat ...
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Residues in the Heptad Repeat A Region of the Fusion Protein Modulate the Virulence of Sendai Virus in Mice
... the heptad repeat A region of the ectodomain, a region of the protein known to regulate F protein ...the heptad repeat A region of the F protein modulate the virulence of Sendai virus in mice ...
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Mutations in the Fusion Peptide and Adjacent Heptad Repeat Inhibit Folding or Activity of the Newcastle Disease Virus Fusion Protein
... Paramyxovirus fusion proteins have two heptad repeat domains, HR1 and HR2, which have been implicated in the fusion activity of the protein. Peptides with sequences from these two domains form a ...
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Heptad Repeat-Derived Peptides Block Protease-Mediated Direct Entry from the Cell Surface of Severe Acute Respiratory Syndrome Coronavirus but Not Entry via the Endosomal Pathway
... Severe acute respiratory syndrome (SARS) coronavirus (SCoV) is a causative agent of life-threatening SARS (4, 7, 15, 31). Although the first outbreak of SARS was stamped out, an effective antiviral drug is still required ...
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Examination of a Fusogenic Hexameric Core from Human Metapneumovirus and Identification of a Potent Synthetic Peptide Inhibitor from the Heptad Repeat 1 Region
... Paramyxoviruses are a leading cause of childhood illness worldwide. A recently discovered paramyxovirus, human metapneumovirus (hMPV), has been studied by our group in order to determine the structural relevance of its ...
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Selection with a Peptide Fusion Inhibitor Corresponding to the First Heptad Repeat of HIV-1 gp41 Identifies Two Genetic Pathways Conferring Cross-Resistance to Peptide Fusion Inhibitors Corresponding to the First and Second Heptad Repeats (HR1 and HR2) of gp41
... gp41 heptad repeat regions that affect fusogenicity (12, 43, 54, 68) and sensitivity to antibody neutral- ization or peptide inhibition (4, 8, 14, 15, 34, 51, 69, 72, ...
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Mutations of Glu560 within HIV 1 Envelope Glycoprotein N terminal heptad repeat region contribute to resistance to peptide inhibitors of virus entry
... C‑terminal heptad repeat regions (HR1 and HR2, respectively) of gp41 can inhibit HIV‑1 infection in a dominant negative manner by interfering with refolding of the viral HR1 and HR2 to form a six‑helix ...
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Coronavirus Escape from Heptad Repeat 2 (HR2)-Derived Peptide Entry Inhibition as a Result of Mutations in the HR1 Domain of the Spike Fusion Protein
... two heptad repeat (HR) regions play a key role: HR1, positioned C-terminal of a hy- drophobic fusion peptide, and HR2, located N-terminal of the transmembrane ...
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Mutations of Gln64 in the HIV-1 gp41 N-Terminal Heptad Repeat Render Viruses Resistant to Peptide HIV Fusion Inhibitors Targeting the gp41 Pocket
... strains still retained substantial infectivity, we tested their suscep- tibility to the selected peptide HIV fusion inhibitors, including T20, C34, CP32M, and C52L. We found that the viruses with Q64 mutations in the ...
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Heptad Repeat 2 in Herpes Simplex Virus 1 gH Interacts with Heptad Repeat 1 and Is Critical for Virus Entry and Fusion
... Viral fusion glycoproteins are responsible for execution of fusion between the viral envelope and cell membranes. Key structural elements of class 1 viral fusion glycoproteins (e.g., the influenza virus hemagglutinin, ...
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Heptad Repeat 2-Based Peptides Inhibit Avian Sarcoma and Leukosis Virus Subgroup A Infection and Identify a Fusion Intermediate
... distinct heptad repeat domains within the transmembrane ...two heptad repeat domains of the avian sarcoma and leukosis virus subgroup A (ASLV-A) TM subunit of the envelope protein were ...
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A Heptad Repeat in Herpes Simplex Virus 1 gH, Located Downstream of the α-Helix with Attributes of a Fusion Peptide, Is Critical for Virus Entry and Fusion
... Entry of herpes simplex virus 1 (HSV-1) into cells occurs by fusion with cell membranes; it requires gD as the receptor binding glycoprotein and the trigger of fusion, and the trio of the conserved glycoproteins gB, gH, ...
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Inhibition of Murine Leukemia Virus Envelope Protein (Env) Processing by Intracellular Expression of the Env N-Terminal Heptad Repeat Region
... HEK293 cells were cotransfected with 1.5 g each of expression vectors for HIV-1 envelope protein pdl1443, amphotropic MLV envelope protein pCAETr Ampho MLV, or VSV-G pVSV-G, along with [r] ...
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Structure of the Fusion Core and Inhibition of Fusion by a Heptad Repeat Peptide Derived from the S Protein of Middle East Respiratory Syndrome Coronavirus
... Middle East respiratory syndrome coronavirus (MERS-CoV) recently emerged as a severe worldwide public health concern. The virus is highly pathogenic, manifesting in infected patients with an approximately 50% fatality ...
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