heterogeneous nuclear ribonucleoprotein C
Functional Interaction of Heterogeneous Nuclear Ribonucleoprotein C with Poliovirus RNA Synthesis Initiation Complexes
... hnRNP C decreased translation and replication but failed to completely deplete the extracts of hnRNP C (data not ...hnRNP C found in HeLa cytoplasmic extracts ...hnRNP C in ex- tracts while ...
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Identification and cloning of a novel heterogeneous nuclear ribonucleoprotein C-like protein that functions as a transcriptional activator of the hepatitis B virus enhancer II.
... The cDNA clone of the protein, designated E2BP, was isolated from a Agtll expression library constructed from the hepatoma cell line HuH-6 which was screened with a binding site probe de[r] ...
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Heterogeneous Nuclear Ribonucleoprotein L Interacts with the 3′ Border of the Internal Ribosomal Entry Site of Hepatitis C Virus
... hepatitis C virus (HCV) RNA occurs by internal entry of a ribosome into the 5 * nontranslated region in a cap-independent ...be heterogeneous nuclear ribonucleoprotein L (hnRNP ...
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Role of human heterogeneous nuclear ribonucleoprotein C1/C2 in dengue virus replication
... Clear lysates or immunoprecipitated samples prepared from mock-infected and DENV-infected cells that were untransfected or transfected with siRNA were mixed with 4× loading buffer [50 mM Tris–HCl (pH 6.8), 2% SDS, 0.1% ...
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Regulation of Notch Signaling by the Heterogeneous Nuclear Ribonucleoprotein Hrp48 and Deltex in Drosophila melanogaster
... Notch protein is uniformly expressed throughout the wing disc with the highest protein level on the apical surface (Fehon et al. 1991). Overexpression of Dx with ptc-GAL4 led to relocalization of Notch in cytoplasmic ...
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SYNCRIP, a Member of the Heterogeneous Nuclear Ribonucleoprotein Family, Is Involved in Mouse Hepatitis Virus RNA Synthesis
... SYNCRIP binds to both MHV 5 ⴕ -UTR and c5 ⴕ -UTR in vitro. To test whether SYNCRIP is able to bind to MHV UTR, we performed in vitro binding analysis. His-tagged, recombinant SYNCRIP was expressed in Escherichia coli, ...
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A Yeast Heterogeneous Nuclear Ribonucleoprotein Complex Associated With RNA Polymerase II
... they indicate that Nrd1p and pol II function in the same pathway in vivo, most likely through direct interaction. NRD1-NAB3 interaction: We have also demonstrated genetic and physical interactions between RNA-binding ...
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Heterogeneous Nuclear Ribonucleoprotein A2 Participates in the Replication of Japanese Encephalitis Virus through an Interaction with Viral Proteins and RNA
... the nuclear and cytoplasmic fractions were detected by immunoblotting with rabbit anti-core PAb and mouse anti-hnRNP A2 MAb (DP3B3), ...as nuclear and cytoplasmic markers, ...
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A Novel Heterogeneous Nuclear Ribonucleoprotein-Like Protein Interacts with NS1 of the Minute Virus of Mice
... part C the serum was prein- cubated with 1 mg of synthetic NSAP1 peptide per ml and in part D the serum was preincubated with 100 mg of synthetic NSAP1 peptide per ...
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Mutational analysis of a heterogeneous nuclear ribonucleoprotein A2 response element for RNA trafficking
... cleotide treatment on MBP RNA transport. A–C, antisense-treated cells. A, RNA staining is primarily restricted to the perinuclear cytoplasm with only one or two spots of staining away from the cell body ...
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Anti A2/RA33 autoantibodies are directed to the RNA binding region of the A2 protein of the heterogeneous nuclear ribonucleoprotein complex Differential epitope recognition in rheumatoid arthritis, systemic lupus erythematosus, and mixed connective tissue disease
... Purification of hnRNP-A2 deletion mutants. Recombinant pro- teins were prepared by resuspending the cell pellet obtained from 0.5 liter cultures in buffer A (25 mM Na-acetate, 2 M urea, 0.01% Triton X-100, pH 5.5) ...
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Heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a tissue biomarker for detection of early Hepatocellular carcinoma in patients with cirrhosis
... hnRNP K has been shown to be involved in a number of cellular functions [18,19]. It also exhibits specific binding and transactivation within the c-myc promoter [20,21]. In addition, p53 and hnRNP K cooperate to ...
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Nuclear heterogeneous nuclear ribonucleoprotein D is associated with poor prognosis and interactome analysis reveals its novel binding partners in oral cancer
... detectable nuclear hnRNPD immunostain- ing was observed in 86/106 cases ...moderate nuclear expression in epithelial cells of basal/supraba- sal ...Increased nuclear expression of hnRNPD was observed ...
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Heterogeneous nuclear ribonucleoprotein complexes from Xenopus laevis oocytes and somatic cells
... out at 0 ° -4 ° C. Cells grown in monolayer culture to subconfluent density were washed with PBS and collected with a cell scraper in RSB-100 (10 mM Tris, pH 7.4; 100 mM NaCl; 2.5 mM MgCl 2 ) containing 0.5% ...
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Heterogeneous Nuclear Ribonucleoprotein M Facilitates Enterovirus Infection
... and C-terminal cleavage proteins of hnRNP M contain at least one RRM and thus presumably have the ability to bind ...and C-terminal fragment persists at least until 5 h ...
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Translational upregulation of folate receptors is mediated by homocysteine via RNA heterogeneous nuclear ribonucleoprotein E1 interactions
... UV-cross-linked RNA-protein complexes at 4°C for 2 hours on a shaking platform. IgGsorb (200 µl) was then added and samples were incubated for 2 hours, followed by centrifugation at 13,600 g for 5 minutes at ...
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Key role of UBQLN2 in pathogenesis of amyotrophic lateral sclerosis and frontotemporal dementia
... ubiquilin family [12, 29]. UBQLN2 has a ubiquitin-like domain (UBL) in its N-terminal that can interact with the proteasome, and a ubiquitin-associated domain (UBA) on the C-terminal which recognizes the polyubi- ...
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The Extended C-Terminal Region of Influenza C Virus Nucleoprotein Is Important for Nuclear Import and Ribonucleoprotein Activity
... Influenza C virus NP binds multiple importin ␣ isoforms. Conventional nuclear import events rely on host importin ␣ /  ...for nuclear import has been well documented (16, 30, ...on nuclear ...
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Targeting a ribonucleoprotein complex containing the caprin-1 protein and the c-Myc mRNA suppresses tumor growth in mice: an identification of a novel oncotarget
... on c-Myc, cyclin D1, and cyclin D2 expression in carcinoma ...of c-Myc, cyclin D1, and cyclin ...antibodies. C. Tylophorine repressed the de novo protein syntheses of c-Myc and cyclins ...
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Specific Interaction of Heterogeneous Nuclear Ribonucleoprotein Particle U with the Leader RNA Sequence of Vesicular Stomatitis Virus
... Immunoprecipitation of VSV RNAs. In vitro, radiolabeled LS RNA was al- lowed to bind with either nuclear extract from HeLa cells or the bacterially expressed hnRNP U (U protein). Monoclonal antibody to U protein ...
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