High density cell culture
Original Article Development of a model system to analyze chondrogenic differentiation of mesenchymal stem cells
... Abstract: High-density cell culture is widely used for the analysis of cartilage development of human mesenchymal stem cells (HMSCs) in ...Several cell culture systems, as ...
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Autotrophic Growth of Paracoccus denitrificans in Aerobic Condition and the Accumulation of Biodegradable Plastics from CO2
... rpm. High cell density culture experiment was carried out using a glass jar fermenter (total volume 1000 mL; working volume 600 mL) equipped with a pH controller, PHC-2201 and a dissolved ...
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The Suppression of Medium Acidosis Improves the Maintenance and Differentiation of Human Pluripotent Stem Cells at High Density in Defined Cell Culture Medium
... at high density could push the regular cell culture capacity over its ...and cell-building materials for cell proliferation[34], producing lactic acid during the ...to ...
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Optimization of Medium Components Using Artificial Neural Networks
... inhibit cell growth when they are present above a certain ...batch culture media has not yielded high ...have high cell growth as well as high recombinant protein production, the ...
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Development of a simple and high-yielding fed-batch process for the production of porcine circovirus type 2 virus-like particle subunit vaccine
... for cell growth. This phenomenon referred to as the “cell density effect”, which has been extensively documented by others for insect and human cell production systems infected by viral ...
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Extracellular Matrix and Control of Proliferation of Vascular Endothelial Cells
... Bovine vascular endothelial cells plated at low cell density in the presence of high (10%) concentrations of serum and maintained on plastic tissue culture dishes proliferate slowly. If the ...
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The mammalian germline as a pluripotency cycle
... ES cell-like pluripotent stem cells in high-density culture and the presence of leukaemia inhibitory factor (LIF) (see Glossary, Box 1) (Bao et ...EG cell lines (Leitch et ...ES ...
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High yield expression of proteins in E coli for NMR studies
... report high yield target protein expre- ssion with Maltose binding protein (MBP) [19], Gluta- thione-S transferase (GST) [20], Thioredoxin fusion (TRX) [21], Hexahistidine His6-Tag [19,22], Small ubiquitin- ...
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Large-scale production of magnetosomes by chemostat culture of Magnetospirillum gryphiswaldense at high cell density
... and maximized magnetosome formation by Magnetospiril- lum gryphiswaldense strain MSR-1 at low dissolved oxygen concentration and carbon source limitation. pH-stat feed- ing is a feeding strategy based on a pH feedback ...
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Original Article Effect of ex vivo culture density on CXCR7 expression in human mesenchymal stem cells
... tissue culture surfaces among cells derived from tissues [6, 8, ...and cell confluencies following culture for various amounts of time to identify the effect of ex vivo culture conditions on ...
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Optimization of human corneal endothelial cell culture: density dependency of successful cultures in vitro
... endothelial cell-clusters were rinsed once in PBS and further dissociated with a brief treatment of TE for 5 minutes to obtain smaller ...The cell clumps were washed and collected after centrifugation at ...
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Electron Microscopy of the Intracellular Development of Bacteriophage MS2 in Escherichia coli
... coli C3000 culture 180 min after infection with MS2 under conditions of high cell density.. The majority of the cells has lysed.[r] ...
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Vol 3, No 10-12 (2014)
... embryonal-suspensor cell masses, while high densities result in the formation of small spherical cell ...of culture in L leptolepis indicated that small spherical aggregates proliferated in a ...
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Monitoring of fermentations with online capacitance sensor
... for high cell density cultivations and especially for mammalian cell culture, for which the sensor was already successfully applied (Zhang et ...with cell-free media, fed the ...
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Optimization of various factors affecting Agrobacterium-mediated transformation and regeneration of putative transgenic Malaysian MR219 rice with Bacillus SP 289 endo-β-1,3-1,4-glucanase gene
... Agrobacterium culture cell density ...Agrobacterium culture (30, 60, 90 and 120 minutes), subsequent duration of drying before co-cultivation (15, 30, 45, 60 and 90 minutes), and ...
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Experimental-ecological investigations onPhaeocystis poucheti (Haptophyceae): cultivation and waste water test
... After the maximum cell density is reached in the nutrient enriched culture medium, however, the single cell stage4s favoured, whilst the colony stage very quickly d[r] ...
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Channel Allocation Scheme in Cellular System
... the cell, G', with min {F (B, G’)} for channel borrowing, if the basic borrowing criterion is ...selected cell G' computes the set of its co-channel cells which are non-co-channel to B by comparing the ...
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Hemadsorption of Mumps Virus Examined by Light and Electron Microscopy
... The gap of low electron density normally separating the morphologically intact membrane of the tissue culture cell and that of the red blood cell at the binding site was replaced by newl[r] ...
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Scale-up bioprocess development for production of the antibiotic valinomycin in Escherichia coli based on consistent fed-batch cultivations
... a high glucose concentration [26]. As a high glucose concentra- tion is connected to a high metabolic activity, also oxy- gen limitation appears in such a zone at the typical high cell ...
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Isolation and Cultivation of a Newly-Discovered AstaxanthinRich Green Microalga - Haematococcus sp. Flotow Strain from Homeros Valley (Bornova Creek, Izmir, Turkey)
... species - H. pluvialis, H. lacustris, H. droebakensis and H. capensis with 11 strains have been cultivated in laboratory and commercial cultures. There have been several studies on H. pluvialis cultivation in Turkey ...
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