High-mobility group protein 2
High mobility group box protein 1 (HMGB1): an alarmin mediating the pathogenesis of rheumatic disease
... High-mobility group box protein 1 (HMGB1) is a non-histone nuclear protein that has a dual function. Inside the cell, HMGB1 binds DNA, regulating transcription and determining ...
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Neuroinflammation in Autism Spectrum Disorders: the Role of High Mobility Group Box 1 Protein
... of high mobility group box-1 protein (HMGB1), an inflammatory cytokine-like marker, and ASD-related symptoms incidence, prevalence and ...this protein can play a role in both ...
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Original Article Knockdown of high-mobility group nucleosome-binding protein 2 increases uropathogenic Escherichia coli invasion in human bladder epithelial cells through promoting actin cytoskeleton polymerization
... in UPEC adhesion and invasion in BECs, espe- cially the α3/β1 integrins [4]. To gain insight into whether α3/β1 integrins involve in mediat- ed changes in UPEC adhesion and invasion, we assayed the mRNA and ...
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Extracellular High-Mobility Group Box 1 Protein (HMGB1) as a Mediator of Persistent Pain
... whole-cell protein extracts showed ele- vated levels of HMGB1 protein in the spinal cord after the induction of nerve ligation (75), diabetic neuropathy (80) and bone cancer (79) (Table ...
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The role of high mobility group box chromosomal protein 1 expression in the differential diagnosis of hepatic actinomycosis: a case report
... Actinomycosis is a chronic, slowly progressive infection caused by the Gram-positive anaerobic bacterium Actinomyces israelii. A. israelii is part of the normal flora of the oropharynx, gastrointestinal tract, and female ...
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High Mobility Group Box Protein 1 (HMGB1): The Prototypical Endogenous Danger Molecule
... which resulted in the detection of extra- cellular HMGB1 as a delayed mediator after LPS stimulation. Systemic HMGB1 levels in vivo in mice started by 8 h and increased substantially from 16 to 32 h after LPS ...
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Members of the high mobility group B protein family are dynamically expressed in embryonic neural stem cells
... acid protein (GFAP) [27,28] and were highly proliferative, capable of extensive self-renewal and multipotent diffe- rentiation, generating astrocytes (GFAP+), neurons (βIII tubulin+) and oligodendrocytes (CNP+) ...
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The High Mobility Group Protein 1 Is a Coactivator of Herpes Simplex Virus ICP4 In Vitro
... and 2), lacked the basal Inr activity that was apparent when the crude DB fraction was used (lanes 3 and ...Fig. 2 were performed with equivalent amounts of TFIID, based on TBP content as determined by ...
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High mobility group box 1 protein, lipopolysaccharide binding protein, interleukin 6 and C reactive protein in children with community acquired infections and bacteraemia: a prospective study
... techniques, markedly lower HMGB1 levels were found. Hatada et al. [11] observed median HMGB1 levels of 4.5 ng/ml] in infected patients. HMGB1 levels of 7.7 ng/ml were measured by Yasuda et al. [28] in infected patients ...
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Characterization of an Entamoeba histolytica High-Mobility-Group Box Protein Induced during Intestinal Infection
... priate amounts of recombinant wild-type or mutant EhHMGB1 (0.25 to 4 M) in T4 DNA ligase buffer (New England Biolabs [NEB]) in a 20-l reaction volume. The DNA was then ligated with T4 DNA ligase (NEB; 40 units per ...
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Renal expression and serum levels of high mobility group box 1 protein in lupus nephritis
... block with 2% human AB sera for 30 minutes and an avidin/biotin-blocking step (Vector Laboratories Inc., Burlingame, CA, USA). The slides were thereafter incu- bated overnight with an affinity-purified monoclonal ...
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Potential Role for a β-Catenin Coactivator (High-Mobility Group AT–Hook 1 Protein) during the Latency-Reactivation Cycle of Bovine Herpesvirus 1
... g protein) was incubated with the anti-Flag M2 affinity gel with gentle rotation by a roller shaker for 2 h at ...magnetic protein A beads (catalog number S1425S; ...
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The N terminal domain of thrombomodulin sequesters high mobility group B1 protein, a novel antiinflammatory mechanism
... of protein A agarose bead solution) were added to protein mixtures (200 μl each) and samples were incubated for an additional 1 ...hour. Protein A agarose was harvested by centrifugation and the ...
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High Mobility Group Box Protein-1 Correlates with Renal Function in Chronic Kidney Disease (CKD)
... ferred to a new tube, mixed with 5X Laemmli Sample Buffer (BioRad, Her- cules, CA, USA), heated at 95°C for 5 min and spun down at 8160g for 5 min, in tabletop microfuge. 12.5 μL of heated sample (10 μL serum per lane) ...
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High Mobility Group box-1 (HMGB1) Protein As a Biomarker for Acute Cholecystitis
... The current study supports results from previous research investigating predictors of acute cholecystitis. C-reactive protein, as an acute phase reactant, has been proven to be a suitable diagnostic biomarker for ...
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HMGA2 expression in white adipose tissue linking cellular senescence with diabetes
... type 2 (T2D), the mechanisms by which an excess of adipose tissue contributes to the onset of the metabolic syndrome are as yet far away from being fully understood (Ahima ...A protein that is apparently ...
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The high mobility group protein HMO1 functions as a linker histone in yeast
... NaCl, 2 mM EDTA, 10 % glycerol, 5 % Triton X-100) containing 100 mM β-mercaptoethanol, ...(Roche). Protein concentration was measured using BCA Protein Assay Kit ...of protein was resolved on ...
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<p>Serum high mobility group box protein 1 (HMGB1) levels reflect clinical features of childhood hemophagocytic lymphohistiocytosis</p>
... (Figure 2). Among the patients with strikingly high HMGB1 levels, patient 6 had complicated mild leukoen- cephalopathy revealed by magnetic resonance imaging (MRI) and required exchange transfusion in ...
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High mobility group protein B1 is a predictor of poor survival in ovarian cancer
... analysis was semi-automated using Aperio ImageScope v11.2.0.780 software. The positive pixel count v9.1 algorithm settings were modified to provide accurate calling of expression levels (Iwp(high) at 140, ...
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Investigations into the interactions of the high mobility group box 1 protein and their toxicological relevance
... Δ30 protein acted in synergy with IL-1β to enhance IL-6 release in SFs (Chapter 4, Section ...∆30 protein appeared to be well folded as the peaks on the HSQC spectra were well resolved and ...IL-1β ...
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