High-resolution Melt Analysis
Rapid Detection and Identification of Mucormycetes in Bronchoalveolar Lavage Samples from Immunocompromised Patients with Pulmonary Infiltrates by Use of High Resolution Melt Analysis
... by high- resolution melt analysis (PCR/HRMA) to detect Rhizopus ...its high negative predictive value (99%), represents a fast and reliable tool for routine BAL sample screening for the ...
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Integrated Microfluidic Card with TaqMan Probes and High Resolution Melt Analysis To Detect Tuberculosis Drug Resistance Mutations across 10 Genes
... The TaqMan array card (TAC) is a customizable 384-well mi- crofluidic real-time PCR system that compartmentalizes each sample into 48 different PCRs simultaneously and has been ap- plied for the detection of multiple ...
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Genotyping of Chlamydophila psittaci by Real Time PCR and High Resolution Melt Analysis
... Human infection with Chlamydophila (Chlamydia) psittaci can lead to psittacosis, a disease that occasionally results in severe pneumonia and other medical complications. C. psittaci is currently grouped into seven avian ...
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Rapid Identification and Discrimination of Brucella Isolates by Use of Real Time PCR and High Resolution Melt Analysis
... in high rates of reproductive fail- ure and sterility in livestock, these agents are most commonly transmitted to humans through the consumption of unpasteur- ized milk products, direct contact with infectious ...
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High Resolution Melt analysis for mutation screening in PKD1 and PKD2
... In our facility, the price for one amplicon analysis with HRM is 1.52 $ (1.10 € ) compared to 4.64 $ (3.36 € ) with dHPLC [28]. In addition, HRM allows large fragment rearrangements detection in the same step. ...
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Rapid Detection of Multidrug Resistant Mycobacterium tuberculosis by Use of Real Time PCR and High Resolution Melt Analysis
... of IS6110 and the RRDR of rpoB from MTC. The IS6110 marker was analyzed using melt curve analysis (MCA) of the PCR amplicons to confirm that samples were MTC strains. MCA profiles for MTC strains contained ...
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Detection of Plasmodium Species by High Resolution Melt Analysis of DNA from Blood Smears Acquired in Southwestern Uganda
... of high-quality DNA, but such samples require careful transportation, handling, and storage (9, ...HRM analysis to compare Plasmodium species detection from blood smears and corresponding red blood cell ...
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Rapid Identification of Bacterial Pathogens in Positive Blood Culture Bottles by Use of a Broad Based PCR Assay Coupled with High Resolution Melt Analysis
... Nucleic acid amplification methods that target the bacteria- specific 16S rRNA gene can offer many advantages; the highly conserved sequences of the gene allow broad-based detection of almost any bacterial species, while ...
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Molecular Identification of Mycobacterium avium subsp silvaticum by Duplex High Resolution Melt Analysis and Subspecies Specific Real Time PCR
... s. Melt curve conditions were 85°C to 99°C at a ramp rate of ...HRM analysis was performed with temperatures from 87°C to 99°C with increases of ...HRM analysis, an indepen- dent-sample t test was ...
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Rapid High Resolution Melt Analysis of Cytauxzoon felis Cytochrome b To Aid in the Prognosis of Cytauxzoonosis
... C ytauxzoonosis is an emerging disease in domestic and wild felids in North and South America, caused by the tick-trans- mitted apicomplexan parasite Cytauxzoon felis (1–8). Cytauxzoo- nosis was originally thought to be ...
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Real Time PCR and High Resolution Melt Analysis for Rapid Detection of Mycobacterium leprae Drug Resistance Mutations and Strain Types
... time-HRM analysis provides simple, rapid, and robust classification and is suitable for comparison of ...highest resolution of strains within SNP subtypes has been achieved by VNTR strain typing (7, 15, 30, ...
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Speciation of common Gram negative pathogens using a highly multiplexed high resolution melt curve assay
... targets. High resolution melt analysis is an end-point qPCR detection method that provides greater multiplexing capability than probe based ...a high resolution melt ...
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A refined, rapid and reproducible high resolution melt (HRM) based method suitable for quantification of global LINE 1 repetitive element methylation
... High Resolution Melt analysis PCR (HRM PCR) is gaining acceptance as a robust method for measuring levels of methylation ...a high correlation between results obtained using HRM ...
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Simultaneous Detection of Colletotrichum acutatum and C gloeosporioides from Quiescently Infected Strawberry Foliage by Real Time PCR Based on High Resolution Melt Curve Analysis
... (RFLP) analysis [19] [20] and DNA fingerprinting by random amplifica- tion of polymorphic DNA (RAPD) [20] [21] [22] have all been used for detec- tion and differentiation of the Colletotrichum ...
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Differentiation of Solenopsis invicta social forms using high resolution melt PCR.
... staining. Analysis of the aligned Gp-9 nucleotide sequence data described above indicated that the 390 bp would digest to either two fragments of 200 and 189 bp (BB homozygotes) or four fragments of 200, 189, 117 ...
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Rapid Differentiation of Mycobacterium tuberculosis and M bovis by High Resolution Melt Curve Analysis
... A two-reaction approach for differentiating between M. bovis and M. tuberculosis was carried out using two real-time PCR assays followed by HRM analysis. Assay 1 consisted of the identification of the ...
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High Throughput Genotyping of Single Nucleotide Polymorphisms in the Plasmodium falciparum dhfr Gene by Asymmetric PCR and Melt Curve Analysis
... However, compared to those for the published TaqMan- based real-time PCR assay by Alker et al. (2, 3), our assay demonstrated greater sensitivity and specificity. Although the reported sensitivity of the TaqMan assay is ...
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Rapid identification of chloroquine and atovaquone drug resistance in Plasmodium falciparum using high resolution melt polymerase chain reaction
... PCR-HRM for the detection of atovaquone resistance Primers and probes were designed using Primer 3 soft- ware. The PCR was set up in a final volume of 10 μl con- taining 1 μl of DNA, 50 nM of cytbF primer (5'- ...
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High Resolution Melt PCR Analysis for Genotyping of Ureaplasma parvum Isolates Directly from Clinical Samples
... Although our HRM assay is highly effective at differentiating the serovars of U. parvum in cases of single-serovar colonization, it is important to note that it was not specifically designed as a de- tection tool for the ...
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Advances in 3D imaging and volumetric reconstruction of fluid and melt inclusions by high resolution X-ray computed tomography
... and melt inclusions are tiny pockets of fluid and melt trapped in natural and synthetic ...and melt inclusions within minerals, their shape and the volume fraction of their different phases is ...
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