High resolution melting analysis (HRM)
High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes
... average melting peaks, which allowed the easy identification and selection of the correct cloned segments or reassorted ...virus, High resolution melting ...
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The Use of High Resolution Melting Analysis for Detection of the CAPN530 Polymorphism in Slovak Simmental Bulls
... The high-resolution melting analysis is a cost-effective and simple post-PCR technique that can be used for SNP ...the high resolution melting analysis for ...
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Profiling of adulterant in tongkat ali herbal product using dna barcoding in combination with high resolution melting analysis
... of High Resolution Melting Analysis (Bar- ...obtain high quality genomic DNA, extraction was done using modified Cetyl Trimethyl Ammonium Bromide (CTAB) method and Nucleospin Plant II ...
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Evaluation of High Resolution Melting analysis as an alternate tool to screen for risk alleles associated with small kidneys in Indian newborns
... polymorphism analysis, denaturing gradient gel electrophoresis, temperature gra- dient capillary electrophoresis, restriction enzyme diges- tion of polymerase chain reaction (PCR) products and sequencing ...cost. ...
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A rational two-step approach to KRAS mutation testing in colorectal cancer using high resolution melting analysis and pyrosequencing
... mutation analysis in mCRC is- sued by the ...the high incidence of CRC resulting in high demand for KRAS mutation testing, an ideal diagnostic assay for this purpose not only needs to be sufficiently ...
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High resolution melting analysis reveals low Plasmodium parasitaemia infections among microscopically negative febrile patients in western Kenya
... Purity N Kipanga 1,3 , David Omondi 1,4 , Paul O Mireji 5 , Patrick Sawa 2 , Daniel K Masiga 1 and Jandouwe Villinger 1* Abstract Background: Microscopy and rapid diagnostic tests (RDTs) are common tools for diagnosing ...
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Evaluation of spa-typing of methicillin-resistant Staphylococcus aureus using high-resolution melting analysis
... of high-resolution melting analysis for rapid spa typing on methicillin- resistant Staphylococcus aureus clinical ...F. High-resolution melting analysis of the spa ...
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Rapid Detection of Isoniazid, Rifampin, and Ofloxacin Resistance in Mycobacterium tuberculosis Clinical Isolates Using High Resolution Melting Analysis
... A high-resolution melting analysis (HRMA) assay was developed to detect isoniazid, rifampin, and ofloxacin resistance in Mycobacterium tuberculosis by targeting resistance-associated mutations ...
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Rapid Detection of Common HIV 1 Drug Resistance Mutations by Use of High Resolution Melting Analysis and Unlabeled Probes
... efficacy. High- resolution melting analysis (HRMA) has been used to successfully identify single nucleo- tide polymorphisms (SNPs) and to genotype viral and bacterial ...the melting ...
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A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars
... for high resolution melting analysis due to the technique being highly dependent on salt and buffer conditions in a reaction, our routine analyses were usually performed on individual DNA ...
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High-Resolution Melting Analysis for Screening of Turkish Germline Mutations in BRCA1 and BRCA2
... HRM Analysis Upon completion of the run (approximately 2 ho- urs), HRM curve analysis was performed using the LightCycler ® 480 Software version ...The melting curves were normalized, to allow ...
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Development of a Rapid High Throughput Method for High Resolution Melting Analysis for Routine Detection and Genotyping of Noroviruses
... rapid, high-throughput detection and genotyping method for noroviruses using real-time reverse transcription-PCR (RT-PCR) and high-resolution melting (HRM) analysis to create a ...
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High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis
... Blood samples comprising both field-collected samples and samples from experimentally infected cattle were used to evaluate and compare HRM with the identification results using nested PCR and 18S rRNA gene sequencing. ...
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High resolution melting analysis for molecular detection of multidrug resistance tuberculosis in Peruvian isolates
... curve analysis as a method for the rapid and accurate detection of mutations associated to ...HRM analysis does not cover all mutations related to resistance, and thus, the technique cannot yet replace ...
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Rapid Detection of Rifampicin and Isoniazid Resistant Mycobacterium tuberculosis by High Resolution Melting Analysis
... a high-resolution melting (HRM) assay to scan for mutations in the rpoB, inhA, ahpC, and katG genes and/or promoter regions for the detection of rifampin and isoniazid resistance in Mycobacte- rium ...
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Identification of Phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation
... the high resolution melting (HRM) analysis of the Ypt1 gene non-coding regions was validated for ten Phytophthora species with a broad host range from forest trees to crop ...The ...
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High-Resolution Melting Analysis as an Appropriate Method to Differentiate between Fasciola hepatica and F. gigantica
... melting curve analysis was about 1˚C. This find- ing indicates a sufficient ability and reliability of the assay for distinguishing these two parasites. The real-time PCR and HRM assay often are able to ...
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Rapid Differentiation of Influenza A Virus Subtypes and Genetic Screening for Virus Variants by High Resolution Melting Analysis
... HRM analysis of the PCR products with the HR-1 instrument and used the melting profiles as molecular fingerprints for virus ...the high-resolution derivative plot obtained by heteroduplex ...
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Multiplex Real Time PCR Assay with High Resolution Melting Analysis for Characterization of Antimicrobial Resistance in Neisseria gonorrhoeae
... HRM analysis that accurately de- tected several important mutations associated with resistance to antibiotics commonly used to treat ...the high limit of detection suggested that our method is not suitable ...
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Authenticity testing and detection of Eurycoma longifolia in commercial herbal products using bar-high resolution melting analysis
... sensitive melting curve changes caused from the release of a saturated intercalating dye from DNA duplex denaturation by increasing the temperature ...HRM analysis is directly applied right after ...
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