High-resolution melting analysis (HRMA)
The Use of High Resolution Melting Analysis for Detection of the CAPN530 Polymorphism in Slovak Simmental Bulls
... The high-resolution melting analysis is a cost-effective and simple post-PCR technique that can be used for SNP ...the high resolution melting analysis for ...
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<p>Distribution of Class B and Class A β-Lactamases in Clinical Strains of <em>Pseudomonas aeruginosa</em>: Comparison of Phenotypic Methods and High-Resolution Melting Analysis (HRMA) Assay</p>
... High-Resolution Melting Analysis (HRMA) is an emerging technique used to discriminate DNA sequence ...8 HRMA is based on accurate determination of the relationship between ...
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Evaluation of High Resolution Melting analysis as an alternate tool to screen for risk alleles associated with small kidneys in Indian newborns
... polymorphism analysis, denaturing gradient gel electrophoresis, temperature gra- dient capillary electrophoresis, restriction enzyme diges- tion of polymerase chain reaction (PCR) products and sequencing ...cost. ...
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Amino acid permease 3 (aap3) coding sequence as a target for Leishmania identification and diagnosis of leishmaniases using high resolution melting analysis
... by high-resolution melting analysis (HRM), generates thermodynamic differences in the dissociation profile of amplicons resulting in specific signatures of polymorphisms due to small ...
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Identification of Ixodes ricinus blood meals using an automated protocol with high resolution melting analysis (HRMA) reveals the importance of domestic dogs as larval tick hosts in Italian alpine forests
... the HRMA protocol to a large number of tick nymphs from across the Province of Trento has lent insight into the feeding ecology of larval ticks, and TBDs risk in this ...
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Comparative Analysis of Two Broad Range PCR Assays for Pathogen Detection in Positive Blood Culture Bottles: PCR–High Resolution Melting Analysis versus PCR Mass Spectrometry
... PCR assays have relatively short turnaround times (1), can provide detailed pathogen characterization, and may detect or- ganisms missed by culture (1, 7, 25). By using primers that target universally conserved ...
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Identification of Phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation
... The high differ- entiation power of the Ypt1 gene upon Phytophthora species is a consequence of the high variation in the non-coding regions of this gene (Schena & Cooke ...
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High resolution melting analysis for molecular detection of multidrug resistance tuberculosis in Peruvian isolates
... HRM analysis To evaluate the ability of the HRM assay to detect rifampi- cin resistance, we analyzed sequence variations in a 118 bp region of the rpoB gene on the DNA samples of 167 Mtb ...(HRM analysis) ...
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High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis
... The high-resolution melting (HRM) assay is a novel and powerful molecular method applied to mutation detection, genotype analysis and species identifica- tion ...temperature, melting ...
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Rapid Detection of Rifampicin and Isoniazid Resistant Mycobacterium tuberculosis by High Resolution Melting Analysis
... All PCRs were performed in duplicate. The PCR cycling and HRM analysis were performed on the Rotor-Gene 6000 (Corbett Research, Sydney, Australia). PCR cycling parameters were as follows: 95°C for 15 min, 40 ...
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Development of a Rapid High Throughput Method for High Resolution Melting Analysis for Routine Detection and Genotyping of Noroviruses
... the high-resolution melting (HRM) assay, we selected stool samples that were determined by RT-PCR and sequenc- ing to be positive for six noroviruses belonging to the dominant subtypes that have been ...
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High resolution melting analysis for the detection of EMS induced mutations in wheat SBEIIa genes
... characterization of a barley waxy mutant, derived by mutagenesis, in which a premature stop codon was associated to the absence of the protein product; in this case the transcript level of the mutant allele was found ...
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Molecular detection of rifampin, isoniazid, and ofloxacin resistance in Iranian isolates of <em>Mycobacterium tuberculosis</em> by high-resolution melting analysis
... and high-resolution melting (HRM) curve analysis for the determination of resistance to rifampin (RIF), isoniazid (INH), and ofloxacin (OFX) in MTB isolates and to investigate their ...
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High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes
... average melting peaks detected by the Eco cycler system did not differ by more than ...age melting peaks detected with the Light cycler system. However, the melting peaks detected with the Light cy- ...
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The mutation profile of JAK2 and CALR in Chinese Han patients with Philadelphia chromosome-negative myeloproliferative neoplasms
... High-resolution melting analysis (HRMA) was used to screen for genetic alternations in JAK2 Exon ...a high mutation detecting sensitivity ...the HRMA assay on a Rotor-Gene ...
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Mutation analysis of genes in the EGFR pathway in Head and Neck cancer patients: implications for anti EGFR treatment response
... by high resolution melting analysis (HRMA), whereas EGFRvIII was determined using one-step real-time ...Survival analysis was performed using Kaplan-Meier analysis and ...
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High Resolution Melting Curve Analysis for Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis: a Meta Analysis
... on HRMA for the detection of RIF resistance shows that HRMA has high sensitivity and specificity when applied to ...of HRMA for RIF resistance detection is slightly higher than that of ...
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Genotyping of SNPs associated with meat tenderness: comparison of two PCR-based methods.
... that high-resolution melting (HRM) analysis is a simple and sensitive approach to genotyping SNPs, being comparable or superior to other methods (Liew, 2004; Reed and Wittwer, 2004; Reed et ...
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Rapid analysis of heterogeneously methylated DNA using digital methylation-sensitive high resolution melting: application to the CDKN2B ( p15 ) gene
... earlier melting of heteroduplexes relative to ...resulting melting curve is flattened and exhibits a complex melting ...a melting profile that cannot be directly compared with methylated and ...
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High Resolution Melting Curve Analysis for Rapid Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis Clinical Isolates
... (6/55). High rates of PZA resistance among MDR-TB have been observed in previous studies, ranging from 49% in Thailand [13], to 50% in Cen- tral Africa [14], 52% in South Africa [15], 53% in Japan [16] and 55% in ...
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