High-throughput Screening Assay for DHFR activity
Investigation of the antimicrobial activity of soy peptides by developing a high throughput drug screening assay
... [12] . Therefore, the combination of amino acids in the sequences of peptides could have affected their antibacterial ef ficacy. It has been demonstrated that some cations neutralize re- pulsive forces of adjacent ...
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A High-Throughput Assay for Screening of Natural Products that Enhanced Tumoricidal Activity of NK Cells
... Andrographolide, one among the 28 natural products had been demonstrated to have anti-inflammatory, anti- cancer and angiogenesis activities both in vitro and in vivo [22, 36]. In this study, andrographolide was shown to ...
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Neuraminidase activity provides a practical read out for a high throughput influenza antiviral screening assay
... neutralization assay to develop a practical, cell-based, high throughput screening ...This assay uses viral neuraminidase (NA) as a read-out to quantify influenza replication, thereby ...
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Potential High-Throughput Assay for Screening Inhibitors of West Nile Virus Replication
... the assay does not include targets involved in viral entry, genome encapsidation, and virion ...line assay is that inhibitors iden- tified through such a cell-based assay should have a high ...
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High-Throughput Microsomal Stability Assay for Screening New Chemical Entities in Drug Discovery
... Slightly more variability could be expected in the pooling methods as pooled analytes are diluted by a factor of 2 or 4, and accuracy could be affected by analyzer sensitivity. For a single time point measurement, the ...
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A cell-based luciferase assay amenable to high-throughput screening of inhibitors of arenavirus budding
... based assay to assess the budding of Ebola virus VP40 (McCarthy et ...luciferase activity associated with VLP was due to incorporation of FLuc into VP40 and GP- containing VLP by mechanisms that remain ...
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A piggyBac-based TANGO GFP assay for high throughput screening of GPCR ligands in live cells
... piggyBac-TANGO assay. The principle of the piggyBac-TANGO assay is es- sentially the same as the general concept developed by Barnea et ...to high signal-to-background ratio, stable readout and ...
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A Novel, Sensitive Assay for High Throughput Molecular Detection of Plasmodia for Active Screening of Malaria for Elimination
... the throughput and the sensitivity ...hybridization assay to detect genus Plasmodium 18S rRNA directly from whole-blood samples from Plasmodium falciparum and Plasmodium vivax patients without RNA ...the ...
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High throughput screening in niche based assay identifies compounds to target preleukemic stem cells
... for high drug attri- tion rates when it comes to efficacy in clinical trials ...this high attrition rate, our results suggest that cell line–based screen- ing as well as 2-dimensional screening ...
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Visual and computational analysis of structure-activity relationships in high-throughput screening data
... biological activity, with the results of such experiments being fed back to inform the selection of further ...in high-throughput screening (HTS) [6-8] mean that such operations have been ...
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Development of a Screening Assay for Type III Secretion System Inhibitors and High Throughput Screening Campaign of Inhibitors of PRP of Staphylococcus aureus
... 14 determines the hierarchal order of protein secretion. 123 It is believed that EscU participates in a similar fashion in substrate switching for non-flagellar type III secretion. 125 The inner membrane components of ...
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Robust High Throughput Real time Monitoring Assay for the Specific Screening of Bacterial Cell Envelope Inhibitors
... automated high-throughput real-time cell viability monitoring assay using Sytox green ...Our assay requires no manual intervention during bacterial cell ...the assay is very specific ...
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Microfluidic Technologies for High-Throughput Screening Applications
... of screening tens of thousands of compounds in a 24-hour ...develop assay systems that are smaller, faster, and cheaper has been realized in the field of ...
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A high-throughput chemically induced inflammation assay in zebrafish
... express high levels of matrix metalloproteinase 9 (mmp9), a marker of myeloid lineage cell populations ...their activity as they potently inhibit leuko- cyte infiltration of the ...
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A novel high throughput assay for anthelmintic drug screening and resistance diagnosis by real-time monitoring of parasite motility
... to screening 105 initial hits against adult stage parasites ...the screening alone took two full time researchers one month of training to identify phenotypes, three months to complete the primary screen ...
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Luciferase-Based, High-Throughput Assay for Screening and Profiling Transmission-Blocking Compounds against Plasmodium falciparum Gametocytes
... cost-effective high- throughput reporter gene assay to identify compounds active against late-stage (stage IV and V) ...The assay, which is suitable for testing compound activity at ...
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An integrated droplet based microfluidic platform for high throughput, multi-parameter screening of photosensitiser activity
... iv ABSTRACT With rapid advances in the field of cellomics, genomics, and proteomics, the demands for development of enabling technologies for performing high throughput biological experimentation are ever ...
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An exposure:activity profiling method for interpreting high-throughput screening data for estrogenic activity—Proof of concept
... endocrine screening, such as within EPA’s tiered testing and assessment approach, the Endocrine Disruptor Screening Program ...HTS assay data from the ToxCast™ and Tox21 programs; these efforts were ...
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Developing High-Throughput HIV Incidence Assay with Pyrosequencing Platform
... base—a rate less than or comparable to that in previous reports which relied on a reference sequence and neighborhood base calls to correct errors or which utilized the Bayesian posterior proba- bility of the homopolymer ...
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A high-throughput assay to identify modifiers of premature chromosome condensation.
... Operetta high-throughput screening (HTS) microscope and Harmony/Columbus software could achieve ...low throughput and logistically unsuitable for application to a high-throughput ...
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