HIV-1 entry inhibitor
Trimeric Glycosylphosphatidylinositol-Anchored HCDR3 of Broadly Neutralizing Antibody PG16 Is a Potent HIV-1 Entry Inhibitor
... type 1 (HIV-1) envelopes of subtypes A, B, B=, C, and A/E (15–19) and a 10A1 retroviral envelope (20), and a panel of HIV and simian immunodeficiency virus (SIV) were used to infect GPI-HCDR3- ...
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Punica granatum(Pomegranate) juice provides an HIV 1 entry inhibitor and candidate topical microbicide
... for 1 h at 37°C. The wells were washed 5 × with TS. Biotinyl-CD4 (1 µg/ml) in TS-1% gelatin was added to the wells for 5 h at ...washing 1 × with ...MO; 1 µg/ml in TS-10% goat ...on ...
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Design of a Potent d-Peptide HIV-1 Entry Inhibitor with a Strong Barrier to Resistance
... of HIV, we measured the potency of PIE7-trimer, PIE12-trimer, and PIE12 (with enfuvirtide as a control inhibitor) using the PhenoSense Entry pseudovirion assay (Monogram Bio- sciences) (Table 3) ...
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Progress in development of HIV 1 integrase inhibitor
... cell HIV infection is called a T helper ...system. HIV infects the T Helper cell because it has the protein CD4 on its surface, which HIV uses to attach itself to the cell before gaining ...cell, ...
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Enfuvirtide (T20)-Based Lipopeptide Is a Potent HIV-1 Cell Fusion Inhibitor: Implications for Viral Entry and Inhibition
... fusion inhibitor used in combination therapy of HIV-1 infections; however, T20 has several defects that limit its clinical use, including a low genetic barrier for drug resistance and a short in vivo ...
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HIV-1 Resistance to the Capsid-Targeting Inhibitor PF74 Results in Altered Dependence on Host Factors Required for Virus Nuclear Entry
... to HIV-1 ...measure HIV-1 infectivity and antiviral inhibition were performed by luciferase assay in TZM-bl re- porter cells, as previously described ...
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Cell type specific requirements for thiol/disulfide exchange during HIV 1 entry and infection
... on HIV-1 infection of primary human cells [16,19,26], our current study set out to provide a comprehensive inves- tigation of the role of thiol/disulfide exchange in HIV-1 infection using ...
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Human Erythrocyte Glycosphingolipids as Alternative Cofactors for Human Immunodeficiency Virus Type 1 (HIV-1) Entry: Evidence for CD4-Induced Interactions between HIV-1 gp120 and Reconstituted Membrane Microdomains of Glycosphingolipids (Gb3 and GM3)
... (Table 1). The specificity of 39- sialyllactose as an inhibitor of GM3-CD4 association is dem- onstrated by the incapacity of the oligosaccharide to affect the interaction of cholera toxin with its ...
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HIV 1 capsid is involved in post nuclear entry steps
... inhibit HIV-1 infection by targeting capsid have been described ...competitive inhibitor of CPSF6 and Nup153 interaction with capsid [18, 28] and appears to act post- nuclear entry ...the ...
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Suramin is a potent inhibitor of Chikungunya and Ebola virus cell entry
... vector entry [15–17]. As a competitive inhibitor of GAGs and heparin, suramin has been shown to have anti-viral ...them HIV [18, 19] HSV-1 [20], HBV [21], HCV [22], dengue virus [23], EV71 ...
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A novel small-molecule inhibitor of HIV-1 entry
... assays, we observed that 5660386 was able to potentiate the CCR5-antagonist MVC but not BMS (Figure 2), suggest- ing that 5660386 does not compete with MVC for binding to CCR5. In addition, 5660386 did not inhibit ...
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Novel Acylguanidine-Based Inhibitor of HIV-1
... Canadian HIV Cure Enterprise Team grant HIG-133050 from CIHR, in partnership with CANFAR and IAS (to ...(CANSSA) HIV/AIDS Network, through funding pro- vided by the Global Health Research Initiative, itself ...
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Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors
... The inhibitor concentrations used in these studies were higher than those previously published because C-terminally truncated Envs have a fast fusion kinetic and are thus less sensitive to entry inhibitors ...
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Characterization of resistance to a potent d peptide HIV entry inhibitor
... Additional file 1. Mutations observed in PIE12-trimer resistant viral pools. Env gene position (pNL4-3 numbering), reference base, and all base calls for control and PIE12-trimer resistance viral populations with ...
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Reversible and Efficient Activation of HIV-1 Cell Entry by a Tyrosine-Sulfated Peptide Dissects Endocytic Entry and Inhibitor Mechanisms
... infectious HIV-1 virions leave cell ...to 1) that depends on the struc- ture and cell surface concentration of the coreceptor ...face entry steps are completed ...final entry occurs ...
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Closing two doors of viral entry: Intramolecular combination of a coreceptor and fusion inhibitor of HIV 1
... against HIV-1 ...the entry process involved and thus may potentially be more potent then the constructs containing the ...fusion inhibitor (BFFI) was expressed transiently in human embryonic ...
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Insights into the Role of Chemokines and Chemokine Receptors During HIV-1 Pathogenesis
... In addition to using CCR5 and being T-cell tropic, several other phenotypic and genotypic traits have been associated with T/F Env proteins relative to those from chronic viruses (56, 73, 131, 200, 234, 235, 238, 239, ...
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M48U1 CD4 mimetic has a sustained inhibitory effect on cell associated HIV 1 by attenuating virion infectivity through gp120 shedding
... epithelium that lines the female genital tract. However, whether the initial microbicide exposure can affect virus budding from these migrating seminal leucocytes remains unclear. Therefore, we here evaluated the effect ...
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Virion stiffness regulates immature HIV 1 entry
... viral entry measurement The viral entry assay was performed as described ...viruses, 1 μM ß-lactamase sub- strate solution (CCF2-AM, Invitrogen) was incubated with cells at 13°C for 17 ...the ...
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Differential Ability of Primary HIV-1 Nef Isolates To Downregulate HIV-1 Entry Receptors
... 70% and reduced downregulation activities of CD4 and CCR5 also by 70%. The downregulation of HLA class I was similarly disrupted by this mutation (data not shown). Therefore, the re- duced Nef function is most likely ...
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