HIV-1 gp120
Local Conformational Stability of HIV-1 gp120 in Unliganded and CD4-Bound States as Defined by Amide Hydrogen/Deuterium Exchange
... the HIV-1 gp120 envelope glycoprotein to the CD4 receptor involves exceptional changes in enthalpy and ...of gp120 in unliganded and various ligand-bound states, meanwhile, reveal an inner ...
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The HIV-1 gp120 Major Variable Regions Modulate Cold Inactivation
... protein gp120 and transmembrane glycoprotein gp41, which are organized into trimeric spikes on the surface of the virus ...(1–3). HIV-1 entry is initiated by interaction of gp120 with ...
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Synthetic multimeric peptides derived from the principal neutralization domain (V3 loop) of human immunodeficiency virus type 1 (HIV-1) gp120 bind to galactosylceramide and block HIV-1 infection in a human CD4-negative mucosal epithelial cell line.
... type 1 (HIV-1), the etiologic agent of AIDS (15, ...non-CD4 HIV-1 receptor has been identified recently on CD4 2 brain-derived cells (2, 14) and in colonic epithelial cells (9, 31, ...
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Human CNS cultures exposed to HIV-1 gp120 reproduce dendritic injuries of HIV-1-associated dementia
... The HIV-1 envelope glycoprotein gp120 has been linked to the pathogenesis of HAD from several lines of ...and gp120 alone have been shown to be toxic to murine, avian and human CNS cultures ...
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Extracellular Matrix Proteins Mediate HIV-1 gp120 Interactions with α4β7
... 37. McLellan JS, Pancera M, Carrico C, Gorman J, Julien JP, Khayat R, Louder R, Pejchal R, Sastry M, Dai K, O’Dell S, Patel N, Shahzad-ul Hussan S, Yang Y, Zhang B, Zhou T, Zhu J, Boyington JC, Chuang GY, Diwanji D, ...
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Antioxidant protection from HIV-1 gp120-induced neuroglial toxicity
... to gp120 (Iskander et al.: Human CNS cultures exposed to HIV-1 gp120 reproduce dendritic injuries of HIV-1-associ- ated ...following gp120 exposure comes in part from ...
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Allosteric induction of the CD4 bound conformation of HIV 1 Gp120
... of gp120 CDC451 to each of the phage-displayed peptides were performed at a concentrations ranging between ...concentrations. gp120 CDC451 was injected over the immobilized phages for 2 min at a flow rate ...
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Human Erythrocyte Glycosphingolipids as Alternative Cofactors for Human Immunodeficiency Virus Type 1 (HIV-1) Entry: Evidence for CD4-Induced Interactions between HIV-1 gp120 and Reconstituted Membrane Microdomains of Glycosphingolipids (Gb3 and GM3)
... GM3 are colocalized in the same detergent-insoluble microdo- main (28). Using a reconstituted membrane patch of GM3 at the air-water interface, we recently demonstrated that soluble CD4 interacts with GM3 and that the ...
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Isolate-Specific Differences in the Conformational Dynamics and Antigenicity of HIV-1 gp120
... each gp120 sample were linear, confirming that the SAXS data from size ex- clusion chromatography-eluted samples were free of artifacts due to aggregation or interparticle effects and hence suitable for fur- ther ...
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Conformational alterations in the CD4 binding cavity of HIV 1 gp120 influencing gp120 CD4 interactions and fusogenicity of HIV 1 envelopes derived from brain and other tissues
... primary gp120 proteins and the subsequent influence on Env function, we recently developed and validated a protocol to produce and utilize three-dimensional structural models of gp120 to deduce CD4bs ...
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Staphylococcus aureus Leukocidin LukED and HIV 1 gp120 Target Different Sequence Determinants on CCR5
... a 1:100 dilution in TSB supplemented with chloramphenicol (10 g/ml) and in- cubated for 5 h at 37°C with shaking at 180 ...in 1 ⫻ Tris-buffered saline (TBS; Cellgro) for 30 min at 4°C while ...with ...
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Differences in the mannose oligomer specificities of the closely related lectins from Galanthus nivalis and Zea maysstrongly determine their eventual anti HIV activity
... for HIV-1 ...the HIV gp120 envelope protein as a prerequisite to exhibit pronounced antiviral ...on gp120 is based on the study of Leonard et ...expressed gp120, it is well ...
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Use of silver nanoparticles increased inhibition of cell-associated HIV-1 infection by neutralizing antibodies developed against HIV-1 envelope proteins
... HIV-1 infection was made with a luciferase-based assay. The percentage of residual infectivity after treatment was calculated with respect to the positive control of untreated virus. The assay was performed ...
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Release of gp120 Restraints Leads to an Entry Competent Intermediate State of the HIV 1 Envelope Glycoproteins
... primary HIV-1 isolates exhibit high rates of binding to Env trimers in state 1 (41, 57, ...state 1 and downstream conformations are lowered, increasing the propensity for the CD4-bound and ...
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HLA C increases HIV 1 infectivity and is associated with gp120
... insensitive gp120/gp41s identified, NDK (clade D) and J500 (clade B), with the sequences of the HLA-C sensitive Envs, revealed 3 identical aminoacid substitu- tions in the V3 loop, which were absent in all other ...
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Molecular mimicry between the human immunodeficiency virus type 1 gp120 V3 loop and human brain proteins.
... Western blot immunoblot analysis of the brain proteins was performed with several monoclonal and polyclonal anti-HIV-1 antibodies including polyclonal rabbit anti-HIV-1 gp120 American Bi[r] ...
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A Replication-Competent, Neutralization-Sensitive Variant of Simian Immunodeficiency Virus Lacking 100 Amino Acids of Envelope
... SIV gp120 pro- tein has not been reported, it is possible to map SIVgp120 residues onto the recently published structure of HIV-1 gp120 ...the gp120 sequence of SIVmac239⌬V1V2(d119) are ...
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Discovery of Small-Molecule Human Immunodeficiency Virus Type 1 Entry Inhibitors That Target the gp120-Binding Domain of CD4
... the gp120-CD4 in- teraction involves relatively small contact surface areas on both ...of HIV-1 gp120 core com- plexed with an N-terminal two-domain CD4 (D1/D2 CD4) and a Fab fragment of a ...
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Human Immunodeficiency Virus Type 1 Uses Lipid Raft-Colocalized CD4 and Chemokine Receptors for Productive Entry into CD4+ T Cells
... destabilizing HIV-1 parti- cles and preventing virus entry, seems less likely, since very low levels of cyclodextrin, if any, were shown to associate with cells ...10. HIV-1 binding to PM1 T ...
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HIV 1 envelope, integrins and co receptor use in mucosal transmission of HIV
... of gp120 to both CCR5 and CXCR4, activates several intracellular signaling cascades, mimicking the natural ligands of the chemokine recep- ...tors. HIV-1 gp120 has also been shown to trigger ...
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